CeO2- and Mn3O4-based nanozymes exhibit scavenging of singlet oxygen species and hydroxyl radicals

Abstract

Singlet oxygen and hydroxyl radicals are highly reactive species that contribute significantly to oxidative stress-related pathologies. Herein, we report the effective scavenging of ¹O₂ and ˙OH by CeO₂- and Mn₃O₄-based nanozymes and compare them with the well-known scavengers of these radicals. The IC₅₀ values of scavenging of ˙OH by these nanozymes were compared with NAC, which were in the order of Mn₃O₄ [2.3 μM] < CeO₂ [5.4 μM] < NAC [28.4 μM]. Similarly, the IC₅₀ values for ¹O₂ scavenging were in the order of Mn₃O₄ [4.75 μM] < sodium azide [60.5 μM] < CeO₂ [857.27 μM]. The cell viability assays, ROS generation studies and cell cycle analysis revealed that these nanozymes (1 μg mL⁻¹) are biocompatible with mammalian cells.