Issue 20, 2025

Anticancer activity: synthesis, characterization and DNA binding of a 1,2,4-triazole-based Zn(ii) complex

Abstract

A new zinc(II) complex, [Zn(L)2Cl2], with a 1,2,4-triazole-based ligand (L) was synthesized, and its origin was validated. The ligand and complex were formulated using priming to explore their DNA binding and anticancer activity. Moreover, they were characterised via single-crystal X-ray analysis. The complex crystallized in the triclinic space group P[1 with combining macron] and revealed a tetrahedral structure with 1 : 2 stoichiometry. Thermogravimetric analysis (TGA) conducted in a nitrogen environment at temperatures ranging from 25 °C to 500 °C revealed that the compound remained thermally stable up to almost 250 °C. DNA binding studies of the synthesised complex and its ligand with CT-DNA were conducted using fluorescence and UV-Vis spectroscopic techniques. The complex and ligand were bound to CT-DNA through intercalation, and their intrinsic binding constants (Kb) were 2.89 × 103 and 1.05 × 104 M−1 and Stern–Volmer binding constants (KSV) were 1.55 × 102 and 4.90 × 103 M−1, respectively. Additionally, the bio-efficacy of the ligand and its complex was examined in vitro against cancer cell lines—HeLa, MCF-7 and African green monkey kidney cells (Vero)—to assess their cytotoxicity.

Graphical abstract: Anticancer activity: synthesis, characterization and DNA binding of a 1,2,4-triazole-based Zn(ii) complex

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Article information

Article type
Paper
Submitted
24 Dec 2024
Accepted
08 Apr 2025
First published
28 Apr 2025

New J. Chem., 2025,49, 8215-8224

Anticancer activity: synthesis, characterization and DNA binding of a 1,2,4-triazole-based Zn(II) complex

Y. Gnanadeepam, B. Shankar and A. Tamilselvi, New J. Chem., 2025, 49, 8215 DOI: 10.1039/D4NJ05463F

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