From the journal:

Chemical Communications

Self-propagating amplification with a ss-polyadenine probe enables label-free detection of terminal deoxynucleotidyl transferase

Baoqiang Chen,a   Haidong Yang,a   Zhuqi Sui,a   Yuanyuan Yao, ORCID logo a   Longhua Guo ORCID logo *abc  and  Jianguo Xu ORCID logo *abc  

* Corresponding authors

a Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, China
E-mail: jgxu0816@163.com, guolh@zjxu.edu.cn

b G60 STI Valley Industry & Innovation Institute, Jiaxing University, Jiaxing, China

c Engineering Research Center of Intelligent Human Health Situation Awareness of Zhejiang Province, Jiaxing University, China

Abstract

We developed a label-free, self-propagating amplification strategy for ultrasensitive terminal deoxynucleotidyl transferase (TdT) detection. The system integrates TdT-triggered poly-T extension, poly-A-primed synthesis, nicking-mediated recycling, and SYBR Green I-based fluorescence output, achieving robust signal amplification and assay performance.

Graphical abstract: Self-propagating amplification with a ss-polyadenine probe enables label-free detection of terminal deoxynucleotidyl transferase

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Article information

DOI
https://doi.org/10.1039/D5CC04298D
Article type
Communication
Submitted
28 Jul 2025
Accepted
21 Aug 2025
First published
25 Aug 2025

Chem. Commun., 2025, Advance Article

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Self-propagating amplification with a ss-polyadenine probe enables label-free detection of terminal deoxynucleotidyl transferase

B. Chen, H. Yang, Z. Sui, Y. Yao, L. Guo and J. Xu, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC04298D

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