Construction of a novel highly selective NIR probe for monitoring the changes of glutathione levels in drug-induced liver injury

Abstract

Drug-induced liver injury (DILI) is a leading cause of acute liver failure, which is closely associated with oxidative stress. Glutathione (GSH), a vital sulfhydryl peptide, maintains cellular redox balance and signaling. In this study, we have successfully developed a highly selective near-infrared fluorescent probe, AH-F, which exhibits a 357-fold enhancement in fluorescence upon detection of GSH. With the aid of AH-F, the pertinent physiological parameters in a murine model were characterized by cellular and drug-induced liver injury, concurrently allowing for the assessment of the therapeutic efficacy of relevant pharmaceutical interventions.