Engineering a Mi3Chemout system for controlled recruitment and release of proteins of interest

Xiao Ma; Chunxue Liu; Lingqin Wang; Shijie Ren; Yongsheng Yu; Hong Yuan; Wei Kang; Chuang Xue

doi:10.1039/D5CC00808E

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Engineering a Mi3_Chemout system for controlled recruitment and release of proteins of interest†

Xiao Ma,

‡^abc Chunxue Liu,‡^b Lingqin Wang,^b Shijie Ren,^b Yongsheng Yu,^d Hong Yuan,*^a Wei Kang

*^abc and Chuang Xue

*^abc

Author affiliations

* Corresponding authors

^a Central Hospital of Dalian University of Technology, Dalian 116024, China
E-mail: yuanhong@dlut.edu.cn, kangwei@dlut.edu.cn, xue.1@dlut.edu.cn

^b MOE Key Laboratory of Bio-Intelligent Manufacturing School of Bioengineering, Dalian University of Technology, Dalian 116024, China

^c Ningbo Institute of Dalian University of Technology, Ningbo 315016, China

^d Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China

Abstract

Protein cages are ideal scaffolds for presenting proteins of interest (POIs), but controlled POI release remains challenging. We engineered the Mi3 protein cage with chemically inducible dimerization domains and a photocleavable protein, enabling controlled recruitment and release of POIs both in vitro and in E. coli cells.

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Article information

DOI: https://doi.org/10.1039/D5CC00808E
Article type: Communication
Submitted: 18 Feb 2025
Accepted: 06 Apr 2025
First published: 19 May 2025

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Chem. Commun., 2025, Advance Article

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Engineering a Mi3_Chemout system for controlled recruitment and release of proteins of interest

X. Ma, C. Liu, L. Wang, S. Ren, Y. Yu, H. Yuan, W. Kang and C. Xue, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC00808E

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Chemical Communications