Pyrococcus furiosus Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens

Jiahao Lin; Jiannan Zhang; Xianglin Zhu; Xuhan Xia; Yong Zhang; Qingdong Zeng; Yuanhong Xu; Ruijie Deng; Jinghong Li

doi:10.1039/D4CC06481J

Pyrococcus furiosus Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens†

Jiahao Lin,‡^abc Jiannan Zhang,‡^d Xianglin Zhu,^c Xuhan Xia,^c Yong Zhang,^c Qingdong Zeng,^f Yuanhong Xu,

^a Ruijie Deng

*^c and Jinghong Li

*^be

Author affiliations

* Corresponding authors

^a Institute of Biomedical Engineering, College of Life Sciences, Qingdao University, Qingdao 266071, China

^b Beijing Life Science Academy, Beijing 102206, China

^c College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China
E-mail: drj17@scu.edu.cn

^d Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China

^e Department of Chemistry, Center for BioAnalytical Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, New Cornerstone Science Laboratory, Tsinghua University, Beijing 100084, China

^f State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China

Abstract

Detection of low-abundance mutations for the early discovery of fungicide-resistant fungal pathogens is highly demanded, but remains challenging. Herein, we developed a dual-recognition strategy, termed PARPA, involving Pyrococcus furiosus Argonaute (pfAgo)-mediated elimination of wild-type fungal genes and CRISPR/Cas12a-based amplicon recognition. This assay can detect fungicide-resistant Puccinia striiformis at relative abundances as low as 0.05% and has potential for achieving early screening of fungicide-resistant fungal pathogens.

This article is part of the themed collection: CRISPR for Biosensing

Supplementary files

Article information

DOI: https://doi.org/10.1039/D4CC06481J
Article type: Communication
Submitted: 09 Dec 2024
Accepted: 21 Jan 2025
First published: 22 Jan 2025

Download Citation

Chem. Commun., 2025,61, 3335-3338

Permissions

Request permissions

Pyrococcus furiosus Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens

J. Lin, J. Zhang, X. Zhu, X. Xia, Y. Zhang, Q. Zeng, Y. Xu, R. Deng and J. Li, Chem. Commun., 2025, 61, 3335 DOI: 10.1039/D4CC06481J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Chemical Communications

Pyrococcus furiosus Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Pyrococcus furiosus Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens

Social activity

Search articles by author

Spotlight

Advertisements