Issue 25, 2025
From the journal:

Analytical Methods

A highly sensitive flow injection chemiluminescence method for myoglobin detection in beef samples based on zinc ion-induced “dragging” of heme iron†

Yali Zhang,a   Yuhan Ma, ORCID logo b   Yapin Zhaob  and  Jing Ha*b  

* Corresponding authors

a School of Sciences, Hebei University of Science and Technology, Shijiazhuang 050018, China
E-mail: zh_yali@hebust.edu.cn

b School of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
E-mail: hajing02@163.com

Abstract

We report for the first time that zinc ions (Zn2+) significantly enhance the chemiluminescence (CL) signal of the luminol–hydrogen peroxide (luminol–H2O2) system by “dragging” heme iron out of myoglobin (Mb). Integrating this with myoglobin aptamer (Mb aptamer) specific recognition enables sensitive and specific quantification of Mb content in beef samples.

Graphical abstract: A highly sensitive flow injection chemiluminescence method for myoglobin detection in beef samples based on zinc ion-induced “dragging” of heme iron

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Article information

DOI
https://doi.org/10.1039/D5AY00774G
Article type
Communication
Submitted
07 May 2025
Accepted
06 Jun 2025
First published
17 Jun 2025

Anal. Methods, 2025,17, 5155-5159

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A highly sensitive flow injection chemiluminescence method for myoglobin detection in beef samples based on zinc ion-induced “dragging” of heme iron

Y. Zhang, Y. Ma, Y. Zhao and J. Ha, Anal. Methods, 2025, 17, 5155 DOI: 10.1039/D5AY00774G

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