Endo IV-assisted cascade amplification for ultrasensitive TdT detection in acute lymphoblastic leukemia

Abstract

This study developed a highly sensitive fluorescence biosensor based on an Endo IV-fluorescent probe cascade amplification system, achieving a three-tier signal amplification. The detection limit is 9.6 × 10⁻⁵ U μL⁻¹, with a linear range of 0–0.3 U μL⁻¹. This technology offers excellent sensitivity, simplicity, and low cost, providing a powerful tool for the clinical diagnosis of acute lymphoblastic leukemia.