Issue 7, 2024

Insights into the time-course cellular effects triggered by iron oxide nanoparticles by combining proteomics with the traditional pharmacology strategy

Abstract

In recent years, a number of initially approved magnetic iron oxide nanoparticle (IONP)-based nano-medicines have been withdrawn due to the obscure nano-bio effects. Therefore, there is an urgent need to study the cellular effects triggered by IONPs on cells. In this study, we investigate the time-course cellular effects on the response of RAW 264.7 cells caused by Si-IONPs via pharmacological and mass spectrometry-based proteomics techniques. Our results revealed that Si-IONPs were internalized by clathrin-mediated endocytosis within 1 hour, and gradually degraded in endolysosomes over time, which might influence autophagy, oxidative stress, innate immune response, and inflammatory response after 12 hours. Our research provides a necessary assessment of Si-IONPs for further clinical treatment.

Graphical abstract: Insights into the time-course cellular effects triggered by iron oxide nanoparticles by combining proteomics with the traditional pharmacology strategy

Supplementary files

Article information

Article type
Paper
Submitted
20 Oct 2023
Accepted
15 Jan 2024
First published
02 Feb 2024

J. Mater. Chem. B, 2024,12, 1892-1904

Insights into the time-course cellular effects triggered by iron oxide nanoparticles by combining proteomics with the traditional pharmacology strategy

Y. Meng, Y. Liu, Y. Zhu, C. Qiu, A. Ma, D. Liu, S. Zhang, L. Gu, J. Zhang and J. Wang, J. Mater. Chem. B, 2024, 12, 1892 DOI: 10.1039/D3TB02476H

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