Teresa
Walter
a,
Manuela
Langbein
b,
Patrik
Blenk
b,
Alexander B.
Tesler
c,
Lucia H.
Prado
c,
Dan
Bornstein
c,
Sannakaisa
Virtanen
c,
Kathrin
Castiglione
*b and
Nicolas
Vogel
*a
aInstitute of Particle Technology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany. E-mail: nicolas.vogel@fau.de
bInstitute of Bioprocess Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91052 Erlangen, Germany. E-mail: kathrin.castiglione@fau.de
cChair of Surface Science and Corrosion, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany
First published on 26th March 2024
Biofouling, caused by the attachment of micro- and macroorganisms, increases the fuel consumption of ships and compromises aquatic infrastructure, especially by microbially induced corrosion. Conventional anti-fouling strategies often rely on the release of toxic substances. Liquid-infused coatings offer an alternative strategy, which has shown promising anti-fouling performance in maritime settings. In this study, we focus on freshwater systems and investigate the performance of a set of recently developed liquid-infused coatings with low environmental impact based on sustainable or non-toxic materials and energy-efficient fabrication. We combine field studies at two freshwater bodies in Bavaria, Germany with laboratory experiments focusing on corrosion and microbial attachment under controlled conditions. Our results indicate that liquid-infused coatings with low environmental impact can show a reduction in general biofouling, mussel attachment, and corrosion rates, as well as the attachment of Thiobacillus thioparus and Pseudomonas fluorescens, organisms known to cause microbially induced corrosion.
Mussels are especially concerning because of their particularly strong adhesion to many substrate materials, mediated by a durable natural glue based on dopa-enriched proteins.20 No synthetic glue is similarly unaffected by water or turbulent forces.21 The adhesion mechanism is an interplay of various mussel foot proteins (Mfps), and the strength depends on the curing of the polymer chains in the individual adhesion proteins.20,22 Thus, the removal of attached organisms is difficult and cost-intensive and the transport to different ecosystems is widespread.
The combination of fouling occurring both at the micro (i.e., bacteria) and macro-scale (i.e., mollusks) complicates the development of effective, broad anti-fouling approaches, as several length scales need to be simultaneously addressed. In the process, both fouling effects can be synergistically reinforced when microorganisms settle on the surface and provide adhesion points for bivalves by shielding the underlying surface chemistry.23 Thus, effective coating strategies must be able to prevent the adhesion of all fouling organisms.
Initial anti-fouling strategies were based on releasing toxins like tributyltin (TBT).2 However, as these biocides affect the environment, their application is prohibited in large parts of the world and should be generally avoided. TBT was found to cause severe damage to several aquatic species and thus was banned by the International Maritime Organization in 2008.24,25 Other chemically active coatings contain, for example, copper or zinc, which can also accumulate in invertebrates such as copepods and amphipods.26 To avoid such negative environmental impacts, coatings based on physical release mechanisms that prevent the adhesion of biological species without releasing potentially toxic species into the environment have been the focus of research activities.3,8,27 In the last decade, a new class of surface coatings, i.e., slippery liquid-infused porous surfaces (SLIPSs), emerged with promising anti-fouling capacities at all length scales,28–32 and significantly reduced adhesion strengths.32–36 SLIPS coatings operate by confining a thin film of a fluid lubricant within porous surface structures. If the involved interfacial energies are properly controlled,28,37–39 this liquid film is not replaced by a contaminating fluid or solid and thus shields the surface against the adhesion of liquids, contaminants, or organisms.31,36,37,40–42 Different variations of this strategy replace the underlying porous substrate with organogels,30,33,35,43 or grafted, liquid-like molecules.44–46
To this point, field studies underlined the performance of SLIPS coatings against biofouling, but have predominantly explored marine environments,32,34–36 while freshwater scenarios remain underexplored.33 While the shipping industry may be primarily affected by marine biofouling, freshwater systems host important water infrastructure, including floodgates, weirs, hydropower plants, and bridges. Freshwater infrastructure may be less prone to corrosion compared to chloride ion-rich sea water, but microbially induced corrosion and macrofouling can compromise the operation of such infrastructure and thus cause safety concerns. Therefore, an evaluation of the performance of liquid-infused coatings against biofouling in freshwater environments is important. A critical aspect to be considered when developing anti-fouling coatings to be used in freshwater infrastructure is the environmental impact, as such water bodies are often directly connected to drinking water supplies. These concerns have led to a more restrictive regulation of coatings to prevent the release of fluorinated or potentially harmful substances.
In this article, we explore the performance of a set of recently developed, environmentally friendly liquid-infused coatings based on sustainable or non-toxic materials against biofouling in German freshwater reservoirs. The coating processes are scalable, have a low environmental impact, and are fabricated with low energy consumption.47–50 We combine two field studies at different locations. Using a well-accessible lake at Tiergarten Nuremberg, Germany enables us to conduct a time-resolved study on general biofouling. At lake Roth (Rothsee), Bavaria, Germany, we explore the long-term performance of the coatings that were continuously submerged within the water body. This lake has been facing problems with the invasive species Dreissena polymorpha (zebra mussel) and Dreissena rostiformis bugensis (quagga mussel) for years and thus provides the perfect ecosystem to study the combined effects of fouling at all length scales. Additionally, we perform laboratory studies on electrochemical corrosion and the fouling of microorganisms known to induce microbial corrosion to give a more detailed insight into the protective properties of the tested coatings for biofouling-related applications.
Next, superhydrophobic coatings were applied on polycarbonate or black carbon steel (S355J2+N according to DIN EN 10025-2), cleaned by ultrasonication in ethanol (99.5% denaturated, Carl Roth) and water. The coatings were applied with a spray gun (Evolution Silverline Two in One, Harder & Steenbeck). Each coating was sprayed three times with a 30 s drying time in between each cycle to coat the substrates sufficiently and achieve superhydrophobicity. The spray nozzle was placed 15 cm from the substrate. After the coating process was completed, the samples were dried horizontally at room temperature. In the case of the coatings containing tung oil, an additional UV treatment (Bio-Link 365, Vilber, λ = 365 nm) of 6 h was applied to fully cure the binding material. Also, a primer layer was applied underneath the coating by spraying pure tung oil and cured for 1 h before applying the coating system, as this has been shown to increase the adhesion to the substrate and avoid delamination.48 Subsequently, the coated slides were washed by immersion in deionized water.
To form the slippery liquid-infused porous surfaces the superhydrophobic coatings were infiltrated using silicone (ipBMA, iTuSi) with a viscosity of 10 cSt or sunflower oil (iTuSu) with a viscosity between 60 and 70 cSt by spraying the respective oil on top of the coating three times.
All coatings were visually examined to ensure that they were uniform and characterized concerning their wetting properties via the contact angle before and after infiltration with the lubricant (to ensure superhydrophobic properties), and by contact angle hysteresis and sliding angles after infiltration with the lubricant. To provide quality control for all coatings used in this study, we used the established and published coating procedures in our lab (shown in ESI Fig. SI1†),47–50 determined reference values for all wetting parameters (contact angle, contact angle hysteresis, and sliding angles), which are listed in Table SI1,† and only used coatings that had parameters with the following deviations from these reference data: contact angle of non-infused surfaces: less than 7° deviation from the reference value; contact angle hysteresis: less than 3° deviation from the reference value; sliding angles: less than 3° deviation from the reference value.
For the two-step approach, the samples were first coated with a polydopamine (pDa) coating. This step was performed as follows. An aqueous solution containing 1 g L−1 dopamine HCl (≥98%, Sigma-Aldrich) and 10 mM tris(hydroxymethyl)aminomethane (TRIS, ≥99.9%, Carl Roth) was prepared and the samples were immersed with stirring at 900 rpm for 24 h. Afterward, the samples were rinsed with water and dried at room temperature. In a second step, the pDa-layer was functionalized for at least 48 h by adding silicone oil (15 μL cm−2) containing 20 mM monoaminopropyl-terminated polydimethylsiloxane (MAP-PDMS, MCR-A11, >95%, Gelest Inc.) and 20 mM triethylamine (TEA, 99%, Sigma Aldrich). In the course of this time, the MAP-PDMS reacts with the pDa coating to functionalize the surface in situ and thus hold the inert silicone oil layer in place.
The one-step approach was based on spray-coating a reactant mixture on the samples, which was then allowed to react for 96 h at room temperature. The mixture was prepared by first dissolving 0.04 g dopamine HCl in 40 mL tetrahydrofuran (THF, 99.9%, Carl Roth) and methanol (MeOH, ≥99.5%, Carl Roth) at a ratio of 1:
1 by volume. Next, 20 mM TEA, 20 mM MAP-PDMS, and 100 mM silicone oil were added. The reaction mixture was applied in seven spray cycles using a manual spray bottle at a 15 cm distance, 45° inclination, and with a 30 s pause in between the cycles.
Dividing bacteria were quantified by determination of colony forming units (CFU) on TSB agar plates in the case of P. fluorescens or on 290 S6 agar plates in the case of T. thioparus. For this purpose, plates containing 15 g L−1 agar were inoculated with serial dilutions of bacterial biofilm and incubated at 30 °C for two 2 or 10 days, respectively. After this time, colonies were counted, and CFU mL−1 was calculated. The statistical significance of observed differences in biofilm formation between the reference and coated samples was determined using an unpaired t-test for equal variances.
The first set of coatings includes three types of environmentally benign SLIPS coatings, formed from aqueous dispersions of a polymeric binder and hydrophobic silica particles. Together, these components form the porous structure into which the fluid lubricant is infused (Fig. 1a). The three samples differ in their components. Infused poly(butyl methacrylate) (ipBMA) coatings use silicone oil as the lubricant and constitute all-synthetic coatings.50 The polymeric binder can be replaced by naturally occurring tung oil (Tu), which is infused with silicone oil (Si) to form the liquid-infused iTuSi coatings.48 In this case, a first primer layer of pure tung oil was added to improve the incorporation of the silica particles and the substrate adhesion.48 Replacing synthetic silicone oil as the lubricant with natural sunflower oil (Su) forms the fully renewable iTuSu coating.48 Detailed schematics of the fabrication of these coatings are shown in Fig. SI1a,† and the development, optimization, and characterization of all coatings are reported in previous publications.48,50
The second set of coatings represents the class of infused, grafted liquid-like molecules, and consists of immobilized polydimethylsiloxane (PDMS) chains that are bound to the underlying surface to match the chemical nature of silicone oil used as the lubricant. Infused, functionalized polydopamine coatings (fpDa) take advantage of a pDa base layer, which is functionalized with monoaminopropyl-terminated polydimethylsiloxane chains to minimize the interfacial tension with silicone oil.47 Infused, UV-grafted PDMS (iUV-PDMS) is fabricated by irradiating a thin fluid silicone oil film to covalently anchor PDMS chains to the underlying surface.49 The detailed fabrication processes are shown in Fig. SI1b and c,† and coating process details and characterizations are reported in our previous publications.47,49
As a representative of the last class of liquid-infused coatings, we included silicone oil-infused PDMS (iPDMS) as an organogel widely used as a liquid-infused repellent coating,30,35,53 with a demonstrated performance against marine biofouling.32,35
For this study, we chose polycarbonate (PC) and black carbon steel (S355J2+N according to DIN EN 10025-2) as two relevant, but chemically different substrate materials. PC is often used e.g. in boat hulls, while carbon steel is the material used in pipes, and Bavarian aquatic infrastructure, such as pipes, rakes, floodgates, and weirs.
Fig. 2a exemplarily shows photographs of two representative samples throughout the field study. The uncoated PC reference (top row) experienced significant fouling already in week 4, which densified in the course of the study and completely covered the entire substrate. The infused poly(butyl methacrylate)-based SLIPS coating (ipBMA; bottom row), however, showed a much lower fouling coverage compared to the uncoated reference.
We quantitatively compared the biofouling of all tested samples by image analysis of the photographs taken every two weeks to determine the degree of coverage. Fig. 2b shows the evolution of biofouling determined from this image analysis. The ipBMA coatings outperformed the other environmentally friendly coatings and decreased the fouling by almost 80% compared to the uncoated reference. Similar trends were also found for coatings on glass substrates in a preliminary study in the fall of 2021 (Fig. SI3a and c†), underlining the broad adhesion of the ipBMA coating on different surfaces. A good performance was also observed for the iUV-PDMS coatings which reduced the fouling by 50% after week 12 (Fig. SI3b and d†), albeit only on glass substrates since it was not possible to coat PC substrates due to insufficient hydroxy groups on the polymeric substrate.
The iPDMS and iTuSi coatings showed a reduction in biofouling compared to the reference until week six, while afterward, the fouling increased drastically (Fig. 2b). In the case of the iPDMS, which has been shown to be very effective against marine biofouling,32 the loss of performance could be tracked to a detachment of the PDMS film from the underlying substrate. In a reference experiment included in this study, we used a glass substrate, where the adhesion to the PDMS layer is known to be better,32 and found iPDMS being efficient against biofouling (Fig. SI3a and c†), in agreement with reports in the literature on marine biofouling.32 Both the sunflower-oil-infused iTuSu coating and the functionalized polydopamine-based coating fpDa failed already after two weeks and showed comparable biofouling as the uncoated reference.
When comparing the three SLIPS-based coatings, we note that all coatings initially showed a large reduction in biofouling but eventually failed in their performance over time. ipBMA showed the best performance and only exhibited an onset of biofouling at the last time points of the study, while iTuSi showed a decreased performance from week 8, and iTuSu already failed after 4 weeks. While the exact origin of the failure cannot be determined from this study, we note that during this time-resolved study, the substrates were removed from the water at regular intervals to image and quantify the fouling process. This removal from the water phase induces a drying step and the creation of a three-phase contact line between the lubricated surface, water, and air associated with the meniscus of the water film receding from the surface. It has been shown in the literature that such three-phase contact lines deform the lubricant film, which forms wetting ridges along the water surface.54–56 These wetting ridges have been shown to be a major source of lubricant depletion, which is sheared off along with the meniscus of the receding water phase.57,58 Notably, this loss mechanism is enforced by the design of this field study by the periodic removal of the substrates. In these challenging conditions, the ability to retain the lubricant in these scenarios was highest for ipBMA, followed by iTuSi and iTuSu, which correlates with the decreasing hydrophobicity of the components of these coatings.48 This correlation suggests that the interfacial energies, which are tailored by matching the hydrophobicity of the surface texture and lubricant, can influence this physical removal of the lubricant. Following this hypothesis, an improved performance can be expected for an experimental design with a continuous submersion, as we will discuss below. Similar to the case of ipBMA, the iTuSi coating showed the same performance when coated on glass, again underlining the versatility of the coating process with respect to the underlying substrate (Fig. SI3e†). Lastly, despite efficient repellency properties under laboratory conditions,47 the fpDa coating showed no fouling decrease compared to the uncoated reference. We note however, that a chemically similar coating, prepared in a one-step process involving organic solvents,46 showed a decrease in biofouling of approximately 80% compared to an uncoated reference (Fig. SI3a and c†). In this case, the transfer to an environmentally benign approach was not successful against biofouling.
We first qualitatively evaluated the general biofouling from photographs taken at the end of the four-month immersion. Note that due to the dark color of the steel panels, a quantitative image analysis as in the time-resolved study at the Tiergarten pond (Fig. 2) was not possible. Fig. 3 a shows that the uncoated, bare steel reference exhibited pronounced biofouling. Fig. 3b shows exemplary photographs of the different environmentally friendly liquid-infused coatings. A decrease in fouling compared to the uncoated reference was seen for ipBMA and iTuSi but not for iUV-PDMS and fpDa, again in qualitative agreement with the study on PC substrates at the Tiergarten pond (Fig. 2).
Additionally, we found areas with evenly distributed mussels or the formation of mussel colonies on all fpDa, iUV-PDMS, and uncoated reference samples (Fig. 3a and b). We quantified this macrofouling based on mussel attachment by manually counting Dreissena mussels on all substrates (Fig. 3c). This quantification supports the qualitative impression of the samples. Firstly, the number of mussels attached to the fpDa substrate was in the same range as the number on the uncoated carbon steel substrate. iUV-PDMS showed even slightly higher numbers, indicating that these coatings did not prevent macrofouling. The number of mussels counted on the ipBMA substrates was approximately half of the count on the uncoated reference, showing an anti-fouling performance of this coating. The lowest numbers were found on the iTuSi coatings, with a reduction of ∼85% with respect to the uncoated reference. As hypothesized above, the improved performance of the iTuSi sample may be attributed to the continuous immersion in this study. In addition, this sample was also prepared using a tung oil primer layer to improve the integration and adhesion of the silica particles forming the porous support.48 Notably, despite a comparable overall coating thickness (∼2–3 μm)48,50 this primer layer may further protect the coating against fouling, compared to the ipBMA coating, either by partial swelling with silicone oil or by providing an enhanced physical barrier.
Summarizing both field studies, the environmentally friendly SLIPS coatings exhibited significant reductions of biofouling, which depended on environmental boundary conditions such as the removal from the water phase. Best performances were found for continuous immersion, where the developed SLIPS coatings provided efficient resistance to fouling even over extended periods of time. These coatings showed similar performances on all tested substrate materials. Liquid-infused coatings based on surface functionalization with silicone oil chains did not provide an efficient reduction of biofouling, except UV-PDMS coated on glass substrates. Our results align well with previous studies on lubricant-infused surfaces in different scenarios. These studies demonstrated that loss of lubricant mediated by liquid menisci is difficult to avoid and can lead to degradation of performance.55,57 When submerged in water, however, these studies showed the ability of liquid-infused coatings to retain repellent and self-cleaning properties over extended periods of time or large water volumes, for example in preventing protein adsorption59 or marine biofouling.32
In addition to corrosion observations from field studies, we investigated the corrosion protection ability of the different coatings via standard potentiodynamic polarization measurements in a 0.1 N sodium chloride solution (Fig. 4a). Since such electrochemical characterization requires sufficient conductivity of the electrolyte, the use of similar freshwater as in the field studies is not possible in our electrochemical experiments. We therefore chose controlled conditions with a fixed concentration of 0.1 M NaCl, an electrolyte that is present in natural waters, albeit at lower concentrations in the case of freshwater. The use of this more aggressive electrolyte also allows us to probe corrosion in shorter times compared to the field study, and therefore facilitates the detection of differences and the comparison between the involved samples with a diverse coating structure.
The polarization curve of the bare steel sample shows active dissolution, observable by a continuous increase of the current density in the anodic branch. At high anodic current densities, a diffusion-controlled regime is reached. For all coated samples, significantly lower anodic current densities can be observed, especially under moderate anodic polarization. This indicates that all coatings provide substantial corrosion protection, with notable differences in the first anodic current density plateau (indicating different barrier properties of the different coatings). The polarization curves moreover indicate that corrosion protection of the studied coatings is mostly provided by blocking the anodic metal dissolution reaction (as only minor decreases in the cathodic current densities are observed for the coated samples). It is noteworthy that for all coated samples, a steep increase of the anodic current densities occurs at approximately 0.375 V indicating a loss of the protective properties of the coatings – in agreement with previous observations for similar coatings.60 A second current increase is caused by oxygen evolution starting at 1.23 V (onset potential for water oxidation). To directly compare the corrosion protection efficiency of the different coatings under free corrosion conditions, the inhibition efficiency was calculated from the icorr values obtained from the polarization curves (as explained in the Experimental section). All coatings suppressed corrosion to some extent with the highest indicated corrosion inhibition efficiency (IE) of 95.6% for the iTuSi coating which showed a decrease in its corrosion current density Icorr by more than one order of magnitude. The lowest IEs of 75.3% and 76.8% were found with iTuSu and iUV-PDMS coatings, respectively. The ipBMA and fpDa coatings both showed an intermediate IE with 86.2 and 80.9%, respectively. The iUV-PDMS coating reached similar current densities to those of the uncoated reference at a potential of approximately 0.48 V, indicating that the coating detached or disintegrated at these voltages. At approximately 1.75 V the fpDa coating also reached the current density of the bare steel sample, indicating failure or decomposition of the coating. In both cases, defects within the coatings may also serve as attacking sites for chloride ions and the onset of pitting corrosion. As iUV-PDMS successfully prevented corrosion in previous studies49,60,61 we suspect that the lower amount of surface hydroxy groups on carbon steel may prevent a thorough anchoring of the PDMS chains in the process, compromising the quality of this type of coating on steel. Noteworthily, all other coated samples showed a reduced current density compared to the uncoated reference throughout the entire potential range probed in the experiment. Photographs of all samples after the potentiodynamic polarization support this interpretation (Fig. 4 d): iUV-PDMS and fpDa samples exposed to potentials of 2 V showed clear signs of corrosion which were comparable to those of bare black steel, even though corrosion occurs at a defective site only, whereas a fpDa sample exposed to only 1 V did not show any visible corrosion and retained its repellency properties. Samples coated with ipBMA, iTuSu, and iTuSi did not show macroscopic corrosion upon exposure to potentials up to 2 V and retained their repellency (Fig. 4d). When investigating the polarization curves in detail, spikes with higher current densities which rapidly decrease to lower current densities can be seen. We hypothesize that these spikes originate from localized attacks of chloride ions initiating pitting corrosion.62 Due to the self-healing effect of the liquid-infused coatings such localized corrosion initiation events were suppressed rapidly, recovering the lower current density. Microscopic examinations of the liquid-infused coatings after polarization gave direct evidence of such pitting corrosion (Fig. 4c).
Summarizing these experiments, all our developed, environmentally friendly liquid-infused coatings can suppress corrosion of carbon steel under mild conditions. The most effective corrosion resistance was achieved by the tung oil-based iTuSi coating. This increased performance, also compared to that of the synthetic, poly(butyl methacrylate) coating, may be caused by the properties of the polymer itself or the presence of a primer layer of bare tung oil that was incorporated in the coating.
The electrochemical corrosion studies do not provide information on the long-term protection ability of the coatings, and the possible influence of microbes present in natural waters on the corrosion performance is ignored in the simplified electrochemical investigations under laboratory conditions. Therefore, corrosion rates determined by long-term immersion experiments in field studies are not expected to be identical to corrosion rates determined by electrochemical measurements on freshly coated samples. However, electrochemical measurements are helpful for fast screening of the performance of the different coatings in comparison, and in addition reveal details on the origin of the observed corrosion protection effects (e.g., blocking of metal oxidation/dissolution vs. deceleration of reduction reactions).
For quantitative analysis of biofilms, there are several methods with individual advantages and challenges.69 In this study, a CFU mL−1 assay was used to quantify the number of cells of P. fluorescens and T. thioparus attached to steel coupons after growth periods of 4 and 12 days, respectively. This assay, also referred to as divisible cell counting, is based on the separation of individual cells on agar plates, which then grow into colonies that are visible without the aid of additional dyes or equipment. For this purpose, the biofilms were detached from the coupons by rinsing them with a specific volume of liquid. The cell suspensions were then plated out on agar plates at various dilution levels and analyzed.
As can be seen in Fig. 5a, all coated samples showed at least an 80% reduction in biofilm formation of T. thioparus compared to the uncoated reference samples. All reductions were statistically significant (p < 0.01). While the results of counted CFU were generally in agreement with the macroscopic evaluation of biofilm formation (Fig. SI4†), there was one exception: visual inspection revealed that the biofilm on the iUV-PDMS-coated coupons was clearly the most pronounced after the reference. Even after the biofilm was removed by rinsing, a slightly milky top layer was visible, which can be attributed to the strong attachment of the cells. Thus, incomplete biofilm removal may result in comparatively low values of CFU determination. Therefore, iUV-PDMS is the least suitable coating to prevent biofilms of this organism, which is in line with the poorer performance of this coating in the field studies and the electrochemistry experiments. For the uncoated reference, complete removal of the biofilm was also difficult (Fig. SI4†). However, this incomplete removal only leads to an underestimation of the adhering microbial counts, so the positive effect of the coatings tends to be underestimated and the reduction of biofouling is even more pronounced.
The effect of the different coatings on the biofilm formation of P. fluorescens was also investigated. In contrast to those of T. thioparus, the biofilms formed by these bacteria were easily removed from all coupons (Fig. SI5†). As summarized in Fig. 5b, the number of surface-attached cells was reduced by the different coatings around 50%. Apart from the iTuSu coating (p = 0.056), all reductions were statistically significant (p < 0.05). We suspect that the comparatively weaker effect of the coatings against P. fluorescens could be due to the well-known production of surface-active molecules by this organism.70 The secreted biosurfactants could change the surface chemistry of the coatings and thus compromise their repellency properties, leading to less pronounced biofilm prevention.
Although the extent of biofilm prevention differed between the two organisms, it is encouraging that the developed liquid-infused coatings outperformed the reference in both cases, suggesting that certain physicochemical properties of surfaces may impede the attachment of different bacterial groups. This is important since biofilms are usually composed of various microorganisms, which can also assist each other in adhering to a particular substrate. By producing adhesive molecules and inducing intercellular interactions, the coexistence of multiple species within a biofilm is promoted.71 Successful coating strategies should therefore ideally prevent or at least delay the attachment of various types of organisms.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4ta00741g |
This journal is © The Royal Society of Chemistry 2024 |