Maria Laura
Tummino
a,
Giuliana
Magnacca
b,
Monica
Rigoletto
b,
Mery
Malandrino
b,
Claudia
Vineis
a and
Enzo
Laurenti
*b
aInstitute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing, National Research Council of Italy (CNR-STIIMA), Corso G. Pella 16, 13900 Biella, Italy
bDepartment of Chemistry, University of Torino, Via P. Giuria 7, 10125 Torino, Italy. E-mail: enzo.laurenti@unito.it
First published on 29th May 2024
Hydroxyapatite, a mineral from the apatite group, is widely distributed in living organisms and largely studied because of its many properties, including the adsorption of many different substances. In this work, two functionalized nanohydroxyapatites were synthesized starting from their precursors (calcium hydroxide and phosphoric acid) in the presence of Fe(II)/(III) ions and bio-based substances (BBS) isolated from green compost. The products were characterized with different techniques (nitrogen adsorption/desorption, ATR-FTIR, XRD, TGA and ζ-potential measurements) and compared to nanohydroxyapatite obtained without further functionalization. The ability of these materials to remove different water pollutants by adsorption was tested using two organic dyes (crystal violet and methyl orange) and four inorganic ions, Al(III), Cr(III), Ni(II) and As(V), characterized by different ionic charges, dimensions and nature. Moreover, for the same purpose, the antibacterial properties of iron- and iron/BBS-added materials were also tested. The result showed the effective adsorption capability of the materials, in particular with respect to crystal violet, Al(III) and Cr(III), and an enhancement of adsorption capacity with respect to all the adsorbates after functionalization. Finally, the tests towards Staphylococcus aureus and Escherichia coli showed high antimicrobial activity for the bare nanohydroxyapatite samples, whereas the doping with iron and BBS or the high-temperature treatment remarkably impacted this capacity depending on the bacterial strain to eliminate.
Hydroxyapatites and nanohydroxyapatites have been proven to be excellent adsorbents for the removal and sequestration of heavy metals, actinides, radionuclides, halogen ions, and organics from wastewater thanks to their high stability under both oxidizing and reducing conditions and easy accessibility thanks to methods for industrial-scale production, which have significantly reduced the cost.10–12 In addition to the use in aqueous solutions, the addition of hydroxyapatite nanoparticles (nAp) as a soil improver reduced the mobilization of heavy metals and increased their geochemical stability in metal-contaminated soils.13 Possible sorption mechanisms include adsorption, ion exchange, surface complexation and precipitation (or dissolution–precipitation) of metal phosphates.12,14
The morphological and chemical properties of nanohydroxyapatites can be modified if the synthesis occurs in the presence of inorganic and organic additives.15 For example, the incorporation of iron into the hydroxyapatite structure is of great interest for the possibility of forming nanoparticles with magnetic properties and developing efficient adsorbent materials and catalysts that can be removed by applying a magnetic field.16,17 The use of organic substances as templates or, in general, modifiers in the synthesis of nanohydroxyapatites can significantly influence the morphology and surface chemistry of the final materials, causing a significant improvement in sorbent capacity.18,19 These strategies have been exploited by Mercado et al.,20,21 who synthesized paramagnetic iron-containing hydroxyapatite nanoparticles and paramagnetic materials further mixed with soluble bioorganic substances (SBOs, in particular, the so-called CVT230) derived from 230 day-composted urban waste as synthesis precursors. SBOs are mixtures of substances with different molecular weights, formed by long aliphatic C chains substituted by aromatic rings and COOH, NCO, CO, PhOH, O-alkyl, O-aryl, OCO, OMe, and alkyl amine functional groups.22,23 In the hydroxyapatite system, SBO introduction was found to increase Cu(II) and Pb(II) sorbent capacity.20,21
In this research, we deeply investigated the performance of similar materials through the adsorption of anionic and cationic dyes and other inorganic species, characterized by different ionic charges, radii and nature (metallic and semi-metallic), namely Al(III), Cr(III), Ni(II) and As(V). Indeed, it is essential to widen the application field of adsorbents and evaluate their decontamination capability in differently polluted wastewater.24–26 For the same purpose, we also investigated the antibacterial properties of iron- and iron/SBO-added materials, considering previous studies reporting such antimicrobial ability for different hydroxyapatites.27–33 The hydroxyapatite-based materials produced here are inspired by the studies of Mercado et al.,20,21 but the SBOs used as templates are compounds labeled bio-based substances (BBS), obtained by a slightly different procedure to CVT230 (lab-scale).22,34 The materials were characterized by a multi-analytical approach and the features after UV treatment and calcination were also assessed.
All the other chemical reagents were used as provided by the suppliers without further purification. In the experiments, if not otherwise specified, distilled water was used.
A similar process was used to synthesize nanohydroxyapatite with iron (nAp–Fe). A mixture of FeCl2·4H2O (1.27 g, >99 wt%, Sigma Aldrich), FeCl3·6H2O (1.79 g, 97 wt%, Sigma Aldrich) and phosphoric acid (4.44 g in 60 mL of H2O) was left under stirring for approximately 4 hours at a temperature of 60 °C to favor the complete dissolution of the iron salts. At the end of the dissolution process, the prepared solution was added dropwise to a suspension of calcium hydroxide (5 g in 40 mL of H2O) over a 2 h period at 80 °C under stirring. The reaction products were kept under stirring for 24 hours at room temperature and then centrifuged, and the solid product was washed and dried, as done for the plain nAp sample.
For the synthesis of iron-containing hydroxyapatite and in the presence of bio-based substances (nAp–Fe–BBS), 1.00 g of BBS and 5 g of Ca(OH)2 were added to 40 mL of water. The resulting suspension was left under stirring for 24 hours before starting the synthesis. 1.27 g of FeCl2·4H2O and 1.79 g of FeCl3·6H2O were added to a solution of 60 mL of water and 4.44 g of phosphoric acid and placed under stirring at 60 °C for 4 hours until complete dissolution of the salts. Subsequently, this solution was added dropwise to the suspension of Ca(OH)2 and BBS over 2 hours under stirring at a constant temperature of 80 °C and then kept for 24 hours at room temperature. After this step, the precipitate was separated from the supernatant by centrifugation, washed several times with deionized water, and finally dried in an oven at 80 °C for 24 hours. In Fig. 1a, images of the powders of the three samples are shown.
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Fig. 1 As-prepared nAp, nAp–Fe and nAp–Fe–BBS photographs (a) and preliminary characterization, N2 adsorption isotherms (b), BJH pore size distribution curves (c) and X-ray diffractograms (d). |
A portion of the samples was subjected to UV-C irradiation for 2 hours for sterilization purposes with a lamp emitting UV rays at 253.7 nm and an ultraviolet output of 13.4 W (Sankyo Denki, Japan). The indication “UV” was added to the labels of the so-treated samples.
Another treatment the samples underwent was calcination at 600 °C for 3 hours in a muffle furnace. In this case, the materials were identified as nAp 600, nAp–Fe 600 and nAp–Fe–BBS 600.
The diffractometer used for XRD was a PW3040/60 X'Pert PRO MPD X-ray diffractometer of PANalytical (Malvern, UK), with a Bragg Brentano geometry. Qualitative data processing took place with PANalytical X'pert High Score software and the diffractograms of the samples were compared with those provided in the ICDD-PDF-2 database. An evaluation of the crystalline domain was calculated employing the Scherrer equation relating the size of submicrometre particles in a solid to the broadening of diffraction pattern main peaks and considering k = 0.89 as the shape factor and λ = 0.154056 nm as the wavelength of the X-rays for Cu Kα1 radiation. Full width at half-maximum (FWHM) determination was performed with Origin23 software.
Nitrogen gas-volumetric adsorption experiments for textural property determination were performed at 77 K through an ASAP2020 (Micromeritics, Norcross, GA, USA). Before each measurement, the samples were outgassed overnight at 80 °C at a residual pressure of about 10−2 mbar to guarantee a good cleaning of the sample surface. The specific surface area of the samples was calculated by the Brunauer–Emmett–Teller (BET) model,35 whereas the total pore volume and the pore size distribution were evaluated by applying the Barrett–Joyner–Halenda (BJH) equation36 to the desorption branch of the isotherms, typically employed for mesoporous materials.
ζ-Potential measurements were carried out on a Zetasizer (Malvern Instrument, Malvern, UK). The ζ-potential values were measured using principles of laser Doppler velocimetry and phase analysis light scattering (M3-PALS technique). All the suspensions were prepared by dispersing 10 mg of powder in 20 mL of double distilled water. The pH values were adjusted in a range of 5.5–10 by adding the necessary volume of 0.1 M HCl or 0.1 M NaOH aqueous solutions. The analysis could not be conducted at pH values lower than 5.5, due to the higher solubility of the hydroxyapatite under such conditions.
The vibrational features of the materials were investigated through attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. The spectra were recorded with a Thermo Nicolet iZ10 spectrometer (Milan, Italy) equipped with a Smart Endurance TM (ZnSe crystal) in the range of 4000–650 cm−1 with 32 scans and 4 cm−1 band resolution.
The thermal behavior of the samples was investigated by thermogravimetric analysis (TGA). For these analyses (TGA 1 Star System of Mettler Toledo, Schwerzenbach, Switzerland), ca. 10–15 mg of samples were heated from 30 °C to 800 °C at a rate of 20 °C min−1 in a 70 mL min−1 N2 flow. The first derivative of TGA (DTG) was utilized to identify the temperature of maximum mass-loss rates.
Desorption tests of Al and Cr were conducted in ad hoc tests, after 1-hour adsorption. The suspension was, then, centrifuged and the solid was separated and mildly washed with MilliQ® water to remove the metal ions in excess on the surface (but not adsorbed). This material was left to dry. In the desorption process, the spent powder was suspended in acidified water (nitric acid, pH 5.5) for 1 hour, and the desorbed ions were quantified by ICP-OES.
Moreover, to understand the effect of the nanohydroxyapatite leaching, the release of Ca, Fe, Al, Ni and Cr ions was monitored (200 mg L−1 of each sample in MilliQ® water at a circumneutral pH). After 1 h stirring, the powders were separated by centrifugation and the amount of metal ions in the remaining supernatant was determined by ICP-OES.
Nanohydroxyapatite powders (200 mg L−1) were also used to adsorb 5 mg L−1 dye solutions (methyl orange, 85%, Sigma Aldrich and crystal violet, >90%, Sigma Aldrich) adjusted with NaOH to reach neutral pH. The dye bleaching was monitored using a double-beam UV-visible spectrophotometer CARY 100 SCAN (Varian, Palo Alto, CA, USA). A sample quartz cell of 1 cm path length was used. The maximum absorbance of the dyes was monitored to determine the adsorption percentage (464 nm for methyl orange and 590 nm for crystal violet). The adsorption isotherm at 25 °C was constructed using different concentrations of crystal violet (CV) in the range of 0–40 mg L−1 at pH 7, keeping the materials under stirring in the dye solution for 24 hours to ensure equilibration. The Langmuir model was used to describe the dye adsorption from the solution, whose equation is given below (eqn (1)):
![]() | (1) |
Crystal violet desorption tests were carried out by re-suspending 750 mg L−1 spent powder (after filtration and drying) in distilled water and acidified water (nitric acid, pH 5.5) for 1 hour and 24 hours.
The bacteria were grown in a suitable nutrient broth (buffered peptone water for microbiology, VWR Chemicals) for 24 h at 37 °C. The bacteria concentration was measured with a spectrophotometer (following the absorbance at 475 nm) and diluted into a sterile buffer to obtain a 1.5–3.0 × 105 CFU ml−1 working dilution. This bacterial inoculum was put in contact with the antibacterial agent under shaking at room temperature for 1 hour with a nanohydroxyapatite/inoculum ratio of 1 g:
50 ml. After this time, 1 ml of inoculum was diluted 1000 times and plated in Petri dishes with yeast extract agar (Sigma Aldrich). The Petri dishes were incubated for 24 hours at 37 °C and then, the surviving bacteria colonies were counted and compared to the initial bacteria concentration of the inoculum to calculate the % bacterial reduction using this equation (eqn (2)).
![]() | (2) |
The materials employed were nAp, nAp–Fe, nAp–Fe–BBS after UV sterilization and the calcined powders.
Sample | Specific surface area, BET (m2 g−1) | Cumulative pore volume, BJH (cm3 g−1) |
---|---|---|
nAp | 34 | 0.39 |
nAp–Fe | 83 | 0.41 |
nAp–Fe–BBS | 90 | 0.21 |
It is observed that the co-precipitation of iron oxide produced particles of reduced size, causing an increase in specific surface area from 34 to 83 m2 g−1. In parallel, the porosity of the samples increased as a result of the presence of interparticle spaces. By also adding the BBS, the surface area seems to have increased further, although not significantly (since the error to be attributed to the model is ±5%) and the total porosity has halved, indicating a system of particles denser than the previous one.
The XRD results for nAp, nAp–Fe and nAp–Fe–BBS in Fig. 1d resembled those reported elsewhere,20 confirming that the incorporation of iron and BBS did not modify the nanohydroxyapatite structure, except for the crystallite size, which resulted in smaller dimensions in the presence of Fe and/or BBS. In fact, as determined by the Scherrer method, applied to the principal reflection of hydroxyapatite, the 20–35 nm crystallites of nAp decreased in size by 30 up to 50% for nAp–Fe and nAp–Fe–BBS.
Such change is also confirmed by BET surface area results (increasing in the order n-Ap < n-Ap–Fe < n-Ap–Fe–BBS), suggesting that crystallite and particle sizes go hand in hand, thus excluding the presence of additional amorphous phases. Among the peaks at 2θ = 35.5°, 30.2° and 62.5° that have been previously attributed to the presence of iron oxides, herein, only the reflection at 35.5° as a single, independent peak is easily detectable. However, the formation of magnetite/maghemite can be found in the magnetic activity of the samples. The signals at 2θ = approximately 13°, 26.5° and 30° visible on the nAp sample are attributable to impurities of other phases of calcium phosphates (i.e., β-tricalcium phosphate and monetite),40,41 which are not found in the modified nanohydroxyapatites, probably since their formation is not favored in the presence of other substances (metallic or organic).
Regarding ζ-potential, it can be observed in Fig. 2 that from pH 5.5, all the samples presented a negatively charged surface that progressively became more negative, with both increasing the pH and adding the different doping compounds (magnetite/maghemite and BBS). This latter evidence is explicable by considering that magnetite/maghemite alone42–44 and BBS-containing materials follow similar trends; in particular, for BBS, this fact is attributable to the wide number of dissociable carboxylic groups present in their macromolecular structure.34
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Fig. 2 Zeta potential trend as a function of pH values for the different samples of nanohydroxyapatites. |
In the infrared spectra of Fig. 3, it is possible to distinguish the fundamental vibration modes and the related signals of the phosphate groups of the apatites (960 and 1022 cm−1), which are sharper for the nAp specimens (Fig. 3a). The residual carbonates derived from the synthesis process and the interaction with atmospheric CO2 are visible at 875, 1415 and 1455 cm−1 due to the substitution of PO43− by CO3−2,45–47 whereas the hydroxyls of physisorbed water typically present on the surface of solids fall under the signals centered at 1640 and 3430 cm−1 (bending and stretching, respectively).48 Only in the nAp spectrum, the peak at 3570 cm−1 was distinguishable (although weak), representing the structural OH ions of the hydroxyapatite lattice,48,49 which was previously correlated to a high crystallinity degree.20 For the Fe-modified nanohydroxyapatites (Fig. 3b), the main characteristic peaks of the iron oxides were not identifiable, since, given their low concentration, they could have been covered by the hydroxyapatite signals. Still, a higher presence of hydroxyl groups has increased the intensity of the bands around 3430 and 1640 cm−1. As already found for other magnetite-containing systems, this event may be ascribed to the formation of iron hydroxyl functional groups in the form of Fe(OH)2, Fe(OH)3 and FeO(OH).50 Following this trend, as a consequence of the addition of BBS (Fig. 3c), more intense absorptions could be found in the same zone, due to the OH groups of phenols, alcohols and carboxyl groups, as well as by the CO groups of carboxyls and carbonyls, abundantly present in BBS.22 Regarding the influence of post-synthesis treatments, on the one hand, the vibrational modes seemed not to be affected by UV exposure (photostability51). On the other hand, the calcination changed different spectral features. First, the region of OH suffered from a remarkable intensity decrease as a result of surface water desorption and, in the case of nAp–Fe–BBS, the combustion of organic substances. Moreover, by looking at the zoomed-in region in Fig. 3d, it is possible to make some considerations about the carbonate-related signals. As mentioned, signals at 875 (out-of-plane bending vibrations), 1415 and 1455 cm−1 (asymmetric stretching vibrations) indicate the substitution of carbonates for phosphate, which defines the B-type carbonates.52,53 Instead, when the substitution occurs at the expense of hydroxides, A-type carbonates are formed and are characterized by the shift of the peaks to 878, 1450 and 1545 cm−1; the relative A- and B-type presence in apatites is known to influence their structural and functional properties.52–55 After calcination at 600 °C, aided by the high temperature and by the fact that the hydroxyl group amount has been evidently reduced, the materials nAp–Fe and nAp–Fe–BBS were progressively transformed into AB-type carbonated hydroxyapatites.52,56,57 For nAp–Fe–BBS, this phenomenon could have been accentuated due to the availability of organic molecules that could have locally created a CO2-richer environment.
From Fig. 4, TGA outputs can be examined. First, the as-prepared and UV-subjected samples followed substantially the same trend and this was also verified by DTG peaks (data not shown), confirming the stability to the UV treatment, already seen in the FTIR outcomes. The mass loss related to adsorbed water (centered at 150 °C, according to DTG) for nAp and nAp UV was very limited (<1 wt%), whereas for modified nanohydroxyapatites, the value was comprised between 4 and 8 wt%. The dehydration of hydroxides (380–500 °C) and the elimination of surface carbonate-like groups (500–800 °C) were found to be the subsequent steps of thermal degradation and, for the BBS-added samples, organic matter pyrolysis occurred.20 The steeper phenomenon visible at about 440 °C for nAp(UV) has been correlated to the reaction of two molecules of calcium monohydrogen phosphate, which drove the formation of pyrophosphate and water.58 The DTG peaks present at 670 °C for nAp–Fe(UV) and 705 °C for nAp–Fe-BBS(UV) (broader), corresponding to changes in the slope in the TGA curves, were instead relatable to decarboxylation.58 The residues at 800 °C were approximately 5, 12 and 20 wt% for nAp(UV), nAp–Fe(UV) and nAp–Fe–BBS(UV), respectively. The BBS contribution to the TGA residue is due to the complex composition, which includes the presence of about 30% of inorganic compounds constituted by oxides and carbonates of Ca, Si, Fe, K, Na, Al, and Mg, plus other microelements.22
Regarding the calcined samples, the nAp 600 residue was lower than 2 wt%, while nAp–Fe 600 and nAp–Fe–BBS 600 reached 3.5 wt%. If the DTG of nAp 600 was very flat throughout the whole temperature range, for nAp–Fe 600 and nAp–Fe–BBS 600, the peaks related to carbonate decomposition were both centered at 690 °C. For the BBS-containing sample, the phenomenon was broader, probably indicating that multiple events were still subtended to the peak.
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Fig. 5 Adsorption 1 h kinetics for nAp, nAp–Fe, and nAp–Fe–BBS (200 mg L−1) in contact with solutions of Al(III), Cr(III), Ni(II) and As(V) (1 × 10−4 M) at pH 7. |
Regarding the desorption tests of Al and Cr, it was negligible, indicating a strong interaction with the nanohydroxyapatites, as already observed with the irreversible adsorption of Pb(II).20,21
Regarding the adsorption performances on dyes that have been tested here for the first time for these doped systems, methyl orange was not removed from the solution by any of the tested materials, due to the non-advantageous electrostatic interaction between the adsorbent negative surface and the methyl orange anionic molecule. Crystal violet (cationic), instead, was captured, but only by the nAp–Fe–BBS powder (Fig. 6a), reaching the 60% adsorption level in 1 hour and thus confirming the affinity of this dye with BBS.16,23,62Qmax was estimated through isotherm fitting with the Langmuir model (R2 = 0.92) to be 52 mg g−1 (see Fig. 6b), which is in line with the average results of other hydroxyapatite-based systems and, in general, other materials already described in the literature.63–66 Under the tested conditions, the CV desorption was limited to 1–2%.
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Fig. 6 (a) Adsorption 1 h kinetics for nAp–Fe–BBS (200 mg L−1) with 5 mg L−1 CV solutions; (b) isotherm at 25 °C for CV adsorption and fitting with the Langmuir model (red line). |
Sample | S. aureus bacterial reduction (%) | E. coli bacterial reduction (%) |
---|---|---|
nAp UV | 87 | 95 |
nAp–Fe UV | 91 | 0 |
nAp–Fe–BBS UV | 14 | 15 |
nAp 600 | 65 | 95 |
nAp–Fe 600 | 39 | 31 |
nAp–Fe–BBS 600 | 0 | 48 |
The antibacterial activities of nanohydroxyapatites have already been proven32 and confirmed herein against both the analyzed bacterial strains. The nano-size of the particles can be itself an important characteristic in destabilizing the bacterial cell membrane and enhancing the penetration ability.30,67,68 Moreover, positive charges (surface Ca2+)69 of nAp can react with the negative charges of the bacterial cell membrane, and also reactive oxygen species (ROS) able to inhibit the growth of bacteria can be formed, resulting from the optical properties of nAp that can capture visible light and generate hydroxyl radicals (·OH).32 Hariani et al.,30 who tested both Staphylococcus aureus and Escherichia coli, attributed the activity of a nanohydroxyapatite produced from snakehead fish bone to the action of the OH group, which can destroy the cell wall and the structure of bacterial proteins through different mechanisms. In the literature, antibacterial properties were also attributed to iron oxide nanoparticles.68
Looking at the results in Table 2 regarding the S. aureus decontamination process, BBS addition caused a significant decrease in antibacterial activity and, for all the materials, calcination negatively affected the performances.
The loss of antibacterial activity for the BBS-containing material could not be attributed only to a matter of surface charge change, which became significantly more negative than nAp (around −25 mV vs. −4 mV at pH 7, Fig. 2). Indeed, it is recognized that the higher the positive charges, the higher the efficiency in puncturing bacteria cell membranes can be obtained,70,71 but, in the present research, nAp–Fe showed a very similar zeta potential trend with respect to nAp–Fe–BBS, without losing its activity. One of the reasons on the basis of nAp–Fe–BBS UV behavior can be related to its microstructure, given the reduction of the crystalline domain and the formation of more agglomerated particles72 (this latter consideration is also valid for nAp 600, nAp–Fe 600 and nAp–Fe–BBS 600, considering the agglomeration as an effect of calcination20).
However, what also seems interesting to investigate is the modification of surface chemical reactivity. Actually, the increment in modified nAp of OH groups, detected by ATR-FTIR and previously by X-ray photoemission spectrometry,20 did not bring any positive effect, leading to the hypothesis of a more important role of calcium ions, which may have been, instead, firstly substituted by Fe2+/Fe3+ and then complexed by BBS, hindering their function. Similarly, the alteration of surface species can be the key to the activity diminishing after calcination. Based on the ATR-FTIR interpretation with the new arrangements of the carbonate species, it is worth taking into account that the exchange of phosphate ions (−3) with carbonate ions (−2) has been reported to lead to the creation of OH− and/or Ca2+ vacancies.31,73 The extent of this process may have been limited after calcination with the partial substitution of B-type with A-type carbonates, whose charge compensation involves the removal of two OH− ions per CO32− moiety.73 Moreover, if OH− ions could have had an effect on the antibacterial properties in nanohydroxyapatites, their lessening after heating was deleterious in the S. aureus reduction. Lastly, as anticipated, BBS, due to their inherent characteristics, brought about inorganic compounds, which, remaining as ashes after organic matter decomposition at 600 °C, could be another factor of property modification.
When the levels of E. coli reduction are examined (Table 2), the scenario changes. Except for nAp, which maintained excellent antimicrobial performance even after calcination, the overall situation seemed inverted: both nAp–Fe UV and nAp–Fe–BBS UV showed an irrelevant activity, whereas calcination enhanced the antibacterial features, although limited. This fact is attributable to the difference in the cell membranes between Gram-negative and Gram-positive bacteria and their susceptibility to the composition and active species of antibacterial agents.70 Briefly, Gram-negative bacteria cell walls are composed of thin peptidoglycan layers, surrounded by an outer membrane containing lipopolysaccharides. Gram-positive bacteria, instead, lack an outer membrane but are surrounded by layers of peptidoglycan that are significantly thicker than those present in Gram-negative bacteria.74 In the case of nAp–Fe UV and nAp–Fe–BBS UV, the most impactful characteristics preventing bacterial reduction could be the strongly negative surface charge that can cause intense charge repulsion and, although somehow contradictory with respect to some literature studies, the relevant presence of OH− species (indeed, nAp and nAp 600 samples with a low OH FTIR signal retained their antimicrobial properties).75 Furthermore, the already discussed substitution of carbonates at the expense of hydroxyls, as a consequence of calcination, resulted in being more convenient.
Albeit all these observations, it is not straightforward to correlate the net variation of antibacterial properties to a single specific parameter, but the importance of the presence and exposure of the ionic species (calcium, carbonates, phosphates, iron, hydroxyls) on the material surface clearly emerges, as previously reported.29,54,76,77 Peccati et al.54 studied the remarkable influence of carbonates on water adsorption features of hydroxyapatites by a computational approach. In another study, Ca- and P-deficiency and the incorporation of carbonate anions into phosphate sites for Ag-doped hydroxyapatites played a role in bactericidal properties.29
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