Mechanistic characterisation of a fungal fusicoccane-type diterpene synthase involved in the biosynthesis of talaro-7,13-diene

Fu-Long Lin; Kizerbo A. Taizoumbe; Yi-Xuan Wang; Jia-Hua Huang; Gao-Qian Wang; Guo-Dong Chen; Jian-Ming Lv; Dan Hu; Hao Gao; Jeroen S. Dickschat

doi:10.1039/D4OB01348D

Mechanistic characterisation of a fungal fusicoccane-type diterpene synthase involved in the biosynthesis of talaro-7,13-diene†

Fu-Long Lin,‡^a Kizerbo A. Taizoumbe,‡^b Yi-Xuan Wang,‡^a Jia-Hua Huang,^a Gao-Qian Wang,^a Guo-Dong Chen,

^a Jian-Ming Lv,*^a Dan Hu,

^a Hao Gao

^a and Jeroen S. Dickschat

*^b

Author affiliations

* Corresponding authors

^a Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou 510632, China
E-mail: ljm21@jnu.edu.cn

^b Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany
E-mail: dickschat@uni-bonn.de

Abstract

The cyclisation mechanism of the fungal fusicoccane (FC)-type diterpene synthase (DTS) TadA was investigated by extensive isotopic labelling experiments, and the pH-dependency of the product selectivity of this enzyme was explored. These studies provide new insights into the cyclisation mechanisms of FC-type DTSs.

Organic & Biomolecular Chemistry

Mechanistic characterisation of a fungal fusicoccane-type diterpene synthase involved in the biosynthesis of talaro-7,13-diene†

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Mechanistic characterisation of a fungal fusicoccane-type diterpene synthase involved in the biosynthesis of talaro-7,13-diene

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