Issue 48, 2024

Co-production of polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) by halophilic archaeon Haloferax mucosum

Abstract

This is the first report on the co-production of polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) by the halophilic archaeon Haloferax mucosum (DSM 27191) in a modified ATCC 2185 medium (NaCl 2.5 M, yeast extract 6 g L−1) with nitrogen limitation (C/N = 35 molar ratio), where it can produce 6.7 gEPS L−1 and 0.25 gPHA L−1. The PHA production is favoured when NaCl increases to 4 M and at higher nitrogen limitation (C/N = 50) reaches 0.75 gPHA L−1, but the EPSs were not detectable in the supernatant. The recovered PHAs are a copolymer of P(3HB-co-3HV), and the production conditions impact their chemical features (i.e. 3HV content, melting temperature, degree of crystallinity and molar mass). EPSs are anionic sulfated heteropolysaccharides composed of uronic acids, glucose, galactose, mannose and fructose. Purified EPSs have a high molar mass of 901 kDa and exhibit shear-thinning rheological behaviour in aqueous solution.

Graphical abstract: Co-production of polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) by halophilic archaeon Haloferax mucosum

Supplementary files

Article information

Article type
Paper
Submitted
24 May 2024
Accepted
08 Nov 2024
First published
25 Nov 2024

New J. Chem., 2024,48, 20188-20200

Co-production of polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) by halophilic archaeon Haloferax mucosum

M. A. López-Ortega, M. Escalante-Avilés, A. I. Rodríguez-Hernández, Ma. D. R. López-Cuellar, R. Y. Aguirre-Loredo, V. M. Martínez-Juárez, F. Pérez-Guevara, M. Á. Hernández-Valdepeña and N. Chavarría-Hernández, New J. Chem., 2024, 48, 20188 DOI: 10.1039/D4NJ02422B

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