Rebecca A.
Wilkes
ab,
Andrew J.
Borchert
ab,
Valentina E.
Garcia
cd,
Gina M.
Geiselman
cd,
Sarah
Liu
e,
Adam M.
Guss
bf,
Joshua K.
Michener
bf,
Daniel R.
Noguera
e,
Eiji
Masai
g,
John M.
Gladden
cd,
John
Ralph
e and
Gregg T.
Beckham
*ab
aRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. E-mail: gregg.beckham@nrel.gov
bCenter for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA
cJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA
dBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA 94550, USA
eGreat Lakes Bioenergy Research Center, University of Wisconsin–Madison, Madison, Wisconsin, USA
fBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA
gDepartment of Materials Science and Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
First published on 5th November 2024
Bioconversion of lignin-rich streams requires microbial hosts capable of utilizing and tolerating heterogeneous mixtures of monomeric and oligomeric compounds. Promising strains such as Novosphingobium aromaticivorans F199, N. aromaticivorans JMN2, Pseudomonas putida KT2440, Rhodococcus opacus PD630, Rhodosporidium toruloides NBRC0880, Sphingobium lignivorans B1D3A, and S. lignivorans SYK-6 possess inherent catabolic abilities to utilize lignin-related compounds (LRCs). In this work, we compared the cellular fitness and catabolic capabilities of these six bacteria and one yeast on a lignin-rich stream, alkaline pretreated liquor (APL) from corn stover, and on representative aromatic and aliphatic compounds. First, a minimal medium recipe that supported the growth of all seven strains on LRCs was selected for the comparison. Using this minimal medium, P. putida KT2440 was found to have the fastest growth and greatest tolerance when grown on guaiacyl-type compounds, p-hydroxyphenyl-type compounds, aliphatic acids, corn stover APL, and a model chemical mixture, whereas the S. lignivorans strains had the fastest growth on the syringyl-type compound. After 120 h on APL, the change in total lignin was 10–12% and aromatic and aliphatic compound usage was 85–96% for all the strains except R. opacus PD630, which had minimal utilization of APL components. Although substantial conversion of high-molecular-mass lignin was not observed by any strains, the S. lignivorans strains showed detectable modification β-ether units. Additionally, the N. aromaticivorans strains liberated aromatic compounds, potentially from lignin oligomer modification. This work serves as a comparison of seven promising microbial strains for bioconversion of lignin-enriched streams, providing a foundation for evaluating suitable microbial platforms for lignin valorization and genetic reservoirs to source unique metabolic capabilities.
Multiple putative microbial hosts have been identified and evaluated in self-consistent manners.17–20 Among the strains studied previously, Pseudomonas putida KT2440, Amycolatopsis sp. 39116, Rhodococcus jostii RHA1, Acinetobacter baylyi ADP1, and Corynebacterium glutamicum 534 were recognized for their catabolism of a large fraction of lignin-derived chemical constituents, tolerance to high concentrations of lignin feedstocks, and potential modification of high-molecular-mass (HMM) lignin.17–20 In particular, P. putida KT2440 has been well-studied as a chassis strain due to its rapid growth, comprehensive catabolic potential for varied substrates, native machinery for stress tolerance, genetic tractability, and ability to accumulate target products at high titers.21–24
As the field progresses, several other microbial strains have emerged as potential hosts that merit comparison for biocatalytic lignin valorization. Highlighted for metabolism of diverse aromatic compounds, these microbial host candidates include Sphingobium lignivorans,25,26Novosphingobium aromaticivorans,27Rhodococcus opacus PD630,28 and Rhodosporidium toruloides NBRC0880.29 In particular, S. lignivorans SYK-6 and N. aromaticivorans strains have been well-studied for catabolism of lignin-relevant dimers and their usage of syringyl (S)-type, guaiacyl (G)-type, and p-hydroxyphenyl (H)-type aromatic compounds.25,30,31 Additionally, R. opacus and R. toruloides have gained attention for their utilization of lignin-related compounds (LRCs) and production of lipid products at high yields.28,29,32 All of these microbial species can also be engineered to funnel LRCs to valuable products.33–37 In-depth characterizations of these microbes through systems biology and transposon insertion sequencing studies have furthered the understanding of the native enzymes involved in aromatic compound catabolism and metabolic reactions that support cellular growth and energy demands.30,31,38–43
In this work, we assessed the capacity of seven promising microorganisms to catabolize, tolerate, and modify components of lignin-enriched streams to evaluate their potential as hosts or genetic reservoirs for lignin valorization. We first evaluated different minimal media to establish a single medium that could support the growth of all seven microbes on LRCs. Next, we compared the metabolic capabilities of the strains on both model lignin stream compounds and a representative lignin-rich stream, corn-stover-derived alkaline pretreated liquor (APL). With several analytical techniques, we found that, apart from R. opacus PD630, which did not substantially utilize the APL components, the percent change in lignin was comparable between strains, but the rate and specificity of monomeric carbon utilization differed. Additionally, limited structural changes to the lignin substrate were measured with two-dimensional nuclear magnetic resonance (NMR) spectroscopic analysis. Overall, this work provides a direct comparison of the catabolic capabilities of several notable microbes for growth and bioconversion of lignin-rich streams. The findings of this work aim to provide a comparative analysis of the proposed hosts for lignin bioconversion to performance-advantaged bioproducts and recognize the potential use of different strains as genetic resources for unique metabolic capabilities.
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Fig. 1 Compatibility of minimal media to support growth on mock lignin stream. Strains were grown in several base media compositions that contained either 10% or 25% mock APL as a carbon and energy source, in which APL comprises a mixture of aliphatic acids (lactate, acetate, and glycolate) and aromatic acids (p-coumarate, ferulate, and vanillate). Maximum absolute growth rates and lag times were calculated using Gompertz fits of the growth dynamics illustrated in Fig. S1 and provided in ESI Excel File 1.† If no detection of growth occurred after 75 h, the strains were considered to not grow in that medium and mock-APL concentration. The data are the means of the maximum absolute growth rate or lag time measurements determined from three biological replicates and error bars denote the standard deviation of the mean values. Minimal media typically reported for growth of each strain are denoted with triangle symbols. Calculations for the averaged performance score are depicted in ESI Excel File 1.† |
The minimal media recipes examined included MM457, M9, RM, SD, and Wx that, respectively, have been used to grow JMN2,31 KT2440,17 PD630,44 NBRC0880,29 and SYK-645 (Table S1†). Additionally, K3 medium was included as it was previously utilized for co-cultures of Saccharomyces cerevisiae and Escherichia coli.46 To identify a medium that supports growth on lignin streams, a “mock APL” chemical mixture was utilized as the carbon and energy source, as described previously.47 This mock-APL mixture was intended to contain the most abundant aromatic and aliphatic compounds present in corn stover APL generated at 130 °C with 250 mg NaOH per g dry biomass.18,47,48 The composition of 100% mock-APL mixture was 30 mM p-coumarate, 20 mM ferulate, 6 mM vanillate, 44 mM acetate, 63 mM lactate, and 60 mM glycolate. To account for potential toxicity from high concentrations of aromatic or aliphatic acids, the 100% mock-APL mixture was diluted in each minimal medium (v/v) to create 10% and 25% mock APL. B1D3A did not grow on 25% mock APL in any media type after 75 h, indicating that higher concentrations of mock APL would likely be toxic to the growth of this strain (Fig. 1).
Only KT2440 was able to grow on all media containing 10% or 25% mock APL, indicating a robust fitness for varied nutritional conditions (Fig. 1). NBRC0880 and PD630 grew under all conditions except for, respectively, 25% mock APL in Wx minimal medium and 25% mock APL in SD minimal medium. However, S. lignivorans and N. aromaticivorans strains were more sensitive to media type and either did not grow or grew poorly in SD or K3 media at both mixture concentrations, with 1.5- to 4-fold increases in lag time and up to 4.5-fold decreases in maximum absolute growth rate (hereafter referred to simply as ‘growth rate’), compared to that on their typical growth medium (Fig. 1). Both N. aromaticivorans strains (F199 and JMN2) also did not grow within 75 h on 25% mock APL in M9 medium (Fig. 1). Interestingly, SD, K3, and M9 have the highest concentration of salt ions, suggesting that these media might interfere with the osmotic balance of SYK-6, B1D3A, JMN2, and F199 (Table S2; Fig. S2†).
Although MM457 and RM minimal media formulations could be used to support growth of all seven strains, KT2440 had up to a 3-fold reduction in growth rate on MM457 relative to M9 minimal medium, potentially due to the low iron and ammonium concentrations in this medium relative to the others (Fig. 1; Fig. S2; Table S2†). RM minimal medium, however, supported all the strains at comparable or, in some cases, better lag times and growth rates than their typical growth medium (Fig. 1). Further, a medium performance score was calculated for each media type, in which a higher score represents better growth performance, assessed as higher growth rates and shorter lag times across all strains tested, and in which higher relative growth rate and lower relative lag time values were weighted equally during analysis. Details regarding these calculations can be found in the Materials and Methods section and all calculations are available in ESI Excel File 1.† The medium performance scores confirmed that RM minimal medium supported the best growth outcomes for all strains, whether using a 10% mock APL mixture or a 25% mock APL solution (Fig. 1). Accordingly, RM minimal medium was chosen for all subsequent experiments.
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Fig. 2 Growth capacity and tolerance of microbial strains to chemical constituents of lignin streams. Maximum absolute growth rates and lag times were calculated using Gompertz fits of the measured change in optical density over time, which are provided in ESI Excel File 1.† The change in lag (Δlag) was calculated relative to growth on the lowest concentration of each substrate that each strain grew on. Bacterial growth in 96-well plates containing 200 μL of growth medium was monitored on a BioscreenC Pro instrument. For NBRC0880, which did not grow consistently in the BioscreenC Pro, growth in 48-well plates containing 1 mL of growth medium was monitored using a BioLector® automated growth curve analysis system. Strains were grown in RM minimal media supplemented with increasing concentrations of mock APL, aromatic acids, aldehydes, or aliphatic acids as sole carbon sources ranging from 5 mM to 100 mM. The starting concentration for acetate and lactate was 10 mM to provide enough carbon to support cellular growth. If no measurement of growth occurred after 120 h, the strains were considered to not grow on that substrate concentration. To support growth on p-coumarate, 4-hydroxybenzoate (4HB), 4-hydroxybenzaldehyde (4HBald), acetate, and lactate for both Sphingobium sp. strains, 130 μM methionine was supplemented in the media, due to a previously reported methionine auxotrophy when grown on a substrate lacking a methoxy groups.49 The data represent the mean of the calculated growth rates and Δlag times determined from three replicates. |
To solubilize and neutralize the model acidic compounds, media was titrated with NaOH, and as such, higher concentrations of the tested compounds correlate with a higher Na+ concentration in the growth medium. To delineate the impact of Na+ on the growth phenotypes, each strain was grown on a favorable carbon source without or with the addition of NaCl ranging from 75 mM to 500 mM (Fig. S3 and S4†). RM minimal medium does not initially contain Na+ in its recipe (Fig. S2b†)—an advantage of utilizing RM minimal medium when neutralizing with NaOH. When the media was supplemented with 500 mM NaCl, all seven strains grew with varying degrees of inhibition to growth rates and lags (Fig. S3 and S4†). Relative to no addition of NaCl, KT2440, PD630, and NBRC0880 had the highest inhibition to growth rate (50% decreased) whereas F199 and JMN2 had the largest increase in lag (up to 30 h) due to the addition of 500 mM NaCl (Fig. S3 and S4†). Nevertheless, although high concentrations of Na+ impacted growth, Na+ did not prevent growth for any of the strains at the concentrations relevant to the solubilization of acidic compounds.
Although growth on acidic compounds could be examined at 100 mM, the maximum concentration for vanillin and 4-hydroxybenzaldehyde (4HBald) solubilized in dimethylsulfoxide (DMSO) was 50 mM due to solubility constraints. For both aldehydes, DMSO was maintained at 1% (v/v) in the media, which was at a concentration that did not impact growth for any of the strains (Fig. S3 and S4†). Generally, vanillin was less toxic than 4HBald as was demonstrated by six out of seven strains growing on 15 mM vanillin whereas 10 mM was the maximum concentration any of the strains grew on 4HBald (Fig. 2). However, NBRC0880 did not grow on vanillin at any concentration, indicating vanillin is not a favorable sole carbon source for this strain (Fig. 2). Overall, only KT2440 and B1D3A grew on 25 mM vanillin and only KT2440, B1D3A, and SYK-6 grew on 10 mM 4HBald (Fig. 2), highlighting the general toxicity of aldehydes to microbial growth.
The growth trends for strains from the same genus and species, S. lignivorans SYK-6 and B1D3A and N. aromaticivorans JMN2 and F199, were similar across the tested substrates with a few key differences: SYK-6 tolerated a higher concentration of mock APL than B1D3A, but B1D3A grew on higher concentrations of 4-hydroxybenzoate (4HB), vanillin, and acetate than SYK-6. JMN2 had a higher growth rate than F199 on lactate, but F199 grew on 100 mM acetate whereas JMN2 did not (Fig. 2). Larger differences in growth phenotype were observed when comparing across genera.
For all the microbial strains fed mock APL, only KT2440 and NBRC0880 grew on 100% of the mock APL whereas the rest of the strains only tolerated 50% mock APL or less (Fig. 2). Further, there was only about a 5 h increase in lag time between the 10% and 100% mock APL concentrations for KT2440 and NBRC0880, indicating high inherent tolerance to the aliphatic and aromatic compound mixture (Fig. 2). During growth on the individual substrates, KT2440 exhibited the highest tolerance (growth maintained up to 100 mM of all the acidic compounds tested, excluding syringate), shortest change in lag time (averaging Δ14 h between 5 mM and 100 mM), and fastest growth rates (on average around 0.5 h−1) in both 200 μL and 1 mL growth conditions as compared to the other strains (Fig. 2; Fig. S5†). Although the lag in growth remained consistent between growth in 1 mL and 200 μL for KT2440, the growth rates differed, likely due to aeration differences between culture conditions (Fig. S5†). Regardless of culture volume, KT2440 grew on all the acidic compounds up to 100 mM, excluding syringate (Fig. S5†).
Broad substrate catabolism and tolerance was also observed for PD630, which grew on up to 100 mM of the aliphatic acids, 4HB, and ferulate and up to 25 mM of vanillate and p-coumarate (Fig. 2). As substrate concentrations increased, PD630 exhibited up to 50 h increases in lag times, indicating a sensitivity to high concentrations of aromatic and aliphatic acids solubilized with NaOH (Fig. 2). In contrast, as the substrate concentration increased, NBRC0880 maintained consistent growth rates (with a max standard deviation of 0.01 h−1 between 5 and 100 mM) and lag times (typically ranging between 1–30 h) on mock APL, H-type aromatic compounds, and acetate (Fig. 2; ESI Excel File 1†). However, NBRC0880 had 40–90 h lag times on G-type aromatic compounds, grew only on 25 mM vanillate and 25 to 75 mM ferulate, and did not grow on vanillin (Fig. 2). Notably, vanillate was especially toxic for all the strains, as only KT2440 grew at and above 50 mM and at 25 mM the other bacterial strains had about an 80 h increase in lag time compared to at 5 mM (Fig. 2).
Syringate did not support the growth of KT2440, PD630, or NBRC0880 as a sole carbon and energy source, but S. lignivorans and N. aromaticivorans strains were able to grow without an additional substrate (Fig. 2). The two S. lignivorans strains (B1D3A and SYK-6) performed better than the N. aromaticivorans strains (F199 and JMN2) on syringate, growing at 25 mM and exhibiting 2-fold faster growth rates at 5 mM than JMN2 or F199 (Fig. 2). In contrast, both the S. lignivorans and N. aromaticivorans strains did not grow at or above 25 mM on p-coumarate or ferulate, indicating that they have a low native tolerance to the hydroxycinnamic acids, solubilized with NaOH, that are the most prominent monomeric aromatic components in corn stover APL.17,18 Overall, KT2440, followed by PD630 and NBRC0880, showed the broadest substrate tolerance and catabolic capacity demonstrated by growth on the chemical mixture, G-type compounds, H-type compounds, and aliphatic acids tested, but the two S. lignivorans strains (B1D3A and SYK-6), followed by the two N. aromaticivorans strains (F199 and JMN2), performed the best on the S-type compound.
All seven strains grew, as measured by an increase in optical density, on 25% APL in RM minimal medium as a sole carbon source (Fig. 3). Similar to growth trends on mock APL, KT2440 grew the fastest of the microbial strains on 25% APL, reaching stationary phase within 6 h. The next three fastest were SYK-6, PD630, and NBRC0880, which entered stationary phase within 12 h. For B1D3A and JMN2, stationary phase was reached by 24 h, whereas the slowest growth was found for F199, which required 48 h to reach a maximum OD600 value (Fig. 3). After reaching stationary phase, the microbial biomass generally remained constant without a decline in OD600, except for KT2440, which lost about 50% of its max OD600 at 24 h before mostly recovering by 96 h. The highest maximum OD600 values were observed for NBRC0880 and the two N. aromaticivorans strains (F199 and JMN2). After 120 h of inoculation, the cells were pelleted, and the cell-free supernatant was harvested for analysis of the APL.
To quantify the decrease in lignin content after microbial treatment, Klason lignin analysis of both acid-soluble and acid-insoluble fractions was conducted on the cell-free lyophilized APL and the percent change in lignin content, including both HMM and monomeric aromatic compounds, was calculated as described previously.17,52 Before microbial treatment, the majority of the lignin content (about 91%) was HMM or uncharacterized lignin components, whereas the quantified free H-type, G-type, and S-type aromatic compounds consisted of only 9.08 ± 0.22 g/g % of the total lignin content (Fig. 4a). After 120 h of microbial treatment, the maximum decrease in lignin content was 12.9 ± 1.3% in B1D3A (Fig. 4b; Fig. S6†). However, all the other strains, except PD630, reached comparable decreases in lignin content within the biological and instrumental error (Fig. 4b). Notably, PD630 did not significantly alter the total lignin content, the measured aliphatic and aromatic compounds, or the pH of the media, as compared to the abiotic control (Fig. 4b and c; Fig. S6–S8†). In contrast, the other six strains depleted the measured compounds by 85 to 96 mol C/mol C % and the pH of the media increased from 7.1 to ∼8.0 after 120 h (Fig. 4c; Fig. S7 and S8†). The highest significant percent decrease (mol C/mol C) in quantified aromatic and aliphatic acids was found for the two N. aromaticivorans strains, KT2440, and B1D3A (Fig. 4c and d). Although the overall depletion of free compounds, in mol C, was close to 100% after 120 h, there were key differences in the rate of substrate depletion and types of substrates used.
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Fig. 4 Microbial strain performance with 25% APL. (A) The composition of measured aromatic compounds in the lignin fraction of the APL for the abiotic controls after 120 h of incubation. (B) Percent decrease in lignin content representing both microbial lignin depolymerization and catabolism after 120 h of incubation with the seven strains or no inoculum (abiotic control). The dotted line indicates the percentage of measured aromatic compounds in the total lignin fraction of APL for the abiotic control. The measured lignin content in g L−1 from Klason analysis is depicted in Fig. S6.† (C) The percent decrease in the sum of all measurable residual carbon, both aromatic and aliphatic compounds, after 120 h of microbial treatment. The total mM of carbon remaining for each condition is illustrated in Fig. S7.† For A, B, and C, the bar graphs show the mean ± the standard deviation of three replicates. For B and C, statistically significant differences (p < 0.05) are denoted by a change in letter; significance was determined by one-way ANOVA followed by Tukey HSD post hoc tests. Exact p-values can be found in ESI Excel File 1.† (D) The conversion of the individual lignin-derived and carbohydrate-derived components of APL at different timepoints of growth relative to the initial content in the media (% remaining). The data represent the mean of three biological replicates. The measured concentrations, in mM, of all compounds for each strain and timepoint (6 h, 12 h, 24 h, 48 h, 72 h, and 120 h) are provided in ESI Excel File 1.† Compound names for 4-hydroxybenzoate and 4-hydroxybenzaldehyde were abbreviated as 4HB and 4HBald respectively. The compounds 4HB, vanillate, syringate, and acetovanillone were present in the initial media but were below the limit of quantification (LOQ). Due to evaporation over time, some compounds were able to be measured in the abiotic control and in some of the microbial treatments after 120 h. For 4HB, vanillate, syringate, and acetovanillone, if the difference between the detection of a compound in a microbial treatment versus the abiotic control at a specific timepoint was less than 0.01, the condition was left grey. |
As expected from the rapid growth on APL, KT2440 utilized all the measured compounds in APL, except oxalate and acetosyringone, within 6 h, which was, compared to the other strains, the most amount of carbon at the fastest rate (Fig. 4d; Fig. S9; ESI Excel File 1†). None of the strains utilized oxalate and the concentration increased over time (Fig. 4d). Both S. lignivorans strains depleted acetosyringone within 12 h (Fig. 4d), indicating it was, as expected, a viable carbon source for S. lignivorans.53 Although JMN2 and F199 did not completely deplete acetosyringone from the extracellular medium, the concentration after 120 h was 50% lower than in the abiotic control, indicating that these strains can convert this carbon source to some extent.
Comparable to the growth trends when acetate or lactate were sole carbon and energy source (Fig. 2), B1D3A depleted acetate 96 h before SYK-6 and neither S. lignivorans strain consumed lactate in the APL mixture (Fig. 4d). In fact, only KT2440 completely depleted lactate after 120 h. Reflecting the slower time to stationary phase, F199 depleted APL components 24–48 h slower than the other strains but, similarly to JMN2 and the S. lignivorans strains, the aromatic compounds were depleted before the aliphatic compounds (Fig. 4d; Fig. S9†). Of the tested microbial strains, NBRC0880 was the most discriminating in its substrate usage. After 12 h, NBRC0880 completely depleted five carbon sources but, even after 120 h, the strain did not utilize lactate, formate, oxalate, or acetosyringone (Fig. 4d). Some compounds, including acetovanillone, vanillate, and 4HB, appeared at 12 or 24 h in the inoculations with the two Novosphingobium strains (F199 and JMN2) but not in the abiotic control, implying potential modification of unmeasured aromatic-containing substrates in the APL, and/or secretion of these metabolites during the catabolism of upstream aromatic compounds (Fig. 4d).
Although the strains significantly utilized the free aromatic and aliphatic acids, the majority of APL consists of HMM components (Fig. 5). After 120 h of treatment, gel-permeation chromatography further illustrated a substantial decrease in low-molecular-mass (LMM) compounds, but also indicated changes in HMM peaks compared to the abiotic control for all the strains except PD630 and NBRC0880 (Fig. 5). The LMM peak closely matched the spectra of p-coumarate (Fig. S10†), which was also the aromatic compound found at the highest concentration in the APL (Fig. 4d). Unique from the other strains, both N. aromaticivorans strains also had a new LMM peak appear in the spectra (Fig. 5). However, only B1D3A significantly depleted the lignin content (p = 0.012) compared to the total quantified free aromatic compounds (ESI Excel File 1†), suggesting that only the monomer fraction of APL was appreciably depleted by most strains. To assess if the strains acted on the functional linkages in HMM lignin, NMR analysis was applied to the APL after 120 h of microbial treatment.
Although it is difficult to match correlation intensities across spectra, both KT2440 (Fig. 6a) and SYK-6 (Fig. 6b) depleted the p-coumarate compared to the abiotic control (Fig. 6c). The changes to the APL fraction after treatment with the microbes is most readily seen via the difference spectra between microbial treatment and the abiotic control (Fig. 6d–j). Volume-integration of the contours further delineated the major changes, showing the remaining p-coumarate at 13% and 20% of the original level, respectively, with all microbes except PD630 showing similar reductions (Fig. 6d–j). The only other notable difference between microbial treatment and the abiotic control was that SYK-6 and B1D3A exhibited mild depletions of β-ether units (Fig. 6e and f), presumably from metabolism of small β-ether-containing oligomers in the APL. The other strains did not appear to display a similar metabolism of such units despite the potential for β-etherase activity (Fig. 6d–j).25,58,59 Furthermore, there was no measurable difference in any of the other lignin units. However, additional non-lignin-related peaks in the APL medium showed a decrease in signal intensity especially for the S. lignivorans strains, the N. aromaticivorans strains, and NBRC0880 (Fig. 6d–j), indicating that non-lignin components were modified and potentially catabolized by these strains.
The broadest tolerance, as measured by growth performance metrics in batch culture using solubilized aromatic and aliphatic compounds, was observed for KT2440, PD630, and NBRC0880 (Fig. 1 and 2). Both P. putida and R. toruloides strains have been highlighted previously for their toxicity tolerance to a broad range of compounds.47,62–65 Here, this native tolerance was also illustrated, in general, by small changes in lag time (around a 15 h increase) between 5 mM and 100 mM of individual aromatic and aliphatic acids (Fig. 2). Aldehydes, important components of diverse lignin streams,17,66–68 were found to be especially toxic to microbial growth and only KT2440 and B1D3A grew on 10 mM 4HBald and 25 mM vanillin (Fig. 2). It is possible that different preculture strategies, such as an initial growth in minimal media instead of rich media, could improve growth outcomes and tolerance for all the microbial strains. Additionally, although the strains grew when exposed to 500 mM NaCl (Fig. S3 and S4†), the growth performance was inhibited on a favorable carbon source at high Na+ concentrations and thus there could be a combinatorial toxicity effect from Na+ and acidic compounds that prevented growth for some strains. Production strains could also have reduced tolerance compared to native strains, such as engineered N. aromaticivorans grown in standard mineral base medium that had a greater inhibition to Na+ supplementation than observed here.37 For all strains examined, additional strain-based optimization could be employed to improve host strain performance such as altering the neutralization reagents (e.g., NH4OH instead of NaOH),37 optimizing the growth medium, increasing the aeration through growth in bioreactors, as well as feeding strategies to slowly introduce aromatic or aliphatic compounds.
To accommodate all the strains in this work, a moderate concentration of corn stover APL (25% v/v) was chosen because growth of microbial strains was commonly impeded on NaOH-solubilized aromatic acid concentrations at or above 25 mM (Fig. 2). All strains, except PD630, grew in the corn stover APL medium (Fig. 3), indicating the presence of a sufficient amount of available carbon sources to support growth. Despite catabolism and tolerance to 50% mock APL (Fig. 2), PD630 did not significantly utilize components of the corn stover APL (Fig. 4d). This growth limitation could be due to an unmeasured inhibitory component in the corn stover APL. Since PD630 has been grown on other lignin streams with success, including kraft lignin and an alkali/alkali-peroxide-pretreated corn stover waste streams,32,44 the inhibitory components of this corn stover APL may not be present in other lignin streams. Supplementation of lignin streams with alternative carbon sources such as glucose can also be used to mitigate growth limitations of host strains.17,32,69,70 Efficient product formation will ultimately rely on feeding high concentrations of LRCs, which will subsequently increase inhibitory components, further emphasizing the need for broad detoxification and/or tolerance mechanisms in the host strain.
Another key factor in host selection is innate catabolic capabilities to rapidly utilize the heterogeneous compounds found in lignin streams, and thus minimize downstream needs to genetically insert novel metabolic pathways. In this study, all strains except PD630 consumed the two most abundant aromatic species, p-coumarate and ferulate, and the most abundant primarily carbohydrate-derived species, acetate, in the APL (Fig. 4d). The broadest free compound usage was found for the two N. aromaticivorans strains (F199 and JMN2), KT2440, and B1D3A, which all utilized between 93 and 96 mol C/mol C % of the quantified aromatic and aliphatic carbon in the APL (Fig. 4c and d). Exhibiting specialist capabilities, only the S. lignivorans strains completely depleted acetosyringone (Fig. 4d), which represented a small fraction of this corn stover APL at 0.04 mM but is an important compound common in other lignin streams.11,67,71,72 Catabolic pathways for acetosyringone have been identified in SYK-653 and could represent important genetic sources for the expansion of substrate usage in other hosts. In addition, pathways for degradation of other S-type aromatic compounds, such as syringate examined here, have been described for both S. lignivorans and N. aromaticivorans.33,51,69,73–75 While KT2440, PD630, and NBRC0880 did not utilize syringate as a sole carbon source, there is the potential for co-metabolism of S-type compounds by these strains as was demonstrated for KT2440 which utilized syringate when an additional carbon source such as vanillate or glucose was present.50,51 Although an ideal host strain for bioconversion of complex feedstocks should natively utilize the highest percentage of carbon, advances in synthetic biology and adaptive laboratory evolution have simplified approaches for substrate expansion and inhibitor tolerance. Future expansion of metabolic pathways in these strains could alter their capabilities.
Metabolism of lignin stream constituents can result in the appearance of compounds either through the accumulation of an intermediate in a metabolic pathway or from modification of oligomeric substrates. Accumulation of aromatic compounds occurred in the media at 12 and 24 h during the inoculations with both N. aromaticivorans strains. For both strains, 4HB and vanillate, known intermediates in the catabolism of p-coumarate and ferulate,39 appeared in the supernatant, potentially due to secretion of these compounds during the metabolism of the hydroxycinnamates. JMN2 was the only strain to have acetovanillone appear in the supernatant, which increased up to 0.08 mM after 120 h, suggesting that it was a product from modification of an uncharacterized lignin-derived species. Concurrently, the concentration of acetosyringone decreased by 50% in the JMN2 inoculation compared to the abiotic control (Fig. 4d). In SYK-6, the acetosyringone catabolism pathway can also act on acetovanillone.53 Previously, N. aromaticivorans PDC (derived from F199) was found to not utilize acetosyringone or acetovanillone in the presence of glucose,69 but supplementation with an aromatic compound may initiate metabolism, as demonstrated when vanillate supplementation enabled syringate metabolism in KT2440.76,77 Additional examination of the acetovanillone/acetosyringone metabolism in JMN2 is warranted to assess its catabolic capacity.
Lignin is a complex aromatic polymer containing structural inter-unit linkages, characterized by the bonds formed during the radical coupling steps in lignification, such as β-O-4, β-5, β-β, β-1, and 5-5. Enzymatic cleavage of representative dimeric compounds containing these linkages has been well-characterized in S. lignivorans SYK-6 and N. aromaticivorans strains.25,30,31,78,79 However, this work commonly uses model dimeric compounds instead of more complex oligomers like those in the HMM lignin fraction of APL. NMR indicated significant metabolism of p-coumarate but little to no changes in the structural units of the lignin backbone of the oligomers present in the APL, except for a decrease in the β-ether level observed after treatment with both S. lignivorans strains. Previously, etherases from SYK-6 recombinantly expressed in E. coli were shown to cleave β-ether linkages in a synthetically constructed lignin.80 Here, we found that both SYK-6 and B1D3A could also moderately deplete these linkages in corn-stover APL. The minimal conversion of HMM species in the APL stream could therefore represent a disconnect between the enzymes capable of cleaving dimers and activity on larger oligomers, whether due to spatial separation (i.e., intracellular enzyme and extracellular substrate), steric hindrance from more complex oligomers, lack of enzyme upregulation in the presence of APL, alterations to the lignin bioavailability due to high salt content, or inhibitors of enzyme activity in complex media. Of note, extracellular enzymatic lignin deconstruction through the action of oxidases, such as laccases and peroxidases, has shown potential to deconstruct lignin.81–83 Supplementation with exogenous laccases was found to act synergistically with bacterial catabolism by deconstructing lignin and improving the growth of PD630 on kraft lignin.44 Similarly, the growth of KT2440 on corn stover lignin solids was increased when a fungal secretome was added to act on HMM lignin.84 Improvements in enzymatic deconstruction of lignin remain an area of interest, but currently many challenges remain for it to become applicable at a commercial scale.81,85 For bioconversion to occur under process-relevant conditions, systematic improvements in obtaining a higher percentage of monomeric content from lignin is crucial.1,9,86
The chemical composition of lignin streams for bioconversion is dependent on both the plant species and the chemical deconstruction method. In this work, we utilized corn stover APL as a representative lignin stream due to its precedent in previous work.17,19,20 However, alkaline pretreatment represents only one of many approaches to extract lignin from biomass, and is effective only for grasses or for the few hardwood species (polar, willow, palms) that have p-hydroxybenzoylated lignins.11,85,87,88 In particular, catalytic oxidation offers a promising strategy for improving the yield of bioavailable, water-soluble aromatic compounds from lignin.1,66,68,89 The cleavage of C–O bonds in the oxidation processes is well known and can occur at near-theoretical yields, providing 20–30 wt% monomers based on the lignin content of intact biomass.66,68,89 Beyond the monomers available from aryl-ether bond cleavage in lignin, several oxidation studies have demonstrated C–C bond cleavage in lignin that could potentially increase the yields of aromatic monomers for bioconversion.90–92 Recently, the combination of C–O and C–C bond cleavage in an oxidative process enabled a yield of 28% lignin monomers from pine that could be funneled to muconic acid by P. putida.92 In another example, optimizing the catalyst and process conditions in reductive catalytic fractionation to produce aryl-propanols instead of aryl-propanes improved production of 2-pyrone-4,6-dicarboxylic acid by N. aromaticivorans.69 As catalytic deconstruction strategies further improve the bioavailable content of lignin streams, metabolic pathways to catabolize S-type lignin and dimers will become increasingly important. Host strain tolerance to high aromatic content and to the lignin stream matrix will also become essential traits for bioconversion.
The strains tested here utilize either the ortho-cleavage pathway (KT2440, PD630, NBRC0880) or the 4,5-meta-cleavage pathway (SYK-6, B1D3A, JMN2, F199) from the central aromatic pathway intermediate, protocatechuate.23,38–40,42,51 Performance-advantaged products have been obtained from these cleavage pathways through the deletion of enzymes downstream of the metabolite intermediate of interest combined with supplementation of an alternative carbon source to support biomass demands.1,23,23,34,37,60 For additional products that cycle through central carbon metabolism, the 4,5-meta-cleavage pathway has the advantage over the ortho-cleavage pathway due to both the formation of NADPH as well as total carbon conversion to central carbon metabolites (i.e., no carbon loss to CO2). When selecting a microbial host for bioconversion, it is important to consider the feedstock, deconstruction method, product of interest, catabolic and synthesis pathways, cofactor balance, and additional traits such as the ability to natively generate desirable products in large quantities. Going forward, the comparisons presented in this study can be employed as guiding information to select and improve microbial hosts for the bioconversion of lignin.
Microbial growth experiments on 25% APL in RM minimal medium were performed in 250 mL baffled shake-flasks containing 50 mL of culture medium and shaken at 225 rpm for 120 h. An initial aliquot of medium prior to the inoculation was collected and frozen at −20 °C until additional processing was conducted. Growth and extracellular samples were acquired after 6 h, 12 h, and then daily for 120 h. When strains were grown on APL, which is dark brown in color, cell suspensions were measured spectrophotometrically at OD600 with a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific) before aliquots were centrifuged at 5000g for 10 min and the cell-free supernatant for each aliquot was measured again at OD600, which was used to correct the original biomass measurement for the APL background coloration. The cell-free supernatant was further filtered through 0.22 μm nylon Costar® Spin-X® centrifuge tube filters (Corning) to ensure bacterial removal before analysis of the aliphatic and aromatic acid composition. After cultivation ended at 120 h, 35 mL of each culture were centrifuged at 4500g and 4 °C for 20 min to remove the cells, the supernatant was lyophilized, and the resulting freeze-dried APL was weighed and aliquoted for Klason lignin, GPC, and 2D NMR analyses.
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This work was authored in part by the National Renewable Energy Laboratory, operated by Alliance for Sustainable Energy, LLC, for the U.S. Department of Energy (DOE) under Contract No. DE-AC36-08GO28308. This work was also authored in part by Oak Ridge National Laboratory, managed by UT-Battelle, LLC for the US DOE under Contract Number DE-AC05-00OR22725. This material is based upon work supported by the Center for Bioenergy Innovation (CBI), U.S. Department of Energy, Office of Science, Biological and Environmental Research Program under Award Number ERKP886. DRN, SL, and JR were supported by the Great Lakes Bioenergy Research Center, U.S. Department of Energy, Office of Science, Biological and Environmental Research Program under Award Number DE-SC0018409. This work was part of the DOE Joint BioEnergy Institute (https://www.jbei.org) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy. Additional funding was provided by the U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Bioenergy Technologies Office, which additionally supported the analytical analysis of aromatic and aliphatic acids. The views expressed in the article do not necessarily represent the views of the DOE or the U.S. Government. The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this work, or allow others to do so, for U.S. Government purposes.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4gc03876b |
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