A systematic study on the effect of protonation and deuteration on electron spin Tm/T2 in a cellular context

Francesco Torricella; Valentina Vitali; Lucia Banci

doi:10.1039/D4CP00599F

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A systematic study on the effect of protonation and deuteration on electron spin T_m/T₂ in a cellular context†

Francesco Torricella,^ab Valentina Vitali^ad and Lucia Banci

*^acd

Author affiliations

* Corresponding authors

^a Magnetic Resonance Center, Università degli Studi di Firenze, 50019 Sesto Fiorentino, Italy
E-mail: banci@cerm.unifi.it

^b Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Health, Bethesda, Maryland 20892-0520, USA

^c Consorzio Interuniversitario Risonanza Magnetiche di Metallo Proteine, 50019 Sesto Fiorentino, Italy

^d Dipartimento di Chimica, Università degli Studi di Firenze, 50019 Sesto Fiorentino, Italy

Abstract

In recent years, DEER experiments in pulsed EPR have garnered interest for their precise distance distribution insights in cellular and buffer setups. These measurements linked to electron spin T_m/T₂ values of the labelled sample are impacted by the cellular environment being fully protonated or deuterated, as demonstrated in the present study.

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Article information

DOI: https://doi.org/10.1039/D4CP00599F
Article type: Communication
Submitted: 09 Feb 2024
Accepted: 10 Jul 2024
First published: 11 Jul 2024

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Phys. Chem. Chem. Phys., 2024,26, 20246-20250

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A systematic study on the effect of protonation and deuteration on electron spin T_m/T₂ in a cellular context

F. Torricella, V. Vitali and L. Banci, Phys. Chem. Chem. Phys., 2024, 26, 20246 DOI: 10.1039/D4CP00599F

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