Photoinduced adsorption of oligonucleotides on polyvinyl chloride films containing malachite green derivative

Abstract

DNA adsorption on the micrometer scale in a simple and cost-effective manner has received considerable interest. We prepared a film by casting an organic solvent containing polyvinyl chloride and a malachite green derivative, which can be photoionized to afford a cationic moiety for interaction with DNA. In this article, we report photoinduced oligonucleotide adsorption on a film that offers spatial and temporal control over oligonucleotide adsorption. Fluorescence microscopy was used to observe the oligonucleotide adsorption. Oligonucleotides of various sequences and lengths were also examined. UV irradiation using a photomask having 100 μm-diameter holes promoted the oligonucleotide adsorption on the film, whereas there was hardly any oligonucleotide adsorbed on the non-irradiated area. We found that the nucleobase contributed to the adsorption and part of the anchor in the oligonucleotide chain was responsible for the adsorption on the film.