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Correction: The influence of electron utilization pathways on photosystem I photochemistry in Synechocystis sp. PCC 6803
Sharon L. Smolinski,
Carolyn E. Lubner,
Zhanjun Guo,
Jacob H. Artz,
Katherine A. Brown,
David W. Mulder and
Paul W. King*
National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA. E-mail: paul.king@nrel.gov
First published on 3rd January 2023
Abstract
Correction for ‘The influence of electron utilization pathways on photosystem I photochemistry in Synechocystis sp. PCC 6803’ by Sharon L. Smolinski et al., RSC Adv., 2022, 12, 14655–14664, https://doi.org/10.1039/d2ra01295b.
The authors regret that in the Experimental section on Page 14657, there are six instances where the abbreviations of micromolar (μM), microliter (μl) and microgram (μg) are incorrectly noted as “mM”, “ml” and “mg”. The corrected units are given below:
Section 2.6. Fluorescence emission analysis
Fractions containing PSI trimers and monomers that were isolated using anion-exchange chromatography were normalized to 3.0 μg chl per ml and were measured at 77 K to quantify P700. Fractions containing PSI monomers and trimers that were isolated using sucrose gradients were normalized to 16 μg chl per ml and were measured at room temperature and 77 K to determine spectral properties.
Section 2.7. P700 spectroscopic analysis
P700 spectroscopic analysis on isolated fractions containing PSI monomers or trimers were normalized to equivalent amounts of P700 (0.84 μmol) and measured using 720 nm actinic light. Samples (2 ml) were placed in a quartz cuvette containing P700, 10 mM sodium ascorbate, and 10 μM 2,6-dichlorophenolindophenol (DCPIP) in 20 mM HEPES–NaOH, pH 7.5, with 10 mM CaCl2, 10 mM MgCl2, 10 mM NaCl, and 0.04% DDM.
2.8. Flavodoxin photoreduction assays
In order to determine the capacity of PSI monomers and trimers to transfer electrons out of PSI, equivalent amounts of P700 (44 nmol) were added to a quartz cuvette containing 10 mM sodium ascorbate, 30 μM phenazine methosulfate, and 100 μM flavodoxin (Fld), in 20 mM HEPES–NaOH, pH 7.5, with 10 mM CaCl2, 10 mM MgCl2, 10 mM NaCl, and 0.04% DDM, at a final volume of 350 μl, similar to ref. 26.
The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.
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