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Correction: Dietary L-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model
Mengdie
Chen
ab,
Yuyu
Liu
ab,
Shanbai
Xiong
ab,
Moucheng
Wu
a,
Bin
Li
ab,
Zheng
Ruan
*c and
Xiaobo
Hu
*ab
aCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. E-mail: foodnutrition314@126.com
bKey Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
cState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China. E-mail: ruanzheng@ncu.edu.cn
First published on 18th August 2023
Abstract
Correction for ‘Dietary L-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model’ by Mengdie Chen et al., Food Funct., 2019, 10, 2390–2398, https://doi.org/10.1039/C9FO00123A.
In the original article, Fig. 5 was not clearly presented. The authors apologise for the lack of clarity in the original figure and a new Fig. 5 is presented below to aid understanding.
The relevant changes to the citations in the section 3.5 text are as follows: 3.5 Dietary L-Trp prevents the LPS-induced down regulated expression of tight junction protein Claudin-1, Occludin and ZO-1.
When the monolayer was previously disrupted by LPS, dietary L-Trp at high concentrations (80 μM) significantly alleviated the decrease in claudin-1 (P < 0.05), with no significant differences between other concentrations in the PC group. Western blot analysis confirmed that dietary L-Trp could not improve the levels of tight junction proteins (ZO-1 and occludin), which were reduced after LPS injection (Fig. 5e and f); the results indicated that the reparative effects of dietary L-Trp are weaker than the protective effects on tight junction proteins.
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| Fig. 5 The effect of dietary L-Trp on the expression of (a) Claudin-1, (b) occludin, and (c) ZO-1 in LPS-induced Caco-2 cells. Fully differentiated Caco-2 cells are incubated with 20, 40 and 80 μM dietary L-Trp (24 h) after (a–c, left) or at the same time (d–f, right) as 100 μg mL−1 LPS (24 h). Cells without tryptophan and LPS treatments are designated as the negative control (NC) and those treated with LPS only as the positive control (PC). Results are expressed as protein expression (normalized with β-actin) relative to unstimulated cells as mean ± SD of three independent experiments. Different letters indicate significant differences using Tukey's multiple comparison test, α = 0.05. | ||
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