Issue 49, 2023

Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

Abstract

A split G-quadruplex (G4)-programmed Cas12a platform was established, validated, and optimized. The split G4 motif was recruited as substrate for Cas12a, and the label-free sensing platform provided a concentration-dependent response towards the input target. Furthermore, exosomal surface proteins from cultured cancer cells and clinical samples were detected and profiled.

Graphical abstract: Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

Supplementary files

Article information

Article type
Communication
Submitted
13 Mar 2023
Accepted
17 May 2023
First published
31 May 2023

Chem. Commun., 2023,59, 7615-7618

Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

M. Xu, Y. Zhu, Y. Zhang, B. Gong, Y. Kang, G. Su and Y. Yu, Chem. Commun., 2023, 59, 7615 DOI: 10.1039/D3CC01239E

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