Simple and fast microderivatization method for determining formaldehyde using narrow-bore liquid chromatography with UV detection

Abstract

Formaldehyde is a harmful substance that can cause sick building syndrome and other diseases, such as contact allergy, asthma, leukemia, cancer, and brain/neuron disorders. Formaldehyde is a ubiquitous chemical owing to its use in many common products, including as a preservative in household and personal care products. To prevent overexposure to formaldehyde, a simple method for determining and controlling the formaldehyde content in commercial products is required. In this study, 3-aminoquinoline (3-AQ) was used to derivatize formaldehyde under mild conditions (2 min at 30 °C) without the use of catalysts or activators. The derivatized sample solutions were separated using narrow-bore liquid chromatography with an ultraviolet (UV) detector in a run time of only 5 min. All sample extraction and derivatization protocols were performed on the microliter scale to reduce the use of organic solvents. The linear range for the determination was 5–1000 μg mL⁻¹, with a detection limit of approximately 1 μg mL⁻¹ (2 ng per 2 μL injection). The proposed microscale method was successfully applied to the analysis of formaldehyde in commercial household products.