Investigation into self-assembled collagen arrays guided by the surface properties of polyimide films

Abstract

The mechanism of highly-oriented collagen (Col) fibril arrays on rubbed polyimide (PI) films was investigated in order to understand the interfacial Col–PI interactions. It was found that the orientation of the surface functional groups of the rubbed PI films was most effectively controlled and optimized by the rubbing conditions. In particular, nano-grooves with a width of 100–600 nm and a depth of 2–10 nm were formed on the rubbed PI films at a rubbing strength of 2.4 m, leading to the formation of the highest density of the Col fibril array. Moreover, highly-oriented Col fibrils were formed inside the nano-grooves by the formation of hydrogen bonds between the CO of the imide groups (@ rubbed PI films) and the N–H of the amino groups (@ β-Sheets of Col molecules), resulting in the orientation of the Col molecules and subsequent assembly to the fibrils. Thus, the orientation and density of the fibril arrays on the rubbed PI films were successfully controlled by the interfacial interactions between the β-Sheet component of Col and the nano-groove surfaces of the rubbed PI films. Therefore, the novel technology of this study will provide an effective method to fabricate the one-directional fibrous nanostructures and to understand how to control the orientation of biomolecules in vitro.