Dong-Sheng
Ji
a,
Hui
Liang
a,
Kai-Xuan
Yang
a,
Zhi-Tao
Feng
c,
Yong-Chun
Luo
*a,
Guo-Qiang
Xu
a,
Yucheng
Gu
d and
Peng-Fei
Xu
*ab
aState Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, P. R. China. E-mail: xupf@lzu.edu.cn; luoych@lzu.edu.cn
bState Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, 730000, P. R. China
cDepartment of Chemistry, University of California Davis, One Shields Avenue, Davis, California 95616, USA
dSyngenta Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, UK
First published on 18th January 2022
A protocol for the chemically divergent synthesis of β-lactams and α-amino acid derivatives with isothiourea (ITU) catalysis by switching solvents was developed. The stereospecific Mannich reaction occurring between imine and C(1)-ammonium enolate generated zwitterionic intermediates, which underwent intramolecular lactamization and afforded β-lactam derivatives when DCM and CH3CN were used as solvents. However, when EtOH was used as the solvent, the intermediates underwent an intermolecular esterification reaction, and α-amino acid derivatives were produced. Detailed mechanistic experiments were conducted to prove that these two kinds of products came from the same intermediates. Furthermore, chemically diversified transformations of β-lactam and α-amino acid derivatives were achieved.
β-lactams and α-amino acid derivatives play important roles in life events.5 β-lactams refer to a large class of antibiotics with a β-lactam ring in their chemical structure, including the most commonly used penicillins and cephalosporins in clinical practice, as well as newly developed cephamycins and thiobacillus antibiotics, monocyclic β-lactams and other atypical β-lactam antibiotics, such as aztreonam and zetia.6 Amino acids are the basic units of proteins, which are mainly used as therapeutic drugs and as basic units of synthetic peptide drugs (Fig. 1).7 Therefore, it is essential to develop concise and efficient synthetic methods for the synthesis of chiral β-lactams and α-amino acid derivatives.8 Over the past few decades, the synthesis of β-lactams mediated by chiral Lewis base nucleophilic catalysts has progressed very well. Lectka developed cinchona alkaloid participating reactions for accessing chiral β-lactams.9 Fu reported planar-chiral DMAP involving protocols for obtaining chiral β-lactams.10 Birman and Smith have carried out pioneering studies in this field via C1 ammonium generated from chiral isothiourea11 (Scheme 2A). Although there are some reports on the construction of chiral β-lactams or amino acids, all these reactions were developed for the synthesis of only one kind of them.8c,11c In terms of chemistry, these two kinds of compounds are structurally closely linked to each other, and we envisioned that they could be synthesized from the same intermediates as in biosynthesis. Therefore, we attempted to synthesize these two important kinds of molecules exploiting the strategy of divergent synthesis, which will contribute to enriching potential candidates for screening and discovering drugs.
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Scheme 2 (A) Chiral Lewis base nucleophilic catalysts used for the synthesis β-lactams. (B) Pioneering advances about isothiourea organocatalysis. (C) The proposed protocol. |
In recent years, isothiourea (ITU) catalysis, as a new kind of organocatalysis, has attracted tremendous attention from organic chemists,12 and not only shows outstanding performance in controlling product stereoselectivity but can also be combined with transition metal,13 photo14 and amine catalysis.15 Smith, as a pioneer in this field, developed a new homobenzotetramisole (HBTM) catalyst and achieved many [2 + 2] and [4 + 2] cycloaddition processes, with which many useful molecules have been constructed.16 Snaddon developed a method for the enantioselective α-functionalization of acyclic esters through a synergistic combination of ITU catalysis and transition metal catalysis.17 In 2017, Gong reported an asymmetric [4 + 2] annulation of C1 ammonium enolates generated from chiral isothiourea catalysts and copper–allenylidene complexes. Later, a new strategy combining the palladium-catalyzed carbonylation and chiral ITU catalysis was developed to access dihydropyridones and β-lactams by the same group (Scheme 2B).18 There are also other chemists who have devoted their efforts to this field and promoted the development of ITU catalysis.19 Based on the principle of divergent synthesis, ITU catalysis, the longstanding exploration in organocatalysis20 and the hypothesis that β-lactams and α-amino acid derivatives may be produced from the same intermediates, a strategy for the divergent synthesis of β-lactams and α-amino acid derivatives by switching reaction solvents was proposed. We envisioned that a stereospecific Mannich reaction between C(1)-ammonium enolates and imines would produce key intermediate I (Scheme 2C). Then, there would be two different pathways to regenerate the catalyst from intermediate I: one is the process of the intramolecular lactamization, and the other is the process of the intermolecular esterification when ethanol is used as the solvent. Therefore, β-lactams and α-amino acid derivatives would be easily obtained from using different solvents. There are two obvious challenges in the protocol: one is the competitive intramolecular and intermolecular reactions of intermediates I, which will determine whether or not we can access either only β-lactams or only α-amino acid derivative products; the other is the diastereoselectivity issue because imines might have two pathways to approach enolates.
Entrya | Cat. | Solvent | Yield 3cab | Yield 4cab | drc | eed |
---|---|---|---|---|---|---|
a Reactions performed with 0.1 mmol 1a, 0.1 mmol 2a, 0.1 mmol i-Pr2NEt, catalyst (20 mol%), in solvent (1 mL) at 15 °C for 24 hours.
b Isolated yield of the major isomer.
c Determined by 1H NMR analysis of the crude products.
d Determined by chiral-phase HPLC.
e The reaction was performed at 0 °C for 40 hours.
f The reaction was performed at −40 °C.
g The reaction was performed at −50 °C for 48 hours, CH3CN/DCM = 3![]() ![]() |
||||||
1 | Cat.1 | CH2Cl2 | 53 | — | 3![]() ![]() |
90 |
2 | Cat.2 | CH2Cl2 | 56 | — | 3![]() ![]() |
85 |
3 | Cat.3 | CH2Cl2 | 51 | — | 3![]() ![]() |
78 |
4 | Cat.4 | CH2Cl2 | 42 | — | 2![]() ![]() |
74 |
5 | Cat.5 | CH2Cl2 | 38 | — | 3![]() ![]() |
91 |
6 | Cat.1 | CHCl3 | 56 | — | 3![]() ![]() |
88 |
7 | Cat.1 | Toluene | Trace | — | — | — |
8 | Cat.1 | CH3CN | 70 | — | 3![]() ![]() |
90 |
9 | Cat.1 | EtOH | — | 72 | 5![]() ![]() |
92 |
10e | Cat.1 | CH3CN | 72 | — | 3![]() ![]() |
91 |
11e | Cat.1 | EtOH | — | 78 | 20![]() ![]() |
99 |
12f | Cat.1 | CH3CN | 80 | — | 8![]() ![]() |
99 |
13g | Cat.1 | CH3CN | 78 | — | 8![]() ![]() |
98 |
14g,h | Cat.1 | CH3CN | 85 | — | 8![]() ![]() |
99 |
15e,h | Cat.1 | EtOH | — | 81 | 20![]() ![]() |
99 |
With the optimal reaction conditions established, the substrate scope to synthesize β-lactams was explored (Table 2). First, the electronic and steric effects on aryl acetic acid esters were explored. Acetic acid esters containing para- and meta-substituted phenyls all gave desirable products with excellent enantioselectivities and diastereoselectivities (3cb–3cg), while substrates containing electron withdrawing groups (EWG) needed more reaction time than substrates containing electron donating groups (3cb, 3cg). By comparison, an obvious steric effect was observed when ortho-substituted phenylacetic acid ester was used, which produced 3ch and 3ci as a 5:
1 mixture of diastereomers with good yields and excellent enantioselectivity (99% ee). Ortho-methyl substituted substrate 2j with less steric hindrance gave ideal results with higher enantioselectivity (99% ee) and diastereoselectivity than ortho-chlorine- or bromine-substituted substrates. Next, both naphthyl and thienyl acetic acid esters gave desirable products (3ck, 3cl) with satisfying results. Substrates 2 possessing multiple substituents were also compatible with the protocol, which produced the corresponding products (3cm, 3cn) with excellent stereoselectivities and yields. To demonstrate the value of this protocol for the synthesis of β-lactams, commercial pharmaceutical indomethacin-derived β-lactam 3cp was constructed with moderate stereoselectivities and yield. In general, imines 1 containing either EWG- or EDG-substituted Ar1 afforded products with satisfactory results. When the phenyl group of imines was changed to a thienyl group, the desired products were obtained with excellent stereoselectivities in modest yields. When the ester group of imines 1 was turned into either methyl ester or benzyl acetate, the expected products were obtained with similar stereoselectivities and yields. The absolute configuration of product 3cj (CCDC 2077824) was determined to be (2S,3S) by single-crystal X-ray diffraction analysis, and the structures of other products were assigned by analogy.23
a All reactions were performed with 20 mol% of Cat.1, 0.1 mmol of 1, 0.12 mmol of 2, and 0.1 mmol i-Pr2EtN in 0.75 mL CH3CN and 0.25 mL CH2Cl2 at −50 °C for t h; isolated yields of major isomers are provided. |
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Next, the universality of EtOH-directed synthesis of α-amino acid derivatives was studied (Table 3). Generally, this reaction showed better diastereoselectivity than the synthesis of β-lactams, and all substrates afforded products with excellent stereoselectivities. Substrates 2 with EWG produced the expected products with lower yields than substrates with EDG (4cb, 4cd to 4cc, 4ce). Substrates containing meta-position-substituted Ar2 all afforded desirable products in excellent results without apparent substituent effects. Vicinal larger steric substituents resulted in lower yields and stereoselectivities (4ci and 4cj). Satisfying results were observed for substrates containing Ar2 with multiple substituents (4ck and 4cl). The substrate with an aryl group replaced by a 3-thienyl group yielded the corresponding product with an acceptable outcome (4cm). Furthermore, the commercial antiphlogistic drug indomethacin was also compatible with this protocol, and the corresponding amino acid derivative 4cn was obtained with a modest yield and stereoselectivities. The generality of substrates of imines 1 was also inspected. As shown in Table 3, the reaction results showed excellent stereoselectivities (mostly 99% ee), and the expected products were obtained along with modest to high yields when there was an EDG group on Ar.1 A fluorine-substituted phenylethyne-derived imine furnished the product with a diminished yield and excellent enantioselectivity. The heteroaryl substrate was also compatible with this protocol and afforded the desirable products with moderate stereoselectivities and yields, such as 4ka. The steric hindrance of the ester group in imines 1 was also investigated; when the COOEt group was changed to a COOMe or COOBn group, the corresponding α-amino acid derivatives were still obtained with excellent results. The absolute configuration of product 4cm (CCDC 2111690) was determined to be (2S,3S) by single-crystal X-ray diffraction analysis, and the structures of other products were assigned by analogy.24
a All reactions were performed with 20 mol% of Cat.1, 0.1 mmol of 1, 0.12 mmol of 2, and 0.1 mmol i-Pr2EtN in 1 mL of EtOH at 0 °C for t h; isolated yields are provided. |
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Having explored the reaction substrate scopes of this protocol, we turned our attention to the mechanism of this divergent synthesis strategy. First, under the optimal conditions to synthesize α-amino acid derivatives, the ethanolysis experiment of optically pure β-lactam 3ca was conducted (Scheme 3A and B). The ring opening product was not detected, and a diastereoisomer of 3ca was observed. These results elucidated that the α-amino acid derivatives do not come from the ethanolysis of β-lactams under the reaction conditions and supported the assumption that the epimerization of β-lactams contributed to lower diastereoselectivities under basic conditions,25 which was mentioned earlier in the article. A mixture solvent experiment was carried out under the optimal conditions of synthesizing β-lactams, and the only product was an α-amino acid derivative (Scheme 3B). We proposed that there would be two competitive routes in the process of regenerating the ITU catalyst. The oxygen atom in EtOH as a nucleophile prevails compared with the nitrogen atom in the substrate; therefore, the intramolecular esterification reaction occurs at a higher level than the intermolecular lactamization reaction. With this protocol, the chemical divergent synthesis of β-lactams and α-amino acid derivatives was truly directed by solvents.
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Scheme 3 (A) The isolated product as starting material in ethanolysis. (B) The isolated product as an additive in reaction of another substrate. (C) Mixed solvent experiment. |
Based on these experimental results (nonlinear effect and deuterium experiments, see the ESI†) and other literature reports,12,14,16 a proposed mechanism is outlined in Fig. 2. Initially, an N-acylation occurs between BTM and the ester, which gives acyl ammonium ion pair I. Ammonium enolate II is generated from the process of deprotonation,16d,26 which is facilitated by i-Pr2NEt as an auxiliary base. Then, a stereoselective Mannich reaction of ammonium enolate II with imine occurs to form the key zwitterionic intermediate III. Subsequently, when acetonitrile is used as the solvent, lactamization will give β-lactam products and regenerate the catalyst BTM; otherwise, esterification gives α-amino acid derivatives and regenerates the catalyst BTM when ethanol is used as the solvent. The stereochemical outcome of the reaction is determined in the step of the Mannich reaction, where ammonium enolate II adopts a Z-conformation aided by the 1,5-O S interaction (chalcogen-bonding catalysis)27 between the enolate oxygen anion and the S atom of the catalyst, which provides a conformational lock. The phenyl group shields the Si face, and the imine approaches from the least hindered Re face and adopts the favored transition state28 with the help of π–π stacking interaction that comes from the phenyl group of the enolates and alkynyl group of the imines, which determines the configuration at C3 of the products. The stereochemical results are consistent with single X-ray crystal experiments.
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Fig. 2 Proposed mechanism for the solvent directed divergent synthesis of β-lactam and α-amino acid derivatives. |
To prove the practicability of the protocol, some gram-scale reactions were conducted. Target products 3ca and 4ca were accessed with higher yields and similar stereoselectivities compared with the model reaction when the reaction scale was amplified 40-fold (Scheme 4A). In view of the potential value of alkynyl groups in synthetic chemistry, further transformation of the group was tested (Scheme 4B). It is worth emphasizing that a substituent-directed transformation of alkynyl groups in 3ca and 4ca was achieved. Under the conditions of using Ag/Au salts29 as catalysts in toluene, a process of intermolecular hydroxylation/isomerization mediated by Au occurred for 4ca and gave product 5 with 71% yield and 99% ee. Interestingly, an intramolecular process occurred for 3ca to give product 6 with a moderate yield but without a loss of enantioselectivity. Moreover, divergent hydrogenation of 4ca was achieved by using Pd/C or Pd/CaCO3 as the catalyst, which produced product 7 or 8, respectively, with good results.
Footnote |
† Electronic supplementary information (ESI) available. CCDC 2077824, 2111690, 2118205. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/d1sc06127e |
This journal is © The Royal Society of Chemistry 2022 |