Issue 45, 2022

Ring -opening of polythiolactones to construct protein-imprinted nanospheres with high recognition and regulation capabilities

Abstract

Developing and preparing novel protein-imprinted nanomaterials with high recognition ability remains challenging because it is difficult to controllably and orderly design and arrange functional groups on the imprinted polymer layers of protein-imprinted nanomaterials to improve their protein identification. Herein, we present a new technology using rationally designed polythiolactone-decorated magnetic nanospheres as the precursor of multifunctionalized imprinted materials. Moreover, the strategy of ring-opening the polythiolactione layers using primary amines with terminal alcohols, acids and pyrrolidines introduces abundant recognition sites, which enhance the recognition for template proteins through multiple hydrogen-bonding and hydrophobic interactions. Thiols generated in situ by the ring-opening reaction provide sufficient crosslinking sites proximate to each recognition site for the formation of imprinting cavities, endowing the imprinted nanospheres with promising regulation capabilities. Based on the rational design, the imprinted nanospheres can be prepared conveniently and present tunable rebinding capacity and specificity for bovine serum albumin (BSA). The maximum saturated rebinding capacity of imprinted materials for BSA is up to 285 ± 15 mg g−1 and the highest imprinting factor reaches 5.79. The simple and versatile strategy demonstrated in this study shows promise for the design of other protein-imprinted materials with high recognition ability.

Graphical abstract: Ring -opening of polythiolactones to construct protein-imprinted nanospheres with high recognition and regulation capabilities

Supplementary files

Article information

Article type
Paper
Submitted
07 Jul 2022
Accepted
07 Oct 2022
First published
12 Oct 2022

Nanoscale, 2022,14, 16865-16873

Ring -opening of polythiolactones to construct protein-imprinted nanospheres with high recognition and regulation capabilities

M. Wang, G. Zhang, Q. Liu, M. Wei, Y. Ren, S. Fa and Q. Zhang, Nanoscale, 2022, 14, 16865 DOI: 10.1039/D2NR03715G

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