Rosaria
Villano
*a,
Hannah
Straker
b and
Vincenzo
Di Marzo
ac
aIstituto di Chimica Biomolecolare, CNR, Via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy. E-mail: rosaria.villano@icb.cnr.it
bGW Pharma part of Jazz Pharmaceuticals, Kent Science Park, Sittingbourne, Kent ME9 8AG, UK
cCanada Excellence Research Chair on the Microbiome-Endocannabinoidome Axis in Metabolic Health, Faculty of Medicine and Faculty of Agricultural and Food Sciences, Centre de Recherche de l’Institut de Cardiologie et Pneumologie de l’Université et Institut sur la Nutrition et les Aliments Fonctionnels, Centre NUTRISS, Université Laval, QC G1V 4G5, Quebec City, Canada
First published on 14th October 2022
Alkylresorcinols (ARs) are phenolic lipids present in several plants and, from a synthetic point of view, are also versatile key intermediates for the production of many biologically active molecules. In this work, we describe a general and efficient method for the preparation of ARs, also in deuterated form. Finally, this methodology was conveniently exploited for a very rapid approach to synthesize cannabidiol (CBD) and cannabidivarin (CBDV), two non-psychotropic cannabinoids with a bicyclic scaffold present in Cannabis sativa.
ARs, in addition to being target products, are also very common structural subunits in natural and pharmaceutically active products. As such, they are extensively used in organic chemistry as versatile starting materials for the synthesis of a broad range of complex molecular scaffolds: coumarins,4 2,8-dioxabicyclo[3.3.1]nonanes,5 isoflavones,6 benzopyrans7 and cannabinoids8 (Scheme 1).
In the light of these considerations, the development of effective and versatile protocols for their synthesis is highly desirable. In this context, the design of practical synthetic strategies for the production of several structurally different ARs using a minimum number of steps and readily available inexpensive starting materials, as well as the elaboration of methodologies that can also be exploited for the synthesis of deuterated analogues, is a fascinating challenge.
Generally, traditional approaches for the synthesis of ARs involve Grignard and related reactions, palladium-catalysed Suzuki coupling, reductive alkylation followed by oxidative decarboxylation or Wittig reaction (Scheme 2).1,9–13 Some of these strategies are characterized by a rather high number of steps that inevitably lead to a reduction in the total yield of the process, expensive reagents, highly reactive organometallic species which require an accurate control of experimental parameters and very dry conditions. A further disadvantage is that only some of such strategies have also been exploited for the synthesis of the corresponding deuterated analogues (above all with short saturated alkyl chain).14–16
We report here a simple methodology for the preparation of ARs, as such or chemoselectively deuterated, in high yields.
Finally, in order to explore further the synthetic utility of this strategy, ARs produced through this optimized procedure were used as starting materials to synthesize two non-psychotropic bicyclic cannabinoids17,18 (CBD and CBDV) and their deuterium labelled analogues via conventional terpenylation. The elaboration of new efficient synthetic procedures for the production of cannabinoids, in order to produce adequate quantities of products for clinical uses, is strongly desirable for their promising therapeutic potential (migraine and headaches, glaucoma, convulsions, etc.).19–22 In particular, the development of protocols potentially applicable to automated processes (flow synthesis) represents a future challenge. In this regard, the first automation of the terpenylation step23 of the synthesis of CBD and CBDV for possible large-scale industrial applications opens up new perspectives and renders the development of simple and efficient methodologies for the production of ARs even more important. In addition, having versatile methodologies that can also be used for the synthesis of deuterated ARs and, consequently, deuterated cannabinoids suitable as internal standards for mass spectrometric analyses, or as starting materials for the production of deuterated metabolites for the study of cannabinoid metabolism and pharmacokinetics in animals and humans, is also important.
The following Pd/C-catalysed reduction of the triple bond in 4 with H2 in H2O/THF or alternatively in EtOH provided the product 5 with the suitable saturated side chain (C3 in 5a and 5c or C5 in 5b) and its subsequent demethylation16 by BBr3 in CH2Cl2 gave the ARs 1a–c.
Therefore, deuterated ARs could be prepared with the same synthetic strategy in Scheme 3 by using deuterated alkyl iodides 3, but also in a very convenient and economic way. The triple bond of the product 4 was not only able to be easily and selectively hydrogenated but also deuterated thus allowing the introduction of four deuterium atoms in the alkyl chain. This approach is particularly useful and convenient when the appropriate deuterated alkyl iodide is not commercially available or is too expensive.
In fact, when the Pd/C-catalysed reduction of the triple bond with H2 was realized in D2O/THF instead of H2O/THF,26,27 the complete deuteration of the triple bond afforded the deuterated products 5d and 5e in quantitative yields (Scheme 4). Therefore, the Pd/C-catalysed H2–D2 exchange reaction using H2–D2O provided an efficient, cheap and environmentally friendly way for the in situ preparation of D2. Gratifyingly, these reactions were efficient, with >95% deuterium incorporation,28 no competitive deuteration of the aromatic ring observed, and a site-selective incorporation of deuterium on the alkyl chain achieved.
Scheme 4 Synthetic sequence for the production of deuterated ARs via deuteration of the triple bond. |
All reactions of this multistep synthetic sequence (Schemes 3 and 4) were cleaned and characterized by very simple workups; ARs 1a–e (deuterated or not) were isolated in high yields (≥ 87% overall yield from 2, higher than those reported in the literature using different synthetic strategies23) over only 3 steps without isolation of the intermediate products 4 and 5, thus allowing a beneficial reduction in the amount of used organic solvents.
Importantly, the methodology is very versatile and it could be potentially used to prepare new ARs with different length or completely deuterated side chains, simply by appropriately selecting the alkyl halide 3 for the alkylation reaction (first step) and the solvent for the triple bond reduction (second step). Finally, this protocol was readily scalable (up to 10 mmol) without substantial change in efficiency.
To further explore the synthetic value of this strategy, we applied the methodology described here to prepare two bicyclic cannabinoids (CBD and CBDV) and their deuterated analogues.
As depicted in Scheme 5, all ARs 1a–e were condensed with commercially available (1S,4R)-4-isopropenyl-1-methyl-2-cyclohexen-1-ol 6 in the presence of 33 mol% wet pTSA to afford the trans bicyclic cannabinoids as a mixture of normal and abnormal scaffolds.8 The products 7 (up to 26% yield) and 8 (up to 38% yield) were isolated after a chromatographic column and, importantly, under these experimental conditions, no tricyclic structure was produced. The yields of this last reaction of terpenylation were comparable with those reported in the literature.8
The coupling of ARs 1b and 1e with 6 afforded CBD 7b and its deuterated analogue 7e, respectively, as confirmed by the 1H-NMR spectra. The 1H NMR spectra of botanical purified CBD supplied by GW Research Ltd, now a part of Jazz Pharmaceuticals, Inc., and synthetic CBD 7b matched perfectly (Fig. 1, blue vs. red) confirming that the structure and the relative configuration of the stereogenic centers were the same (trans). The absolute configuration of natural CBD was elucidated by Mechoulam as R at both C-3 and C-4.29 Also, in the synthetic CBD 7b the absolute configuration at C-4 was R because it was derived from the chiral building block 6 (chiral pool approach). Consequently, the configuration at C-3 was also assigned as R, as in botanical CBD. In parallel, the spectrum of 7e, while confirming all the previous considerations, shows loss of signals at 2.4–2.5 ppm and 1.6 ppm due to the deuterium contributions (Fig. 1, green).
Fig. 1 1H NMR spectra of pure botanical CBD supplied by GW Research Ltd, now a part of Jazz Pharmaceuticals, Inc. (blue), synthetic CBD 7b (red) and its deuterated analogue 7e (green). |
A similar NMR comparison was also realized for pure botanical CBDV supplied by GW Research Ltd, now a part of Jazz Pharmaceuticals, Inc., (Fig. 2, blue), synthetic CBDV 7a (Fig. 2, red) and its deuterated analogues 7c and 7d (Fig. 2, green and purple respectively) with similar results to those highlighted for CBD.
Key advantages of this synthetic sequence are that all reactions led to the corresponding products with high yields, without the formation of side-products and requiring only column chromatographic purification of the final AR, with a reduction in the amount of solvent and energy needed for separation and purification.
All these features make this strategy particularly useful for the production of ARs on a medium scale, allowing them to be used as starting materials in the synthesis of complex molecular scaffolds (as exemplified here by the application of the method to two bicyclic cannabinoids and their deuterium labelled analogues), under batch but also, in perspective, in flow chemistry conditions for possible large-scale industrial applications.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2nj03547b |
This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2022 |