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DOI: 10.1039/D2CC90389J (Correction) Chem. Commun., 2022, 58, 12740-12740

Correction: Use of pyridazinediones as extracellular cleavable linkers through reversible cysteine conjugation

Calise Bahou a, Richard J. Spears a, Abil E. Aliev a, Antoine Maruani a, Marcos Fernandez a, Faiza Javaid a, Peter A. Szijj a, James R. Baker *a and Vijay Chudasama *ab
aDepartment of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK. E-mail: v.chudasama@ucl.ac.uk; j.r.baker@ucl.ac.uk
bResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-004 Lisbon, Portugal

Received 24th October 2022 , Accepted 24th October 2022

First published on 4th November 2022

Abstract

Correction for ‘Use of pyridazinediones as extracellular cleavable linkers through reversible cysteine conjugation’ by Calise Bahou et al., Chem. Commun., 2019, 55, 14829–14832, https://doi.org/10.1039/C9CC08362F.

The authors regret that there was an error in the labelling of the associated mass spec insert in Fig. 3(b)(i) in the original manuscript. The tallest peak was incorrectly labelled 29[thin space (1/6-em)]357.0 Da (erroneously referring to GFP 14). This peak should instead be labelled 29[thin space (1/6-em)]537.0 Da (referring to GFP–PD 16). The shorter peak to the left of this peak should be labelled 29[thin space (1/6-em)]365.5 Da (referring to GFP 14). “Calculated 29[thin space (1/6-em)]355.0 Da, Found 29[thin space (1/6-em)]357.0 Da” should read “Calculated 29[thin space (1/6-em)]355.0 Da, Found 29[thin space (1/6-em)]365.5 Da”. This does not affect the results or conclusions of the paper.

The corrected version of Fig. 3 is presented here.


image file: d2cc90389j-f3.tif
Fig. 1 (a) Mass spectrometry data showing (i) GFPS147C 14 and model Fab fragment 15; (ii) GFP–PD species 16 and Fab–PD species 17; (iii) GFP-maleimide species 18 and Fab-maleimide species 19; (b) mass spectrometry analysis of (i) incubation of GFP conjugates 16 and 18 (50 μM) in PBS pH 7.4 for 7 days at 37 °C; (ii) incubation of Fab conjugates 17 and 19 (20 μM) in PBS pH 7.4 for 7 days at 37 °C. (c) Kinetic analysis of reaction between GFP conjugates (16 and 18) and DTNB 12 in PBS pH 7.4 for 12 h at 37 °C to form TNB 13 that was monitored by UV absorbance at A412. Plots show changing [TNB] and ln[GFP conjugate] vs. time.

The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.

This journal is © The Royal Society of Chemistry 2022
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