Changjia Hu, Junbo Chen, Peng Yang, Lijie Du, Lingying Xia, Juan He and Xiandeng Hou
Chem. Commun., 2022,58, 10643-10646
DOI:
10.1039/D2CC03995H,
Communication
Herein, we developed a nucleic acid amplification process monitoring scheme by the use of UiO-66-NH2, in which pyrophosphate ion (PPi) released from the amplification can competitively coordinate with Zr to weaken the ligand-to-metal charge transfer and cause fluorescence recovery, thus reflecting the amplification process. This is the first attempt at using a MOF for monitoring the nucleic acid amplification process.
