Correction: Interrogating cardiac muscle cell mechanobiology on stiffness gradient hydrogels

Abstract

Correction for ‘Interrogating cardiac muscle cell mechanobiology on stiffness gradient hydrogels’ by Ian L. Chin et al., Biomater. Sci., 2021, 9, 6795–6806, https://doi.org/10.1039/D1BM01061A.

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The authors regret that an incorrect panel was inserted into Fig. 5 in the original manuscript. The correct Fig. 5 is as shown here.

Fig. 5 Morphology and mechanomarker expression in neonatal rat cardiomyoctes (NRCMs). Representative immunofluorescent images of NRCMs cultured on Col-coated substrates, stained for DAPI, F-actin and (A) Lamin-A or (B) YAP. (C) Analysis DAPI stain did not reveal any significant relationships between substrate stiffness and nuclei size (Pearson's correlation Col – R² = 0.01, P > 0.05, n = 5; Fn R² = 0.10, p > 0.05, n = 7; Ln R² = 0.20, P > 0.05, n = 5), (D) substrate stiffness and nuclei form factor (Pearson's correlation Col – R² = 0.06, P > 0.05, n = 5; Fn R² = 0.11, p > 0.05, n = 7; Ln R² = 0.01, P > 0.05, n = 5) and (E) substrate stiffness and nuclei aspect ratio (Pearson's correlation Col – R² = 0.02, P > 0.05, n = 5; Fn R² = 0.01, p > 0.05, n = 7; Ln R² = 0.01, P > 0.05, n = 5). (F) A positive correlation was observed between Lamin-A expression and stiffness for cells cultured on Fn coated hydrogels, but not on Col or Ln coated hydrogels (Pearson's correlation, Col – R² = 0.11, P > 0.05, n = 4; Fn – R² = 0.85, P < 0.05, n = 5; Ln – R² = 0.21, P > 0.05, n = 5). (G) Similarly, YAP was increasingly nuclear localised with increasing stiffness on cells cultured on Fn-coated hydrogels but not Col- or Ln-coated hydrogels (Pearson's correlation, Col – R² = 0.37, P > 0.05, n = 4; Fn – R² = 0.79, P < 0.05, n = 5; Ln – R² = 0.004, P > 0.05, n = 5). All graphs show mean ± SEM.

The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.

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