Issue 12, 2021

The effects of nanobubbles on the assembly of glucagon amyloid fibrils

Abstract

Some recent studies have shown that the surface and interface play an important role in the assembly and aggregation of amyloid proteins. However, it is unclear how the gas–liquid interface affects the protein assembly at the nanometer scale although the presence of gas–liquid interfaces is very common in in vitro experiments. Nanobubbles have a large specific surface area, which provides a stage for interactions with various proteins and peptides on the nanometer scale. In this work, nanobubbles produced in solution were employed for studying the effects of the gas–liquid interface on the assembly of glucagon proteins. Atomic force microscopy (AFM) studies showed that nanobubble-treated glucagon solution formed fibrils with an apparent height of 4.02 ± 0.71 nm, in contrast to the fibrils formed with a height of 2.14 ± 0.53 nm in the control. Transmission electron microscopy (TEM) results also showed that nanobubbles promoted the assembly of glucagon to form more fibrils. Thioflavin T (ThT) fluorescence and Fourier transform infrared (FTIR) analyses indicated that the nanobubbles induced the change of the glucagon conformation to a β-sheet structure. A mechanism that explains how nanobubbles affect the assembly of glucagon amyloid fibrils was proposed based on the above-mentioned experimental results. Given the fact that there are a considerable amount of nanobubbles existing in protein solutions, our results indicate that nanobubbles should be considered for fully understanding the protein aggregation events in vitro.

Graphical abstract: The effects of nanobubbles on the assembly of glucagon amyloid fibrils

Article information

Article type
Paper
Submitted
30 Dec 2020
Accepted
07 Feb 2021
First published
08 Feb 2021

Soft Matter, 2021,17, 3486-3493

The effects of nanobubbles on the assembly of glucagon amyloid fibrils

Y. Wang, Z. Guo, T. Tan, Y. Ji, J. Hu and Y. Zhang, Soft Matter, 2021, 17, 3486 DOI: 10.1039/D0SM02279A

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