Hong-Bo Liu, Hai Xu, Xin Guo, Jian Xiao, Zheng-Hong Cai, Ya-Wen Wang and Yu Peng
Org. Biomol. Chem., 2021,19, 873-877
DOI:
10.1039/D0OB02405H,
Paper
A dicyanoisophorone/acrylate-combined probe (DDP) was synthesized and designed as a near-infrared (NIR) fluorescent sensor for the rapid identification of Cys over Hcy and GSH in aqueous solution with a large Stokes shift (143 nm). The detection limit of Cys was 1.23 μM, which was lower than that of the intracellular Cys concentration. DDP was cell membrane-permeable and had been successfully applied to the detection of intracellular Cys in HeLa cells. The detection mechanism was determined by 1H NMR titration, MS and DFT calculations.