Siavash
Iravani
*a and
Rajender S.
Varma
b
aFaculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. E-mail: siavashira@gmail.com
bRegional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 783 71 Olomouc, Czech Republic
First published on 7th April 2021
In view of their unique planar structure and outstanding physical and chemical properties, two-dimensional (2D) materials have garnered the attention of interdisciplinary researchers in the domain of biomedical and clinical applications. MXenes are 2D transition metal carbides and nitrides with outstanding characteristics, comprising huge surface area, biocompatibility, low toxicity, significant electrical conductivity, antibacterial activity and hydrophilicity. Although numerous investigations have demonstrated the promising potential of MXenes for different biomedical applications that include biosensing, bioimaging, cancer therapy, tissue engineering, regenerative medicine, and drug delivery, there are still important challenging issues pertaining to their stability in physiological environments, sustained/controlled release of drugs, and biodegradability. The well-designed ultra-thin MXene nanosheets are deployable as promising biocompatible inorganic nanoplatforms for assorted biomedical applications via the clinical translation of nanomedicine; MXenes are good candidates for tissue engineering and regenerative medicine. Herein, recent progresses on 2D MXenes for state-of-the-art tissue engineering and regenerative medicine are discussed with emphasis on the significant challenges and future perspectives.
MXenes are two-dimensional (2D) transition metal carbides and nitrides, where Ti3C2Tx exemplifies the first candidate prepared by etching an Al layer in Ti3AlC2, Tx being the surface groups (e.g., –F and –OH) generated during the etching process (Table 1).7,8 MXenes and MXene-based materials with remarkable electronic conductivity, elastic mechanical strength, hydrophilicity, and chemical stability have promising potentials for deployment in the field of environmental science/water treatment, energy storage, composite materials, drug/gene delivery, and biomedical/pharmaceutical sciences,9–14 and likewise have been utilized for biosensing, antibacterial materials, bioimaging, theranostic nanomedicine, and cancer diagnosis/therapy.8,15–19 They have been employed in biosensing and bioimaging comprising magnetic resonance and photoacoustic imaging techniques in view of their unique tunable optical and magnetic properties. Similar interesting potentials have been uncovered as efficient agents for environmental and antimicrobial treatments due to their pollutant adsorption and appropriate antibacterial properties.20–22
Properties | Remarks | Ref. |
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Mechanical properties | The mechanical properties of MXenes significantly depend on their surface terminations; MXenes with O termination demonstrated significant stiffness, but these materials with F or OH terminations exhibited low elastic stiffness. Notably, the surface-functionalized MXenes demonstrated larger flexibility; the improvement of mechanical features (e.g., toughness, compressive strengths, tensile, and flexibility) of MXenes can be performed via the production of MXene-based composites by applying various types of polymeric materials. | 14, 23 and 24 |
Electronic features | MXenes demonstrated different attractive electronic features, which included semiconductivity, topological insulativity, and metallicity; the conductivity of MXenes depends significantly on their fabrication and delamination techniques. | 25 and 26 |
Magnetic properties | Several 2D materials are nonmagnetic that can restrict their spintronics applications; however, some MXenes such as Ti2N, Cr2C, and Ti2C are ferromagnetic, and the others like Cr2N and Mn2C are antiferromagnetic. The magnetic features of MXenes can be simply adjusted by surface functionalization. | 27–30 |
Optical properties | MXenes exhibited strong plasmonic resonances, large optical transparency window, nonlinear optical performance, optical transparency, suitable photothermal conversion, optical response with tunability, and robust surface sensitive optical properties; the optical properties of MXenes can be tuned by adjusting the functional groups (e.g., functional nanoparticles) on their surfaces. | 12, 31 and 32 |
The conventional organic compounds with acceptable biocompatibility and biodegradability attributes were widely investigated for biomedical applications, but their poor thermal and chemical stability and solitary functionality are the main shortcomings restricting their development in clinical terms.33 On the other hand, 2D structures fabricated via bottom-up (e.g., template method, hydro/solvothermal synthesis, microwave-assisted synthesis, chemical vapor deposition, and topochemical transformation approach) and top-down (e.g., ion-change, ultrasonic, and micromechanical exfoliation) tactics have shown attractive properties for biomedical applications.34,35 As an example, selenium-coated tellurium nanoheterojunctions with nontoxicity advantages exhibited promising results for the eradication of hepatocellular carcinoma and lung cancer in preclinical models. In this study, the deployed photothermal therapy (PTT)-based approach could alter the microenvironment of tumors and stimulate significant apoptosis of cancerous cells.36 Additionally, black phosphorus nanosheets with large surface area, intrinsic photoacoustic features, biocompatibility, and biodegradability are potential candidates for diverse biomedical applications, including photothermal/photodynamic therapy, diagnostics imaging, biosensing, cancer diagnosis/therapy, drug/gene delivery, neuronal regeneration, and three dimensional (3D) printing scaffolds.37–39 For instance, to synergistically improve the efficacy of cancer therapy and management, a photothermal therapy tactic using black phosphorus was employed together with an anti-CD47 antibody-based immunotherapy strategy.40 It was indicated that the ablation of tumors via black phosphorus-based photothermal therapy stimulated the immunological responses specifically (local and systemic immune responses against cancers) and improved the inherently poor immunogenicity of tumors, enhancing the efficacy of cancer immunotherapy, providing synergistic influences to suppress the progression of tumors, and stimulating abscopal consequences to inhibit metastatic cancer.40 Despite the attractive advantages of black phosphorus, the intrinsic instability may restrict its applications, causing rapid ambient degradation.37,38,41 This can be considered as one of the important challenges for commercial/up-scalable production.42 Compared with these 2D materials, MXenes and MXene-base materials with high hydrophilicity, biodegradability, chemical stability, thermal/electrical conductivity, sorption selectivity, biocompatibility, and antibacterial activity are good candidates in the field of tissue engineering and regenerative medicine.43 Herein, significant recent advancements pertaining to the tissue engineering and regenerative medicine applications of MXenes have highlighted as well as their important challenges and future perspectives.
MXenes can be fabricated by the exfoliation of an “A” layer from a “MAX” antecedent; where M, A, and X represent the transition metal, elements from IIIA or IVA, and N or C, respectively.14,46,50,51 These 2D materials have been produced via different strategies such as the urea glass method,52 chemical vapor deposition,53 molten salt etching,54 hydrothermal syntheses,55 and electrochemical fabrication.56 Bottom-up chemical vapor deposition and top-down wet etching tactics have been broadly developed for the synthesis of MXenes.57 The wet etching technique can be applied to provide MXenes with high quality and the presence of terminations, causing the fabrication of MXenes with a basically hydrophilic nature.58 As an example, Ti3C2Tx MXene fabricated through wet etching Ti3AlC2 was employed for designing a screen-printed electrode, which included a dialysis microfluidic chip for multicomponent direct/continuous monitoring of whole blood.58 Additionally, composite hydrogels have been designed comprising cellulose and Ti3C2 MXene, which are endowed with rapid response near-infrared-stimulated features.59 The fabricated hydrogels loaded with doxorubicin hydrochloride, showed a remarkable acceleration in the drug release, and were employed as multifunctional nanoplatforms for tumor therapy by intra-tumoral injection. In this study, MXenes were fabricated via hydrofluoric-acid-etching (HF-etching) of the aluminum (Al) layers in Ti3AlC2, and then the liquid-phase exfoliation (LPE) in N-methylpyrrolidone (NMP) was performed; the delamination of the prepared bulk Ti3C2 into few-layer ultrathin 2D Ti3C2 MXene nanosheets was accomplished using NMP.59
MXene-based materials can also be fabricated via simple and low-cost approaches (e.g., greener methods and solution mixing) into various shapes and morphologies such as fiber, films, and 3D structures. For instance, Shi et al.,60 have prepared a MXene-chitosan composite via a simple solution technique and utilized the composite for removing Cr(VI) from aqueous solutions. Additionally, Yan et al.,61 reported a greener and eco-friendly method for fabricating reduced Ti3C2Tx MXene nanosheets with improved conductivity, oxidation stability, and surface-enhanced Raman spectroscopy (SERS) activity. They have demonstrated a facile and environmentally benign fabrication of MXene-based materials through L-ascorbic acid treatment at room temperature (Fig. 1).61
Fig. 1 The fabrication process of reduced Ti3C2Tx, including HF etching (a and b), delamination (c), and L-ascorbic acid treatment (d) steps. Reproduced from ref. 61 with permission from The Royal Society of Chemistry. |
Smart biomaterials endowed with unique multiple biologic characteristics are ideally suited for tissue engineering and repair.63 As an example, zero dimensional (0D) Ti3C2 MXene-based quantum dots (QDs) were deployed for immunomodulation to improve post-injury tissue repair wherein their inherent immunomodulatory features selectively reduced the human CD4+IFN-γ+ T-lymphocytes activation while stimulating the expansion of immunosuppressive CD4+CD25+FoxP3+ regulatory T-cells in a stimulated lymphocyte population;63 these 2D structures with BMSC biocompatibility could stimulate fibroblasts derived from pluripotent stem cells. The incorporation of Ti3C2 MXene QDs into a hydrogel from chitosan generated a 3D platform with enhanced physicochemical features for the delivery of stem cells and tissue repair; the hydrogel composite showed good conductivity while preserving injectability and thermos-sensitivity.63 On similar lines, the assembly of biocompatible Ti3C2Tz-enhanced poly(lactic acid) nanocomposite membranes have been realized where n-octyltriethoxysilane mediated the interface of the hydrophobic poly(lactic acid) matrix with the Ti3C2Tz nanosheets;64 the final optimized tensile strength of the produced nanocomposite membrane being 72 MPa, ∼33% higher relative to a pure poly(lactic acid) membrane. Thus, the Ti3C2Tz addition could improve the biological attributes of the membrane comprising the osteogenic differentiation of MC3T3-E1 mouse preosteoblasts, proliferation, and in vitro adhesion.64
The surgical failure and bone-tumor recurrence are of vital concern in view of the residual malignant tumor cells and the lack of bone-tissue integration.65 Thus, the recurrence and bone deficiency in surgical treatment of malignant bone tumors has generated a demand for producing multifunctional therapeutic platforms for efficient tumor therapy and bone regeneration. As an example, the logical incorporation of 2D Ti3C2 MXene with 3D-printing bioactive glass scaffolds has been illustrated wherein simultaneous bone-tumor killing by photonic hyperthermia and bone-tissue regeneration by bioactive scaffolds could be accomplished (Fig. 2).65 The ensuing composite scaffolds had ideal attributes for significant photothermal change of integrated 2D Ti3C2 MXene for stimulating bone-tumor ablation by near infrared (NIR)-triggered photothermal hyperthermia, achieving whole tumor removal from in vivo bone-tumor xenografts. Remarkably, Ti3C2 MXene-integrated composite scaffolds could improve the in vivo growth of newborn bone tissue of the composite bioactive glass scaffolds; such inherent advantages of bone-tumor killing and bone-tissue regeneration may be ideal alternatives for treating bone tumors along with other tissue engineering appliances.65
Fig. 2 The process of Ti3C2-bioactive glass (BG) scaffold (TBGS) preparation (I), bone cancer ablation (PTT in vitro/in vivo) (II and III), and bone tissue regeneration (IV). BGS: bioactive glass scaffold. Reproduced from ref. 65 with permission from Multidisciplinary Digital Publishing Institute (MDPI), (CC BY 4.0). |
Innovative cell-laden bioink encompassing electroconductive Ti3C2 MXene nanosheets were created and uniformly dispersed in hydrogels of hyaluronic acid/alginate, comprising an ideally suited operation for extrusion-based 3D bioprinting.66 The MXenes containing hydrogel bioinks showed remarkable rheological characteristics, permitting the preparation of layered 3D structures with shape preservation and significant resolution. Additionally, these nanosheets within the hyaluronic acid/alginate hydrogels bestowed electrical conductivity to the ink, demonstrating the inadequate electrical conductivity of the present bioinks that misallign with the physicochemical characteristics of the tissue.66 Importantly, the condensed human embryonic kidney 293 cells inked by the MXene nanocomposite showed outstanding cell viability (more than 95%) in both the 3D bioprinted structures and the bulk hydrogel.66 Additionally, smart zero-dimensional biocompatible Ti3C2Tx MXene QDs have been prepared with precise surface changes for subcellular nanomedicine applications;67 they were spontaneously uptaken into human endothelial cells within 24 hours of cell culture and their localization was remarkably stable with no detectable alterations in cellular morphologies. Furthermore, uptaken MXene QDs showed their significant autofluorescence characteristics at various excitation–emission wavelengths to allow post-delivery evaluations; these bioactive and trackable MXene QDs can be utilized as promising nanosystems for subcellular nanomedicine functions.67
Reduced graphene oxide-Ti3C2Tx MXene (rGO-MXene) hydrogel with good hydrophilicity and distinctive inter-linked porous structural design has an unparalleled ability to generate inter-joined three-dimensional networks across three human cell types, namely fibroblast, epithelial adenocarcinoma, and neuroblastoma (Fig. 3).68 It has been observed that the cell attachment to rGO-MXene hydrogel was higher than the rGO-control gel alone. In this study, two hydrogels with unique 3D architectural properties were constructed, comprising, rGO-MXene-centered hydrogel (with additional hydrophilic exterior and was adjudged as softer with elastic moduli of ∼20 kPa) and hydrogel based on rGO (endowed with extra hydrophobic shell and was found to be stiffer having elastic moduli of the order of ∼40 kPa).68 The detectable structural self-assembly was quite reminiscent of neuro-spheres from tumorigenic neuron-like neuroblastoma cells after two days on the hydrogel from rGO, which then de-constructed into diffusive lengthened cellular nets subsequent to seven days of culture. Importantly, the constructions of the ensued platforms permitted nutrient diffusion and stimulated the migration of cells all over the hydrogels.68 The entire assembly of cultured cells showed remarkable inter-pore dispersion competencies, as established via the extensive generation of migration-impelled cell cytoskeleton traits (lamellipodia, filopodia, and neurites). Though the in vitro cellular reactions were consistent on such hydrogels, the histocompatibility outlines undoubtedly varied under in vivo conditions or on assorted tissue types; these hydrogels are promising candidates for organ-on-a-chip technologies.68
Fig. 3 The formation of a unique cellular network guided by heterostructures based on rGO-MXene hydrogels. Immunofluorescent images of cultured cells were obtained (green fluorescent). Reproduced from ref. 68 with permission from Elsevier, copyright 2020 (CC BY 4.0). |
Bone loss or insufficiency is a very challenging issue for the integration of the implant and then functional packing, where evolving biomaterials to supplement bone amount and to regenerate alveolar bone imperfections at the implant site are a critically essential element. To attain innovative inorganic composite materials for the regeneration of bone tissue, MXene can play important and positive roles, especially for the improvement of mechanical features, biocompatibility, and osteoinductivity.64,69 In one investigation, nanocomposite membranes based on 2D Ti3C2 MXene nanosheets and hydroxyapatite nanowires were constructed with promising potentials for bone regeneration.69 The incorporation of ultra-long hydroxyapatite nanowires could improve the mechanical characteristics and the hydrophilicity with an improvement in cell adhesion, proliferation, and osteogenic differentiation. Remarkably, this nanocomposite also displayed optimal mechanical features and biological improvements as this MXene-based nanocomposite membrane effectively improved the bone tissue generation in a rat calvarial bone defect (Fig. 4 and 5).69 In another study, an electrically conductive MXene hydrogel was constructed with varying compositional features, encompassing delaminated 2D fluorescent Ti3C2 nanosheets, bioinspired 3D crosslinked chitosan-based polymeric networks and native 0D fluorescent carbon dots in honey.70 The ensuing hetero-structured Ti3C2-MXene-honey-chitosan entity showed remarkable permeable construction with controlled degradation and preferred swelling traits; the designed composite was significantly biocompatible with various stem cells, with improved cell attachment and survival characteristics. This introduced electroconductive composite was proposed for various tissue engineering applications.70
Fig. 4 The preparation of nanocomposite membranes based on 2D MXene nanosheets and hydroxyapatite nanowires (a). The interaction between ultra-long hydroxyapatite nanowires (UHAPNWs) and MXene, involving the hydrogen bonds generated by terminated surface groups of MXene (–F, O or –OH) and hydroxyl in UHAPNWs (b). Reproduced from ref. 69 with permission from Elsevier, copyright 2020. |
Fig. 5 Bone regeneration performance in the control group, MXene group and ultra-long hydroxyapatite nanowires group (four and eight weeks after the surgery procedure); (a) micro-CT images, (b) surgical process, and (c) radiographic evaluations of bone morphometric factors (bone volume, trabecular thickness, and trabecular separation). Reproduced from ref. 69 with permission from Elsevier, copyright 2020. |
Remarkably, it was reported that 2D MXene with Ti3C2Tx composition could nearly quantitatively adsorb urea (initial concentration, 30 mg dL−1) from aqueous solution and ∼94% from dialysate, the maximal adsorption capacity being 10.4 mg g−1 at ambient temperature.71 The analysis at 37 °C indicated a 2-fold improvement in urea elimination proficiency from dialysate with the highest urea adsorption amount of 21.7 mg g−1. The designed MXene demonstrated suitable hemocompatibility, with no detectable induction of the cell apoptosis and reduction of the metabolizing cell fraction, thus revealing no effects on the viability of the cells at concentrations of more than 200 μg mL−1. Such MXenes with high biocompatibility and improved selectivity for urea adsorption from dialysate can be exploited in constructing miniaturized dialysate regeneration systems for a wearable artificial kidney.71
The electrospun fibers emanating from the polycaprolactone–Ti3C2 MXene composite have been fabricated, and evaluated for their biocompatibility on fibroblast and preosteoblast cell lines;72 they were cell-friendly for both cell lines; however, preosteoblast cells showed better cell viability. Additionally, polycaprolactone–MXene composite electrospun fibers with improved wettability, biomineralization, and protein adsorption have been recommended for wound dressing, bone tissue engineering, and also in cancer therapy (Fig. 6).72 However, additional studies are warranted for the systematic analysis and functionalization of these materials for broadening their applications, especially in the field of tissue engineering and regenerative medicine.
Fig. 6 The process of polycaprolactone–MXene electrospun fiber fabrication and their biocompatibility and cell adhesion study; (a) bio-transmission electron microscopy (TEM), (b) cell viability analysis, and (c) biomineralization process. PCL= polycaprolactone. Reproduced from ref. 72 with permission from Elsevier, copyright 2019. |
Synergistic therapeutic strategies for improving the wound repair procedures by coupling electrical stimulation with the hydrogel dressing have been investigated.73 Multifunctional hydrogels based on regenerated bacterial cellulose and Ti3C2Tx MXene were designed and commissioned for electrical modulation of the cell behaviors for active skin wound healing under external electrical stimulation.73 Consequently, the prepared hydrogels with 2 wt% MXene exhibited the highest electrical conductivity and the safest biocompatibility. Furthermore, these hydrogels exhibited suitable mechanical features, promising flexibility, high biodegradability, and considerable water-uptake capacity. After in vivo evaluation by using a rat full-thickness defect model, it has been noticed that these hydrogels display better therapeutic effects than the commercial Tegaderm film; in vitro and in vivo results showed that under electrical stimulation, the designed composites could remarkably improve the proliferation activity of NIH3T3 cells, and also enhance the wound healing procedure, as compared to non-electrical stimulation controls.73
Interestingly, 2D Nb2C-based MXene showed suitable potential for in vivo photothermal ablation of mouse tumor xenografts in both the NIR-I and NIR-II windows; the ultrathin, lateral-nanosized Nb2C nanosheets exhibited remarkable photothermal conversion efficiency (36.4% at NIR-I and 45.65% at NIR-II), and also noteworthy photothermal stability.75 These nanosheets had promising features, comprising enzyme-responsive biodegradability to human myeloperoxidase, low phototoxicity, and significant biocompatibility (Fig. 7).75
Fig. 7 Ultrathin Nb2C nanosheet fabrication (a), digital photograph (b), SEM images (c and d), HRTEM image of Nb2AlC ceramic bulks (e), digital photograph (f), SEM images (g and h), HRTEM image of multilayer Nb2C (i). Digital photographs (j), bright-field TEM image (k), dark-field TEM image (l), Fourier transform patterns of single-layer Nb2C (m). Reproduced from ref. 75 with permission from American Chemical Society (ACS), copyright 2017. |
In comparison to graphene, MXenes display hydrophilic behavior with considerable conductivity, providing improved interaction with polymeric matrixes, which bodes well for their utilization in composites.46,76 Zhu et al.77 created a soluble 2D MXene (Nb2C nanosheets) system with polyvinylpyrrolidone microneedles for medical implantation and photothermal destruction of superficial tumors in the NIR-II biological window. Herein, 2D Nb2C nanosheets have been utilized as high-functioning photothermal nano-agents while the biocompatible polyvinylpyrrolidone served as a matrix to preserve the needle structures; the ensued microneedle system had enough skin-permeation capability and unique solubility behavior. After being inserted into the skin of the tumor site, they dissolve readily within a short period to release the loaded nanosheets. These microneedle systems showed high biocompatibility with no remarkable toxicity or side effects, and thus can be exploited for minimally invasive, safe and effective localized therapy.77
The evaluation of biological responses of host tissue cells to the MXene bio-interfaces is very significant and especially should be considered for surface coating of the implants.81 As an example, the cytocompatibility of selected MAX phases, Ti3AlC2, Ti3SiC2, and Ti2AlN, have been systematically analyzed, in vitro.82 These phases were found to be anoxic to fibroblasts and preosteoblasts; relative to the strong viable fibroblasts, varied cellular responses for them could be detected for preosteoblasts. It was revealed that under an osteoblastic environment, Ti2AlN had better cell proliferation and differentiation performance than the other surveyed materials. Remarkably, relative to the partly oxidized TiCxOy layer on Ti3AlC2 and Ti3SiC2, the partially oxidized TiNxOy layer on the Ti2AlN showed a remarkable affinity for the Ca2+ ions, and thus useful cytocompatibility of Ti2AlN was detected.82 Another study described that the nanostructured Ti3C2 layer could protect the bulk titanium implant against the harsh conditions of biological tissues as well as stimulate the proliferation, adhesion, and osteoblast activity. Furthermore, it could provide better bone-implant contacts for the implant compared to the uncoated titanium implant.83
Undeniably, MXenes with their unique physicochemical properties have demonstrated several biomedical potentials, especially for bioimaging, biosensing, cancer therapy/diagnosis, antibacterial activity, drug delivery, tissue engineering, and regenerative medicine.84,85 But, for clinical applications of MXenes and MXene-based materials, there are still various challenging issues, especially regarding their biosafety (short-term and long-term toxicity) and their therapeutic efficacy (especially for their potential in synergistic combinational therapy together with conventional strategies) (Fig. 8).86,87 Indeed, these 2D structures for clinical applications should have enough homogeneity and preferably demonstrate suitable and efficient functions with acceptable similarity in patients. Additionally, their clinical potentials are related to their sufficient cytocompatibility/biocompatibility, stability (long-lasting suitable effects), and good biodegradability. These critical properties are affected by some important factors such as size, compositions, surface features, and surface modification/functionalization. An acceptable pharmacokinetic balance should exist between the degradation and stability of MXenes, improving their clinical therapeutic efficacy and reducing possible adverse side effects.81,88
MXenes have great potential for drug delivery/release and can be deployed as efficient platforms for synergistic chemo-photothermal ablation of cancers. However, their controlled and targeted release behaviors should be analytically evaluated and optimized. In one study, an innovative heterostructured nanocarrier based on Ti3C2 MXenes and cobalt nanowires was constructed for synergistic chemo-photothermal cancer therapy with dual stimuli-responsive drug release and magnetic controlling capabilities.89 Additionally, Li et al.90 have constructed a composite nanoplatform using MXenes for synergistic chemo-photothermal therapy of hepatocellular carcinoma. These materials with efficient surface chemistry for targeting engineering have exhibited suitable drug release/delivery capacity, improved hydrophilicity, and acceptable dispersity.90
Future investigations are warranted on responsive scaffolds (both, mechanical and electrical) for regenerative medicine, intracellular fluorescent probes, partition membranes for artificial organs, and implantation of bio-electronic peripheries for neurotransmitter and bio-electronic signal recognition; remarkable electrical and optical features of MXenes render them ideal candidates for sensing applications. Additionally, innovative sustainable and greener synthetic methods for eco-friendly fabrication of MXenes as well as their functional modification for innovative tissue engineering applications need to be explored. There is a vital need to establish the influences of the size and composition of MXenes on their properties and to analyze the interactions between MXenes and drugs or cells; toxicity, biodegradability biocompatibility, histopathological and biosafety issues need to be systematically and analytically evaluated. Suitable biofunctionalization and chemical surface modifications can help to fine-tune various aspects, namely the functionality, stability, bioavailability, biodegradability and biocompatibility, reduce the adverse effects/immune system reactions and increase their targeting property.
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