Issue 32, 2021
From the journal:

Chemical Communications

3-Benzoylquinoxalinone as a photoaffinity labelling derivative with fluorogenic properties allowing reaction monitoring under “no-wash” conditions†

Madeleine Cauwel,a   Clément Guillou,b   Kévin Renault,a   Damien Schapman,c   Magalie Bénard,c   Ludovic Galas,c   Pascal Cosette,b   Pierre-Yves Renard ORCID logo a  and  Cyrille Sabot ORCID logo *a  

* Corresponding authors

a Normandie Univ, CNRS, UNIROUEN, INSA Rouen, COBRA (UMR 6014), Rouen 76000, France
E-mail: cyrille.sabot@univ-rouen.fr

b Normandie Univ, CNRS, UNIROUEN, INSA Rouen, PBS-UMR6270 CNRS, PISSARO Proteomics Facility, IRIB, Rouen 76000, France

c Normandie Univ, Inserm, UNIROUEN, PRIMACEN, Cell Imaging Platform of Normandy, IRIB, Rouen 76000, France

Abstract

Described herein is a quinoxalinone-based photoaffinity probe with caged fluorescence properties. Upon visible blue LED irradiation (λmax 450 nm), this photo-crosslinker is able to covalently capture proteins with concomitant fluorescence labelling. This process enables monitoring applications under “no wash” conditions.

Graphical abstract: 3-Benzoylquinoxalinone as a photoaffinity labelling derivative with fluorogenic properties allowing reaction monitoring under “no-wash” conditions

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Article information

DOI
https://doi.org/10.1039/D1CC01072G
Article type
Communication
Submitted
01 Mar 2021
Accepted
12 Mar 2021
First published
12 Mar 2021

Chem. Commun., 2021,57, 3893-3896

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3-Benzoylquinoxalinone as a photoaffinity labelling derivative with fluorogenic properties allowing reaction monitoring under “no-wash” conditions

M. Cauwel, C. Guillou, K. Renault, D. Schapman, M. Bénard, L. Galas, P. Cosette, P. Renard and C. Sabot, Chem. Commun., 2021, 57, 3893 DOI: 10.1039/D1CC01072G

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