Issue 12, 2020

Chitosan/casein based microparticles with a bilayer shell–core structure for oral delivery of nattokinase

Abstract

Nattokinase is a thrombolytic enzyme obtained from Japanese traditional food natto for prevention and cure of thrombosis-related cardiovascular diseases. However, the effectiveness of nattokinase through oral intake is limited, due to the loss of thrombolytic activity in the acidic gastric juice. In this study, we develop a functional oral delivery system of nattokinase, in which chitosan microparticles were used as the carrier core to load nattokinase via genipin crosslinking and then covered by a casein-based protective shell via transglutaminase (TG) crosslinking. The results of in vitro and in vivo assays, in the aspects of bioactivity, release dynamics, and therapeutic effects, indicated that the bilayer shell–core structure could protect loaded nattokinase from destruction in the gastric juice and achieve its controlled-release in the intestine. This work demonstrates the availability of bilayer shell–core structure design in oral delivery of nattokinase and shows its high potential for application as an anti-thrombosis functional food additive.

Graphical abstract: Chitosan/casein based microparticles with a bilayer shell–core structure for oral delivery of nattokinase

Article information

Article type
Paper
Submitted
05 Sep 2020
Accepted
09 Nov 2020
First published
24 Nov 2020

Food Funct., 2020,11, 10799-10816

Chitosan/casein based microparticles with a bilayer shell–core structure for oral delivery of nattokinase

X. Zhang, X. Lyu, Y. Tong, J. Wang, J. Ye and R. Yang, Food Funct., 2020, 11, 10799 DOI: 10.1039/D0FO02349C

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