Pu Chun
Ke
ab,
Ruhong
Zhou
cd,
Louise C.
Serpell
e,
Roland
Riek
f,
Tuomas P. J.
Knowles
gh,
Hilal A.
Lashuel
i,
Ehud
Gazit
j,
Ian W.
Hamley
k,
Thomas P.
Davis
bl,
Marcus
Fändrich
m,
Daniel Erik
Otzen
n,
Matthew R.
Chapman
o,
Christopher M.
Dobson
g,
David S.
Eisenberg
p and
Raffaele
Mezzenga
*qr
aZhongshan Hospital, Fudan University, 111 Yixueyuan Rd, Xuhui District, Shanghai, China
bARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia
cInstitute of Quantitative Biology, Zhejiang University, Hangzhou 310058, China
dDepartment of Chemistry, Columbia University, New York, New York 10027, USA
eSchool of Life Sciences, University of Sussex, Falmer, East Sussex BN1 9QG, UK
fLaboratory of Physical Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang-Pauli-Str. 10, 8093 Zurich, Switzerland
gDepartment of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
hCavendish Laboratory, University of Cambridge, J J Thomson Avenue, CB3 0HE, Cambridge, UK
iLaboratory of Molecular Neurobiology and Neuroproteomics, Brain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland
jDepartment of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences; Department of Materials Science and Engineering, Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University, 69978 Tel Aviv, Israel
kSchool of Chemistry, Food Biosciences and Pharmacy, University of Reading, Whiteknights, Reading RG6 6AD, UK
lAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Qld 4072, Australia
mInstitute of Protein Biochemistry, Ulm University, 89081, Ulm, Germany
nInterdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark
oDepartment of Molecular, Cellular and Developmental Biology, Centre for Microbial Research, University of Michigan, Ann Arbor, MI 48109-1048, USA
pDepartments of Chemistry and Biochemistry and Biological Chemistry, UCLA-DOE Institute and Howard Hughes Medical Institute, UCLA, Los Angeles, CA, USA
qDepartment of Health Sciences & Technology, ETH Zurich, Schmelzbergstrasse 9, LFO, E23, 8092 Zurich, Switzerland. E-mail: raffaele.mezzenga@hest.ethz.ch
rDepartment of Materials, ETH Zurich, Wolfgang Pauli Strasse 10, 8093 Zurich, Switzerland
First published on 7th July 2020
Amyloid diseases are global epidemics with profound health, social and economic implications and yet remain without a cure. This dire situation calls for research into the origin and pathological manifestations of amyloidosis to stimulate continued development of new therapeutics. In basic science and engineering, the cross-β architecture has been a constant thread underlying the structural characteristics of pathological and functional amyloids, and realizing that amyloid structures can be both pathological and functional in nature has fuelled innovations in artificial amyloids, whose use today ranges from water purification to 3D printing. At the conclusion of a half century since Eanes and Glenner's seminal study of amyloids in humans, this review commemorates the occasion by documenting the major milestones in amyloid research to date, from the perspectives of structural biology, biophysics, medicine, microbiology, engineering and nanotechnology. We also discuss new challenges and opportunities to drive this interdisciplinary field moving forward.
Amyloidosis refers to the accumulation and deposition of amyloid fibrils,15 whose aggregation kinetics contains contributions of both primary and secondary nucleation,16 giving rise to toxic intermediates of oligomers17,18 and protofibrils en route. The amyloid state is characterized by steric zippers of the amyloid cross-β spine,19 and the state is proposed to be accessible by virtually all proteins under physiological or artificial conditions. The crystalline form of amyloid proteins has recently been identified as the absolute free-energy ground state20,21 over the native or amyloid state of proteins. On a mesoscopic scale, amyloid fibrils possess polymorphism, displaying a prevalent yet nonexclusive left-handedness likely originated from the biased chirality of amino acids.22 In engineering, amyloids of whey proteins have found new applications in iron fortification, water purification and in vivo sequestration of pathological amyloid proteins,23–25 while functional amyloid-nanocomposites yield new mechanical, thermal and electronic properties appealing to nanoelectronics, biotechnology and environmental engineering.26
To commemorate five decades of research since Eanes and Glenner's landmark study of human amyloid,1 here we reflect on major milestones in the field of amyloid to date, shared among the three major classes of amyloids: the pathological, functional and artificial amyloids, and we discuss emerging opportunities and grand challenges of the amyloid science moving forward, from the perspectives of basic science, medicine and engineering (Fig. 1).
The widely adopted protein folding funnel model has evolved from both experiment and theory through the use of simplified mechanical models developed by Wolynes, Onuchic, Dill and colleagues35–37 and more recently by Mezzenga and coworkers.20,21Fig. 2 illustrates the folding funnel which is a simplified 2D representation of the very high-dimensional conformational space accessible to a protein during its folding.34 The broad top of the funnel represents a vast number of conformations present in the fully unfolded or stretched state, while the narrow bottom of the funnel depicts the unique native structure of the protein.35 The separation between the top and bottom of the funnel represents other energies (solute enthalpy, solvent entropy and enthalpy) contributing to each protein conformation. However, the chaperone effect (chaperonin-mediated folding) or multi-protein co-folding effect (folding upon binding, etc.) is not included in this picture due to its simplicity.35,36,38 Starting from the ensemble of unfolded conformations, the folding funnel allows many different pathways to proceed rapidly to the global free energy minimum occupied by amyloids, recently refined into a series of closely-positioned local minima, occupied by different amyloid polymorphs, with the amyloid crystals alone occupying the absolute minimum (for an extended discussion on the energetic levels of different amyloid polymorphs see Section 4, “Mesoscopic structures of amyloids and the energy landscape”).20,21 As the chain folds to lower energy conformations, and before reaching the metastable local minima of the various amyloid polymorphs, intermediate states along the sides of the funnel are also populated. During this process, the kinetic traps might hinder or promote formation of native structures depending on their depths and the barriers between the traps and next energy minima. According to statistical mechanics, the number and depth of local kinetic traps on the funnel landscape correspond to the degree of frustration of the protein sequence.35 Following the concept of the folding funnel diagrams, an off-pathway aggregation can be incorporated as second “aggregation funnel”.39 Like intramolecular folding, the association of two or more non-native protein molecules can form an “amyloidosis formation funnel” through intermolecular contacts (Fig. 2). The process is largely driven by hydrophobic forces and primarily results in the formation of amorphous structures (“amorphous aggregates”; Fig. 2).34 Subsequently, aggregation can lead to the formation of amyloid fibrils. These simplistic folding funnel models provide a conceptual framework for understanding the complex process of amyloid formation.20,21,34
Fig. 2 Energy landscape of protein folding and aggregation. The purple surface shows the multitude of conformations ‘funneling’ to the native state via intramolecular contacts and the pink area shows the conformations moving toward amorphous aggregates or amyloid fibrils via intermolecular contacts. Both parts of the energy surface overlap. Aggregate formation can occur from intermediates populated during de novo folding or by destabilization of the native state into partially folded states and is normally prevented by molecular chaperones. Toxic oligomers may occur as off-pathway intermediates of amyloid fibril formation. Reproduced with permission from ref. 34, copyright 2009 Nature Publishing Group.34 |
Meanwhile, recent advances in experimental techniques that probe amyloid formation at different stages have shed light on the nature of both the kinetics and thermodynamics of this complex process (more in the following sections). However, many of the underlying molecular mechanisms and interactions involved in amyloid protein/peptide misfolding and aggregation pathways remain elusive. Computer simulations performed at various levels of complexity ranging from simple lattice models, models with continuum solvent, to all atom models with explicit solvent have been used to offer complementary and valuable insights that cannot be obtained by experimental methods alone.40 In particular, the important role of water molecules in promoting the formation of protofilaments, the basic building blocks of amyloid fibrils, has been investigated using fully atomic molecular dynamics (MD) simulations.41
Although the hydrophobic effect is known to have a significant impact on protein self-assembly in water, the precise mechanism of how it operates as well as the exact role of water in facilitating this assembly remains controversial. In a recent study,41 a model protofilament comprised of two parallel β-sheets of Alzheimer Aβ16–22 peptides (Ac-K16-L17-V18-F19-F20-A21-E22-NH2) was employed to study amyloid formation and the role of water molecules during the process using MD simulation. Each β-sheet presented a distinct hydrophobic face and a hydrophilic face, which together self-assembled into a stable protofilament with a core consisting of purely hydrophobic residues (L17, F19, A21), with the two charged residues (K16, E22) pointing to the solvent (Fig. 3A). The simulation results revealed a subtle interplay between a water mediated assembly and one driven by favorable energetic interactions between specific residues forming the interior of the protofilament. Overall, the role of water during the assembly can be viewed as “lubrication”, namely it does not drive assembly but rather facilitate proper packing of the hydrophobic surfaces in the final stages of the assembly. In some of the MD trajectories, a nanoscale dewetting (or drying) was also observed in which water expulsion preceded hydrophobic collapse, providing a strong driving force for the hydrophobic collapse and hydrophobic patch assembly. This can be attributed to the fact that when two strongly hydrophobic surfaces, greater than 1 nm in length, are brought together to a critical distance, a nanoscale drying might occur between the two hydrophobic surfaces, resulting in a strong hydrophobic collapse.42,43 In the trajectories where no nanoscale drying was observed, water expulsion and hydrophobic collapse occurred roughly simultaneously (i.e., water acting as “lubricant”; Fig. 3).
Fig. 3 (A) Aβ16–22 model protofilament (left panel). The initial structure used to start the MD trajectory with initial inter-β-sheet separation distance D0 of 1.28 nm. Front (a) and side (b) views are shown. Side chains are colored as follows: (K) red, (L) orange, (V) yellow, (F) green, (A) blue, and (E) violet. For clarity, only water molecules in the interpeptide region have been shown. (c) The same structure after 1000 ps of unconstrained MD simulation at 300 K, started from the structure shown in (a) and (b). (d) A single Aβ16–22 peptide pair, one from each layer, is isolated from the protofilament shown in (c). (B) Number of interpeptide water molecules versus interpeptide distance (right panel). (a–d) Plots for each of the four trajectories at 300 K where D0 = 1.28 nm. Trajectories (a) and (b) do not appear to show a dewetting transition, while trajectories (c) and (d) do. (e–h) The peptide–water van der Waals interaction is turned off, and D0 = 2.38 nm. (i–l) The peptide–water electrostatic interaction is turned off, and D0 = 1.28 nm. Reproduced with permission from ref. 41, copyright 2008 American Chemical Society.41 |
The authors also studied the interaction energy decomposition to explore the contributions from various forces.41 Interestingly, turning off the protein–water electrostatic interaction only slightly slowed down the assembly speed without significantly affecting the nanoscale drying (Fig. 3B). Conversely, if the protein–water van der Waals attraction was switched off, a strong dewetting transition and hydrophobic collapse takes place in every simulation (Fig. 3B). These predictions were later validated by experimental (and theoretical) studies of other proteins with large hydrophobic patches.44 Overall, these computer simulations demonstrate that in general, when attractive van der Waals forces exist between the solute and solvent, these forces, though individually small, can be sufficient to compensate for the loss of hydrogen bonds due to the confinement of water between the two plates. However, in extreme cases, such as those highly hydrophobic and rough surfaces in-between the amyloid protofilaments, some nanoscale dewetting might occur which can provide strong driving force for the hydrophobic collapse of amyloid peptides and their subsequent aggregation and fibril formation.45
Fig. 4 X-ray fibre diffraction provides the characteristic cross-β pattern for amyloid. Top panel shows a schematic showing the features of the cross-β pattern and structure. Lower panels show the cross-β diffraction patterns collected from amyloid fibrils formed by a diverse range of amyloidogenic proteins and peptides. Aβ11–25,72–74 AAAKKFFEAAAK,52 silk,75 hIAPP,76 NM Sup35,77 ccβ,78 Met30 TTR,79 Tau,80 Core PHF,81 β2M,82 Aβ42,73,83 GNNQQNY,84 fibrinogen,85 RVFNIM.86 White arrow – major meridional, H-bond spacing: 4.7–4.8 Å; grey arrow – meridional 2.4 Å; black arrow – major equatorial, sheet spacing (10–12 Å; 5 Å for silk). Reproduced with permission from ref. 72, copyright 2000 The American Chemical Society.72 Reproduced with permission from ref. 73, copyright 2003 Elsevier.73 Reproduced with permission from ref. 74, copyright 2000 Elsevier.74 Reproduced with permission from ref. 52, copyright 2005 National Academy of Sciences.52 Reproduced with permission from ref. 75, copyright 2007 Wiley-VCH.75 Reproduced with permission from ref. 76, copyright 2004 Elsevier.76 Reproduced with permission from ref. 77, copyright 2000 American Association for the Advancement of Science.77 Reproduced with permission from ref. 78, copyright 2008 Elsevier.78 Reproduced with permission from ref. 79, copyright 1996 Ciba Foundation.79 Reproduced with permission from ref. 80, copyright 2003 National Academy of Sciences.80 Reproduced with permission from ref. 81, copyright 2017 Elsevier.81 Reproduced with permission from ref. 82, copyright 2008 American Society for Biochemistry and Molecular Biology.82 Reproduced with permission from ref. 87, copyright 2012 American Society for Biochemistry and Molecular Biology.87 Reproduced with permission from ref. 84, copyright 2010 Elsevier.84 Reproduced with permission from ref. 85, copyright 2007 Informa Healthcare.85 Reproduced with permission from ref. 86, copyright 2013 Portland Press.86 |
Transmission electron microscopy was a valuable asset and in 1959, Cohen and Calkins53 provided images of amyloid fibrils extracted from liver. Eanes and Glenner showed the cross-β diffraction pattern from amyloid extracted from liver and spleen1 and then created “amyloid” in vitro from Bence Jones proteins (excreted immunoglobulin light chains, LCs) or their fragments.54 This disease, arising from the formation of LC-derived amyloid fibrils, is now known as AL amyloidosis.55,56 As early as 1946, Waugh reported precipitation of insulin under high temperature, acidic conditions.57 In 1972, synthetic amyloid fibrils were made from insulin by repeated heating and cooling under acidic conditions. This process generated long-straight, unbranching fibrils that resisted degradation58 and gave a cross-β diffraction pattern as well as the characteristic β-sheet signals by circular dichroism (CD) spectrophotometry and Fourier transform infrared (FTIR) spectroscopy. Following this advance, it became possible to create amyloid fibrils from many disease-related peptides such as Aβ,59,60 hIAPP61 and peptides related to larger amyloidogenic precursors such as TTR.62 This paved the way for further structural characterisation to reinforce the description of the amyloid core cross-β structure. The cross-β pattern from ex vivo Val30Met variant TTR provided a model structure, composed of repeating β-strands running perpendicular to the fibre axis and associated to several sheets that twisted with a helical pitch of 115.5°.63 This model was found to be representative of a collection of extracted amyloid fibrils leading to the generic cross-β model for amyloid.64
Atomic force microscopy, transmission electron microscopy and cryo-transmission electron microscopy (AFM, TEM and cryoTEM) provided further macromolecular details and showed that amyloid fibrils were formed of individual protofilaments65–68 and that different precursor proteins may lead to different numbers of protofilaments. It was also becoming clear that synthetic fibrils grown under different conditions could lead to structural polymorphism. For example, insulin fibrils analyzed by cryoTEM showed multiple variations in the number of protofilaments from two to six,69 while Aβ40 was later shown to form even more different classes.70 AFM was instrumental in demonstrating the growth of the fibrils and generally showed that the diameters did not change, but that the growth was additive elongation at the growing ends.71
X-ray fibre diffraction from a short amyloidogenic region of Aβ11–25 gave exceptional detail and these fibrils were also analyzed by cryoTEM to directly visualize the cross-β structure74 (Fig. 4). High-resolution cryoTEM revealed striations that were 4.7 Å apart, reinforcing the previous interpretation from the X-ray data and providing new insights into the stability of the fibrils.74 4.7 Å appeared to be the largest repeating unit and no long-range repeat was apparent. Interestingly, later studies have shown quite considerable variation in the helical twist for fibrils and often very long-range repeats that can vary even within a single filament. This variation goes some way to explaining why longer-range repeats were not observed in diffraction patterns.
Amyloid fibrils are made from a large variety of precursor proteins, ranging from the β-sheet sandwich structures of TTR, immunoglobulin LC and β2 microglobulin, to the α + β structure of lysozyme and the α-rich structure of serum amyloid A.55 Natively unfolded proteins and peptides assemble in diseases such as T2D, AD and Parkinson's disease (PD). Despite this diverse range of starting native structures, all amyloid fibrils share the cross-β structure and all precursor proteins, even those rich in β-sheet, undergo a significant conformational change upon forming amyloid. Early work assumed that antiparallel sheets were formed allowing different length polypeptide chains to access this repetitive structure.72 Intriguingly, electroparamagnetic resonance pointed to a parallel, in-register structure for amyloid fibrils formed from large proteins and this appeared to suggest that the proteins needed to unfold almost entirely to form a layer which then stacked to render the fibrils.88 It seemed improbable, but structural models were put forward showing a β-spine.89 It was not until the first solid-state NMR (ssNMR) models were provided of Aβ fibrils that it was shown that the β-sheets were formed by bending of two strands that stacked to assume a parallel, in register set of β-sheets.90,91 This structure, held together by hydrogen bonding, provided a stack of identical amino acid side chains along the length of the fibrils. It was clear then, that the side chains played an important role in the structure. X-ray and electron diffraction were combined to provide a model for an amyloidogenic novel sequence AAAKKFFEAAAK, showing that the side chains associated across the sheets.52,92
The first atomic-resolution X-ray crystal structures of amyloid-like fibrils, formed in vitro from short adhesive segments of amyloid-forming proteins, revealed the basis of amyloid stability and provided atomic level insights into the amyloid core.19,94 The fibrils are formed from pairs of β-sheets, mated together by interdigitation of their amino acid sidechains. This zipper-like interdigitation of these structures suggested the term “steric zipper” for this motif, which has now been found in numerous X-ray, NMR, and cryoTEM structures19 (Fig. 5). In structures of full fibrils, steric zippers are frequently found at junctions of protofilaments (Fig. 5). Elsewhere in full fibrils, hetero zippers (formed by two different sequences) are found. Factors contributing to amyloid stability include: (1) the hydrophobic effect of releasing water molecules from the tight, dry interface between the sheets; (2) van der Waals stabilization of the interdigitating sidechains; (3) mutual polarization of stacked amide hydrogen-bonding groups parallel to the fibril axis;95 and (4) ladders of stacked sidechains such as the phenolic groups of tyrosine residues on the surface of the fibrils. The steric-zipper motif also explains the sequence specificity of amyloid formation: only compatible sequences can form steric zippers. Peptide inhibitors, designed on the basis of crystal structures of short segments, are effective in inhibiting aggregation and cell entry of full pathogenic amyloid fibrils.96
Fig. 5 Atomic-resolution crystal structures of two adhesive segments and two amyloid fibrils of the protein α-synuclein associated with PD. Upper left: Crystal structure of the PreNAC segment with sequence 47GVVHGVTTVA56 (the first T in this sequence is a hereditary early-onset disease mutation A52T). The upper view is down the axis of this steric zipper, showing atoms with van der Waals radii forming a tight, dry interface. Upper right: Crystal structure of the NACore segment with sequence 68GAVVTGVTAVA78. The center shows two amyloid-like fibrils formed by α-synuclein (αS). In the top of the left center, one layer of the Rod polymorph is viewed down the fibril axis, showing that it contains two identical αS chains each bent into a double hairpin shape. The two chains meet at a steric zipper formed by the PreNAC segments of the two chains. Identical layers are stacked on each other, forming a two-protofilament, slowly twisting fibril. In the top right center, one layer of the twister polymorph is viewed down the fibril axis. The fold of the two αS molecules is similar to that of the Rod polymorph but the two chains meet at a different point than those of the Rod polymorph. They meet at an interface similar to that of the NACore crystal structure. That is, the steric zipper interfaces that pair the β-sheets in the crystal structures are similar to the interfaces between paired protofilaments in the fibrils. Notice that the slowly twisting twister fibril is formed by stacking identical layers on each other, with a slight twist. Reproduced with permission from ref. 93, copyright 2018 Springer Nature.93 |
The technical challenge of determination of X-ray crystal structures of amyloid fibrils is that the crystals are invariably no larger than several microns in size. The hypothesis for the small crystal size is that β-sheets normally exhibit a slow twist but are held in amyloid crystals in untwisted form, producing a strain that builds as the crystal grows, limiting size. In fact, the crystals of the 11-residue NACore segment of αS are only a few hundred nanometers in cross section, and hence invisible by light microscopy. Consequently their structure had to be determined by electron diffraction, for which small crystals are advantageous.93 Yet, as it will be discussed later, the untwisted form of amyloid crystals – compared to the twisted form of their homologue fibrils – opens for a different way to further decrease the overall free energy, placing them at the lowest minimum of the free energy landscape.20
Thanks to developments in ssNMR and cryoTEM,103,104 numerous near-atomic-resolution structures of much longer segments of amyloid fibrils are now available (Fig. 5 and 6). ssNMR yielded structures for a 22-residue fragment of β2 microglobulin,105 Aβ40 by 2008,106 for the more toxic Aβ42 by 2016,97,98 and for αS fibrils.107,108 Further advances in cryoTEM, largely helped by the invention of direct detectors and the treatment of helical structures as single particles109,110 have led to an explosion in atomic detail of amyloid structures. Near-atomic resolution structures were solved for amyloid fibrils of αS,111–113 Tau,101,114 and TDP-43.115 Paired helical filaments and straight filaments from AD brain showed the parallel in register structure with further exciting details at the bends between the sheets. Tau filaments from chronic traumatic encephalopathy patients114 and from Picks disease patients116 show intricate differences that may give us clues regarding the differences between the diseases. Immunoglobulin LC amyloid,117 β2 microglobulin,118 acute phase protein amyloid A (AA)119,120 form similar core structures. Many proteins in the amyloid state are able to assume a variety of structural folds termed polymorphs.
Fig. 6 Example ssNMR and cryoTEM structures for amyloid fibrils. Upper panel: ssNMR structure of Aβ42.97,98 Two S-shaped molecules of Aβ42 (black and gray) are related by a twofold axis (marked by a circle), which runs down the center of the fibril. The N-terminal 14 residues are disordered; one possible conformation is shown here by dotted lines. Many of the known hereditary mutations are carried by residues located on the outer surface (red). The surface hydrophobic patch formed by residues V40 and A42 (orange) may explain the greater rate of secondary nucleation by the 1–42 species compared with 1–40.99,100 Bottom panel: CryoTEM structures of two amyloid fibrils of Tau.101 These two polymorphs of Tau amyloid fibrils were purified from the autopsied brains of AD patients. In both polymorphs, individual Tau proteins form C shapes, as shown by the cartoon ribbons with arrows that lie nearly in a plane perpendicular to the fibril axis. The protein layers are stacked up to form a protofilament. For each polymorph, there are two protofilaments, but they meet at different interfaces. Steric zippers are noted in the straight filament polymorph. The β-helical feature is enlarged in the right-hand panel where it is shown in yellow.102 Reproduced with permission from ref. 100, copyright 2016 National Academy of Sciences.100 Reproduced with permission from ref. 101, copyright 2017 Springer Nature.101 Reproduced with permission from ref. 102, copyright 2017 Annual Reviews.102 |
Despite the variety of molecular structures displayed by amyloid proteins, they show common features. The proteins mainly form extended β-strands, and these are bent into a series of hairpin-shaped β-arches, and are confined essentially in a 2-dimensional slab or “layer”. Backbone amide groups extend their hydrogen-bonding CO and N–H groups up and down, parallel to the fibril axis, and the resulting hydrogen bonds stack the layers into slowly twisting protofilaments (Fig. 5). Most often, two or more protofilaments twist around each other, forming the fibril, but some fibrils are built from a single protofilament and some are formed from several protofilaments. The protein structures remain “cross-β” displaying the expected distance of 4.76–4.78 Å between the hydrogen bonded β-strands which generally run perpendicular to the fibre axis.
Whereas pathological amyloid fibrils tend to be so stable as to be irreversible, considerably more labile amyloid-like fibrils have been found to form from low-complexity domains of proteins that participate in hydrogels and liquid–liquid phase separation.121–123 These low-complexity domains are especially rich in Gly, Ser, and Tyr residues, and poor in most apolar residues. Short segments of these domains have been crystallized and the resulting structures (Fig. 7) are similar to steric zippers in that they show pairs of stacked β-strands. But these weakly adhesive elements differ from steric zippers in that the backbones are usually kinked, with more polar and apparently weaker interfaces that account for the reversibility of the fibrils. These mildly adhesive interfaces have been termed LARKS, an acronym for low-complexity, amyloid-like, reversible, kinked segments. LARKS may contribute to the interactions between proteins with low-complexity domains that participate in transient subcellular bodies, such as stress granules.
Fig. 7 Atomic-resolution crystal structures of five LARKS contrasted with the structure of a steric zipper from the segment with sequence NKGAII from Aβ. The right-hand column shows the paired β-sheets of the steric zipper at the top and of the five LARKS below. For each structure, five layers are shown of the thousands in the crystals, with the fibril axes vertical. The view in the middle column is down the fibril axis and shows all atoms of the interfaces. The view in the left column is also down the fibril axis and shows the tracings of the protein backbones. The tight interface of the steric zipper offers a strong interaction. The kinked interfaces of the LARKS are weaker. Each interface is characterized by its shape complementarity score (Sc = 1.0 for perfect complementarity) and buried solvent-accessible surface area (Aβ) in Å2 between the mated sheets. Nitrogen atoms are blue, and oxygen atoms are red.123 Reproduced with permission from ref. 123, copyright 2018 American Association for the Advancement of Science.123 |
Structural studies of amyloid and amyloid-like fibrils have opened understanding of these pathological and functional architectures at the atomic level. The hope is that continued studies will contribute to the development of diagnostics and therapies for the numerous diseases associated with these fibrils.
The mesoscopic structure of amyloid fibrils has since been extensively characterized, primarily with TEM, cryoTEM and AFM, and similar fibrils have been documented for amyloids and amyloid-like entities including functional and pathological amyloids as well as engineered peptide and protein amyloids of various lengths as short as several amino acid residues, with di-phenylalanine being likely a minimum motif for fibrillization.7,124–126
The repeating 3D structures of these amyloids are composed of many (usually hundreds to thousands) copies of a peptide/protein. As discussed earlier, at the atomic level amyloids are arranged in a one-dimensional ordered cross-β-sheet motif, which consists of two or more layers of intermolecular β-sheets that run along the fibril axis.127 The polypeptides often render unbranched fibrils, 6–12 nm in width and up to several micrometers in length,128 and are in general composed of several protofilaments.129 The protofilaments may twist around each other but not exclusively in a left-handed fashion. Straight fibrils composed of several filaments as well as right-handed fibrils have also been documented, though rarely for the latter.130 The left-handed twist is attributed to the underlying β-sheet secondary structure conformation composed of L-amino acid residues (correspondingly, amyloids composed of peptides of synthetic D-amino acids are usually right handed), although the transfer of chirality of amyloid fibrils across length scales is not conclusively solved, since protofilaments of a given handedness may merge to form mature amyloid fibrils of opposed handedness.131 Irrespective of the final handedness, a full rotation of a filament within a fibril may be in the order of tens to several hundred nanometers requiring a ∼0 to a few degrees of rotation per β-strand. This imposes a limitation on the twist periodicity as the hydrogen bond network of the β-sheet important for the stabilization of the 3D structure is slightly perturbed by the twist.
In general, the twist of amyloid fibrils results from propagation of the chiral β-sheet secondary structure to higher hierarchies, and thus is intrinsically related to the topology of the fibrils; yet extrinsic parameters have also been found to contribute to the overall observed twist. For example, the charged side chains on the fibrillar surface induce a torsion per unit length which is directly proportional to the overall charge. Since the extent of this kind of charge repulsion can be tuned by salt concentration and composition of the buffer medium, the twist periodicity can be manipulated by the salt concentration of the system under study, as demonstrated for β-lactoglobulin first grown at a low ionic strength and then exposed to a high ionic strength post fibrillization.132,133 According to this scenario, the “electrostatic” contribution to the twist can be relaxed by screening electrostatic charges via the presence of salts or buffers, until nearly complete untwist of the fibrils is achieved. The same scenario was observed for the functional amyloid hormone β-endorphin grown in the presence and absence of NaCl: while the fibrils were flat when grown in salt, they appeared twisted when grown in the absence of salt albeit displaying practically the same 3D atomic structure resolved by ssNMR spectroscopy.134
The twist periodicity may vary between fibrils within the same sample and, to some extent, even within a single fibril. In the same sample there may be both straight fibrils as well as twisted fibrils, sheet-like structures, as well as helical ribbons (see below for definitions, as well as Fig. 8). These heterogeneous morphologies are at the core of the so-called “mesoscopic polymorphism”, which arises from distinct structures at the atomic level (also referred to as “molecular polymorphism”),113 distinct protofilament packings,113 local salt concentrations during the nucleation events, or distinct nucleation sites on heterogeneous surfaces. The origin of the polymorphisms is therefore attributed to the (local) environmental conditions, but may also indicate a kinetically trapped origin of the amyloid.135 Nonetheless, the large amount of polymorphism that can be observed at the mesoscopic level, as exemplified by Aβ40, is regarded both a typical property as well as a conundrum of amyloids.
Fig. 8 Structure of amyloid fibrils as a function of the constitutive number of protofilaments in HEWL lysozyme as observed by AFM. The number of protofilaments is indicated in each panel. Up to 3 protofilaments, the fibrils remain straight and in a twisted ribbon configuration (H = 0). Starting from 4 protofilaments, amyloid fibrils change into a helical ribbon configuration, as revealed by the characteristic zig-zag contour shape associated with a non-zero mean curvature (H ≠ 0). Reproduced with permission from ref. 136, copyright 2011 American Chemical Society.136 |
In detail, the mesoscopic polymorphism of amyloid fibrils includes various topologies which can be classified directly by the mean (H) and Gaussian (K) curvatures of the amyloid fibril surface, defined as:
The overall elastic energy per unit length of amyloid fibrils is a complex interplay of torsional and bending energies,20,21 whose contributions change differently with the lateral dimensions of amyloid fibrils. As a consequence, different structures of amyloid fibrils are found as a function of the number of constitutive protofilaments: at a critical width to thickness ratio or for a specific number of protofilaments, a transition from twisted to helical ribbons occurs,136 in analogy to the behavior observed for chiral liquid crystalline films undergoing similar transitions at a critical film width138 or leaves undergoing identical twisted-helical ribbon transition for a critical differential strain.137 In other words, in a twisted ribbon morphology, the twist periodicity itself limits the number of protofilaments per fibril possible, as the outer protofilaments must go a longer way around the central one. A consequence of this fact is that the number of protofilaments per fibril in a twisted ribbon is approximately proportional to the twist periodicity and actually appears to be proportional to the twist periodicity for fibrils with several protofilaments.133,139 Conversely, in a helical ribbon, no universal feature relating the periodicity to the number of protofilaments is observed. This is because helical ribbons can close into nanotubes, thereby reducing the line tension of external protofilaments by virtually maintaining their mean curvature H unchanged and rendering them a metastable precursor to nanotubes. Fibrils composed of two to several protofilaments have been documented. In the case of straight fibrils or helical ribbons, however, the number of protofilaments or ribbons may increase significantly towards a sheet-like entity composed of up to 10 or more filament entities,136,140 with a record-large number of protofilaments in a single flat amyloid ribbon reported for the case of the R3 fragment of Tau protein.141
Recent studies of the elastic energies of twisted ribbons, helical ribbons, nanotubes, flat ribbons and crystals21 have allowed positioning each of these polymorphs in a relative scale of energy (Fig. 9, right panel). Specifically, the absolute minimum in the free energy of the protein folding landscape previously attributed to amyloid fibrils has been refined into a series of relative minima where each polymorph has a specific energy level.20,21 Twisted ribbons occupy a relative minimum in the protein folding energy landscape and must overcome a precise energy barrier to fully untwist and enter the absolute minimum occupied by (achiral, untwisted) amyloid crystals; helical ribbons need to overcome a larger energy barrier to fully untwist and enter the same minimum as amyloid crystals: the extra energy barrier compared to the amyloid twisted ribbons is provided by the twist-bending coupling energetic term existing for helical ribbons but missing for twisted ribbons. Accordingly, no helical ribbon-amyloid crystal transitions have yet been observed, whereas twisted ribbon-amyloid crystal transitions have been well documented.20 Because the energy level of a fully untwisted helical or twisted ribbon is equivalent, this places helical ribbons on a lower energy level than twisted ribbons. Thus, rather than overcoming this larger energy barrier, helical ribbons tend to further evolve by closing into nanotubes, which are further down the energy level reduced by the line tension associated with the external protofilaments found in helical ribbons. Only flat amyloid crystals, for which the translational symmetry associated with a lack of macroscopic chirality accepts reduction of surface tension by lateral aggregation, are allowed to (indefinitely) sink into an energy minimum funnel which is associated with the ground state of the protein folding landscape.
Fig. 9 (left) Schematic representation of the main mesoscopic polymorphs observed for amyloid fibrils and their approximate mean (H) and Gaussian (K) curvatures. (right) Sketch of the protein folding landscape in the region around the amyloid minimum: different polymorphs occupy different energy levels, with amyloid crystals populating the absolute minimum. The right panel is redrawn with permission from Adamcik et al.21 Reproduced with permission from ref. 21, copyright 2018 Wiley-VCH.21 |
A question rises spontaneously of why amyloid crystals which are postulated the ground state in the protein folding energy landscape, are so rarely observed in vivo. As already observed by Adamcik et al.,21 the protein folding process in vivo occurs in non-conservative energy ensembles, with energy injected into and/or dissipated by the system during biological processes and with chaperone proteins assisting protein folding. This is in stark contrast to in vitro processes, where the lack of chaperone proteins and the closed (conservative) ensemble allow revealing the presence of amyloid crystals.
There is a rich history of studies focusing on elucidating the principal features of nucleated polymerization. Much of the early work was carried out in the context of understanding the polymerization of cytoskeletal filaments, including actin and tubulin, which have a similar linear geometry to amyloid fibrils.146 Studies in the 1960s established the principal features of this type of process, including the fact that for early times t the increase in the aggregate mass M follows generically a polynomial behavior M ∼ tn, where n = 2 for simple nucleated polymerization and can have a higher value when the nucleation process is multi-step in nature.146 An important feature of this type of classical nucleated polymerization is that there is only a weak lag phase due to the polynomial time dependence.
Commonly, however, for amyloid formation, the reaction starts with a very marked lag phase during which no or only very low concentrations of aggregates are detected. After the lag phase, the growth and formation of new amyloid fibrils takes place rapidly; this type of process has therefore the features of a highly cooperative transition, where the presence of aggregates facilitates the formation of further aggregates. A central challenge therefore in the mechanistic studies of amyloid formation is to relate the macroscopic observations of protein aggregation to the underlying microscopic mechanisms. A powerful tool in this context is chemical kinetics, a formalism that captures a series of molecular events into a rate law that describes the overall progress of the reaction (Fig. 10A). Application of chemical kinetics to protein aggregation has revealed that in many cases the apparent high level of cooperativity originates from a non-classical secondary nucleation process.16,147,148 In secondary nucleation, the existing amyloid fibrils act as catalytic surfaces for the formation of new amyloid nuclei which can then grow further themselves. This type of process was originally described for crystal nucleation where under many conditions growing crystal faces can favour the formation of new nuclei. Secondary nucleation was also found to be the key process controlling sickle haemoglobin polymerization,149 a non-amyloid related pathological protein assembly process. It has now been identified as a key mechanism for the formation of amyloid fibrils from systems as diverse as Aβ40,147 Aβ42,16 αS148 and hIAPP.150 Recent evidence suggests that the sites for secondary nucleation and growth are distinct and that secondary nucleation takes place preferentially at the sides of amyloid fibrils.151
Fig. 10 Primary and secondary nucleation and their verification with microfluidics. (A) Illustration of the power of chemical kinetics to elucidate microscopic mechanisms. Experimental data for the aggregation of the Aβ42 peptide fitted to an integrated rate law where the dominant source of new aggregates is, from left to right, primary nucleation, fragmentation and secondary nucleation, respectively. (B) Schematic illustration of the microfluidic strategy to detect directly single primary nucleation events and monitor the aggregation reaction in both time and space. (C) Time-lapse microscopy of a single microdroplet trapped in the array shown in panel B. (D) Schematic illustration of the primary and secondary nucleation events and subsequent aggregate multiplication which can be measured directly in microfluidic experiments. Reproduced with permission from ref. 16, copyright 2013 National Academy of Sciences.16 Panels B–D adapted from ref. 145, copyright 2011 National Academy of Sciences.145 |
The existence of secondary nucleation challenges a number of intuitive assumptions about amyloid formation, and perhaps most strikingly that of the nature of the lag phase. Indeed, under conditions where secondary nucleation is a dominant factor, the lag phase is only very weakly dependent on the time to form the initial nuclei, but rather depends on the rate at which these nuclei can grow through elongation and multiply through secondary nucleation.152,153 This observation implies that primary nucleation can be very challenging to study in bulk systems as it has only a very weak effect on the overall kinetics. This picture changes, however, when aggregation takes place in very small volumes, a regime that can be probed through droplet microfluidics (Fig. 10B–D). Microfluidic experiments have allowed the study of single nucleation events, as well as the rate at which amyloid conformations of proteins can propagate in space and time from the site of the original nucleation event.145
The role of secondary nucleation in the development of amyloid diseases remains an active area of investigation. There are indications that this process could be key in generating toxic oligomers that are responsible for neuronal death associated with the aggregation of the Aβ peptide in the central nervous system.154 Indeed, microscopy studies have revealed that the concentration of oligomers is highest in the vicinity of higher molecular weight aggregates such as plaques.155 If the formation of such oligomers was driven by primary nucleation, their concentration would be lowest in the vicinity of plaques as the latter can sequester monomer through their growth, thus leaving less monomer available for primary nucleation. Secondary nucleation, by contrast, is highest in locations which contain both monomer and aggregates,16 in agreement with experimental observations of oligomer localization in vivo. These considerations highlight secondary nucleation therefore as a potential new target for curtailing the accumulation of Aβ oligomers in vivo. Finally, it has become apparent that nature has evolved molecular chaperones that are able to inhibit secondary nucleation in a highly specific and effective manner.154 This inhibition has furthermore been shown to lead to a significant reduction in toxicity associated with protein aggregation, even when the overall concentration of aggregates is not affected, as it significantly reduces the concentration of oligomeric species.
The ability to generate amyloid fibrils in cell-free systems has provided unique opportunities to investigate and dissect the mechanisms of amyloid formation. These studies, performed on Aβ peptides by the Teplow and Lansbury groups,168,169 revealed for the first time that amyloid formation did not follow a two-state mechanism but rather occurred through a series of soluble oligomeric intermediates of variable size and morphologies. The observation that these oligomeric intermediates disappeared upon fibril formation suggested that they were on pathway to amyloid formation. As of today, oligomers have been observed during the fibrillization of nearly all amyloid-forming proteins, suggesting that they are obligate intermediates on pathway to amyloid formation.
Although the great majority of studies have focused on characterizing oligomers that form on the pathway to amyloid formation, increasing evidence suggests that oligomers could also form through fibril-mediated mechanisms or during processes aimed at promoting fibril clearance. Several studies have suggested that oligomers could form during the disassembly or fragmentation of fibrils or upon their interactions with membranes.170–172 The surfaces of fibrils have also been shown to nucleate the formation of oligomers via secondary nucleation mechanisms.173,174 Furthermore, it has also been proposed that amyloid plaques and proteinaceous inclusions may also serve as reservoirs for toxic oligomers.175–179 However, whether oligomers are simply sequestered during the formation of amyloid-rich deposits/inclusions, represent the byproducts of cellular process aimed at dissociating and clearing fibrils, or are formed within these deposits/inclusions remains unknown.
Together, these observations sparked a huge interest in the field because they offered a possible explanation for the lack of correlation between amyloid load and disease onset or severity. This gave rise to an alternative amyloid hypothesis, the oligomer hypothesis, which stipulates that oligomeric prefibrillar intermediates, rather than the amyloid fibrils, are the primary cause of toxicity and cell death in AD, PD and systemic amyloid diseases.
Oligomeric intermediates on pathway to amyloid formation are by definition transient in nature, as already largely discussed above in the context of the protein folding landscape. They do not accumulate and are usually converted rapidly to higher order aggregates, and eventually to fibrils. Although it is possible to capture and detect oligomers during the process of amyloid formation using imaging techniques such as AFM or TEM, isolation of such oligomers during the fibrillization process has proven to be difficult for most proteins. To address this challenge, several protocols have been developed to enhance oligomer formation and/or slow their conversion to fibrils by manipulating solution condition or the use of mutant forms of the proteins that exhibit higher propensity to aggregation (e.g. Aβ42 vs. Aβ40 and variants linked to early onset or severe forms of relevant amyloid disease). Other protocols relied on the use of chemical or radical-mediated cross-linking approaches to trap and/or stabilize transient oligomers to facilitate their characterization or isolation.180–186
At the structural level, on-pathway oligomers tend to exhibit a mixture of secondary structure contents,187–189 often dominated by β-sheet conformations.190 Compared to amyloid fibrils, oligomeric intermediates of most amyloid forming proteins exhibit weak binding to the amyloid specific dyes thioflavin T/S (ThT/S) and Congo red,191,192 suggesting that they have not acquired the cross-β structure that is characteristic of amyloid fibrils, although studies on Aβ oligomers using X-ray fiber diffraction have suggested that some oligomers possess cross-β-like conformations.193 Unlike amyloid fibrils, which despite their polymorphism still share a common core structure, cross-β sheet, amyloid oligomers exhibit large differences in their dynamic properties and structural diversity, suggesting that it is unlikely that one specific molecule or antibody would recognize all types of oligomers formed by one protein. In 2003, Kayed et al. reported that it was possible to generate antibodies that not only recognized different types of oligomers and but also oligomers derived from different amyloid proteins (Aβ, hIAPP, αS and Tau) and suggested that amyloid oligomers derived from these proteins shared common structural features.194 This hypothesis was supported by subsequent findings showing that oligomeric preparations from these amyloidogenic proteins were toxic to cells and neurons. However, subsequent studies by Glabe and colleagues and other groups revealed that the different aggregate and oligomer specific antibodies stained different types of pathological aggregates in the brain and that there was no universal antibody capable of recognizing all type of Aβ oligomers.195–197
The heterogeneity and dynamic properties of the oligomers have thus precluded studies aimed at resolving their structural properties at the atomic level. Oligomers rapidly interconvert between different forms and exhibit high propensity to transition to higher order aggregates, thus making it difficult, if not virtually impossible, to isolate and investigate the structural, functional and toxic properties of a single oligomeric species. Several attempts have been made to achieve this goal, but without any success. The use of sequential chromatography separation methods or other protein separation techniques has enabled the generation of oligomer preparations that are enriched in specific morphologies,169,187,198–201 but generation of homogeneous preparation consisting of one oligomeric species of a defined size and morphology has not been possible. This explains why, despite two decades of active research, it has not been possible to ascribe toxicity to a specific oligomeric entity or develop tools and strategies that target distinct types of oligomers. Furthermore, the diversity of the protocols used to produce oligomers, which leads to oligomer preparations of different size, structure and morphology distribution, combined with the lack of tools and methods that enable precise assessment of oligomer heterogeneity, has made it difficult to compare and reproduce results across different laboratories. Despite these challenges, such oligomer preparations have been used to gain insights into the dynamic properties of oligomers and to elucidate the sequence and structural determinants of oligomer formation and stability using solution and ssNMR, hydrogen deuterium exchange methods and other biophysical techniques.189,202–208
Several assays and methods have been developed to measure the level of oligomers in biological fluids, but the level of these oligomers is usually too low to allow interrogation of their size and conformational properties and thus these studies are usually limited to correlating oligomer concentrations to disease progression. One of the most commonly used oligomer-specific immunoassays is based on using the same antibody to capture and detect the amyloid protein of interest. However, these assays do not differentiate between oligomers and fibrils and may not provide an accurate quantitative assessment of oligomer levels due to the lack of the proper calibrants or calibrants that capture the diversity of oligomers in biological samples.
The past decades have witnessed active development of mitigation strategies against amyloidosis, involving peptidomimetics (1990's onwards),252 monoclonal antibodies (2000's onwards),159 small molecules (2000's onwards)253 and, more recently, organic and inorganic nanoparticles and nanocomposites (2010's onwards) (Fig. 11).22,254 Specifically, peptide inhibitors, such as the β-sheet breaker KLVFF,255 draw inspiration from the structural characteristics of Aβ256 to initiate hydrophobic interaction with the latter and act in a chaperone-like manner. However, enzymatic degradation and poor blood–brain-barrier translocation are the notable undoing of this strategy. Monoclonal antibodies, such as Bapineuzumab (Pfizer, J&J), Aducanumab (Biogen, Eisai), Solanzumab (Eli Lilly) and Ponezumab (Pfizer) targeting the N-terminus, amyloidogenic fragment and C-terminus of Aβ,257–259 failed to pass phase-II/III trials and evoked the question whether amyloidosis inhibition through peptide targeting and clearance is a viable strategy against neurological disorders. Small molecules identified by microarrays,260 such as 2,8-bis-(2,4-dihydroxy-phenyl)-7-hydroxy-phenoxazin-3-one (O4),253 or derived from natural compounds, such as epigallocatechin-3-gallate (EGCG),261,262 ameliorated the toxicities of Aβ and hIAPP in vitro via inhibited (and, occasionally, accelerated) aggregation. A major shortcoming with small molecules, however, is their often limited water solubility and, hence, low bioavailability and poor delivery efficacy.
Nanomaterials and multifunctional nanocomposites are engineered/synthetic structures possessing versatile surface area, functionality and architecture, and can be tailor-designed to alter protein aggregation and match amyloid in size, morphology and physicochemical properties. As a result, amyloidosis inhibition with biocompatible/biomimetic nanoparticles and multifunctional nanocomposites has become an emerging frontier, driven by the rapid development of nanotechnology and accumulating knowledge on nano–bio interactions.254 Simple polymeric nanoparticles, such as dendrimers and star polymers, as well as condensed ceria nanocrystals, graphene quantum dots, graphene oxide, gold nanoparticles, carbon nanotubes, transition-metal dichalcogenide nanosheets (e.g., tungsten disulphide and molybdenum disulphide), multifunctional peptide–polymer nanosweepers, protein–KLVFF–polymers, hIAPP19–29S20G and mesoporous silica nanocomposites, have shown potency in mitigating the amyloidoses and pathogeneses of Aβ, αS and hIAPP in vitro and in AD, PD and T2D animal models.23,263–273 In recent years, it has been increasingly realized that, as with molecular inhibitors, the endpoint of amyloidosis mitigation with nanoparticles is not necessarily inhibition of protein fibrillization per se, but suppression of protein toxicity. Indeed, accelerated protein assembly may reduce the population of toxic oligomers and protofibrils,253,274 analogous to Pmel17 aggregation in melanin synthesis in the skin.275 However, no systematic understanding is currently available to predict whether an exogenous substance, nanoparticles included, inhibits or accelerates protein aggregation, and if accelerated protein aggregation leads to a beneficial or a detrimental effect on cell viability.
Experimental studies of amyloidosis inhibition often involve a ThT or Congo red fluorescence assay to assess the cross-β content and kinetics in protein fibrillization;276 TEM or AFM to characterize the mesoscopic morphology of protein aggregates (see Section 4); CD and FTIR spectroscopies to infer the peptide secondary structure and their time evolution; NMR and beam-diffraction (X-ray crystallography, EM) to reveal the atomic structures of amyloid fibrils (see Section 3); gel electrophoresis, dot blotting and immunohistochemistry to confirm protein–inhibitor binding and amyloid/plaque formation; in vitro assays to quantify cell viability and mitochondrial activity in conjunction with alleviated production of reactive oxygen species (ROS); and in vivo assays to target and clear amyloid oligomers, fibrils and plaques via autophagy,277 improve cognitive function and mobility, and stimulate recovery of gene dysregulation.
Under in vivo conditions, inhibitors may be administered through systemic circulation, or via direct injection into the brain of transgenic mouse models in the cases of AD, PD and HD. The peripheral circulation of nanoparticle inhibitors, for example, may acquire a plasma protein “corona”278 through nonspecific adsorption, which ascribes a new biological identity to the nanoparticle inhibitors to evade opsonization. Here, the design usually aims at extending the circulation and improving the delivery of the inhibitors. Upon binding with amyloid proteins, nanoparticle inhibitors perturb the aggregation kinetics resulting from nanoparticle–protein interactions in competition with protein–protein self-assembly, mediated by hydrophobic interaction, hydrogen bonding and electrostatic interaction. A major indicator of strong amyloid protein–inhibitor interaction is changes induced in the protein secondary structure, which accordingly shift the high β-sheet contents of protofibrils and fibrils to render disordered structures, coils, or alpha helices. While this strategy may be easily demonstrated in test tubes or cell cultures, binding of inhibitors with amyloid proteins occurs far less frequently in vivo due to the much reduced concentrations of amyloid proteins and inhibitors, environmental pH, as well as binding of the interactants with other intra- or extra-cellular proteins, chaperones/chaperone-like proteins (such as serum albumin),279 enzymes, ligands, biometals, membranes and other cellular organelles. Each type of interaction may influence the conformation and/or toxicity of amyloid proteins and impact their associated pathogeneses downstream. Furthermore, it has been shown that hIAPP amyloid fibrils, owing to their strong capacity in initiating hydrogen bonding, can acquire a protein corona in culture medium, enriched mostly by linear proteins and multi-domain proteins of structural plasticity.280 The immune response to corona-shielded amyloids and their precursors, however, remains unclear. The nonspecific amyloid protein–environmental protein association, further complicated by the transient and heterogeneous nature of the toxic oligomeric species (see Section 6) – with the exception of the structurally better defined β-barrels which are unfortunately of a small population281 – implies that morphology-based in vivo recognition of amyloid oligomers, fragments or plaques may be inherently problematic. In addition, the hallmarks of amyloid pathologies, such as inclusions and plaques, are highly heterogeneous enriched by tens to hundreds of types of proteins and metabolites,282–286 including extracellular matrix glycoprotein serum amyloid P (SAP), which is thought to stabilize amyloid from degradation,287–289 and membrane-bound heparan sulphate proteoglycans (HSPGs), which mediate Aβ aggregation and cell uptake.290,291 The in vivo origin, dynamics and mechanisms of such hetero-aggregation and cross-seeding (see Section 8) are largely unknown, posing a tremendous challenge to the design and implementation of amyloidosis inhibitors targeting multiple amyloid proteins.
In addition to the aforementioned strategies, chiral molecules292–294 or nanostructures,295 carbon nanotubes coated by sonicated fragments of whey protein β-lactoglobulin,23 gold nanoparticles coated with milk protein β-casein,296 and polyoxometalate-Dawson derivatives (POMDs) functionalized with histidine-chelating metals (Cu, Fe, Ni, Co and Mn),297 have been utilized as inhibitors against amyloidosis in cell cultures and with zebrafish models (embryos, larvae and adults), exploiting the chirality of amyloid fibrils, protein–metal coordination, chaperone-like inhibition of protein aggregation, as well as functional-pathological double protein coronae mediated by hydrogen bonding and β-sheet stacking. Zebrafish, in particular, have been validated as a high-fecundity alternative to AD, PD and T2D (transgenic) mouse models,298–300 and are especially suited for testing a library of nanoparticle inhibitors to render significant statistics at low cost and high throughput. As multiple abnormalities occur in the homeostasis of essential endogenous biometals,301 cellular delivery or liberation of biometals (e.g., Zn2+ and Cu2+) with functional amyloids24 or metal-binding compounds302 may offer new breakthroughs against amyloid diseases. Although not directly targeting amyloidosis, personalized antisense oligonucleotide (ASO) therapy against a mutation in RNA-binding protein fused in sarcoma (FUS) is being implemented to ameliorate a severe form of ALS,303 a motor neuron amyloid disease. Furthermore, neurotrophic factor (NTF)-based therapies, such as the delivery of cerebral dopamine neurotrophic factor (CDNF), have shown promise in stopping and reversing neurodegeneration.304,305
Fig. 12 Schematic contrasting homologous seeding with heterologous seeding where the seed oligomer (in red) stimulates the growth of oligomers and ultimately fibrils of a different protein or peptide.308 Reproduced with permission from ref. 308, copyright 2013 Morales et al.308 |
Different strains of αS (self-seeded or not) have a differential effect on Tau inclusion in neurons.315 Both Aβ and αS oligomers cause Tau aggregation and lead to the development of neurotoxic Tau oligomers that are rich in β-sheet structure.316In vitro studies revealed that the A53T αS mutant enhanced the fibrillization of both Tau and αS itself.317 It was therefore proposed that such effects may be an important contributor to the heterogeneity in amyloid characteristics and symptoms among different individuals. Using the ThT amyloid fibrillization kinetic assay and EM, cross-seeding of Aβ40 or Aβ42 and αS has been examined.318 The greatest enhancement of fibrillization kinetics was observed for cross-seeding with fibrils of αS.318 Other amyloid forming proteins, such as the DNA-binding protein TDP-43, can cross-seed Aβ and suppress the aggregation of the latter into toxic amyloid oligomers.319 Such oligomers are found in the brains of frontotemporal lobar dementia, pointing to the important role of cross-seeding in pathogenesis in this neurodegenerative disease.319 Cross-seeding has been demonstrated in vivo with Aβ and hIAPP.320,321In vitro studies indicated that hIAPP fibrils are poor seeds for Aβ40 fibrillization (this paper also reported weak seeding efficiency of Aβ40 by a number of other peptides).320 On the other hand, preformed fibrils of Aβ42 were injected into hIAPP transgenic mice leading to hIAPP amyloid formation in the pancreas.321 Co-localization was also observed with hIAPP and pro-hIAPP (hIAPP precursor protein) being co-localized in cerebral and vascular Aβ deposits although in the converse situation, Aβ was not detected in islet amyloid from T2D patients.321 Cross-seeding effects have also been studied for the two different forms of Aβ, Aβ40 and Aβ4287,322 and for point mutants of Aβ40 with Aβ40.320
Cross-seeding of prion proteins from one species to another has been suggested to be a mechanism behind the propagation of specific amyloid strains.323 This has also been observed for yeast prions such as Sup35 where the conformational switch that led to domains rich in glutamine and/or asparagine such as [PSI+] was promoted by heterologous proteins containing a similar domain (as in several other yeast prions) or by overexpression of proteins with prion-like Q-, N- or Q-/N-rich domains.324 Mutual cross-seeding has also been observed for Sup35 with the Rnq1 prion domain protein RnqPD, with extended lag periods compared to the self-seeding processes.325 Hybrid morphologies of Sup35 fibrils sprouting from globular RnqPD structures and RnqpD spherical aggregates coated with Sup 35 seed fibrils were observed.325
Cross-seeding of subunits of insulin (A- or B-chain peptides) with the parent protein was found to be less efficient than self-seeding of the full-length insulin.326 The cross-seeded fibrils had features of the parent insulin protein, the morphology being distinct from that of the seeding peptides. Despite the observed cross-seeding, soluble forms of the A- and B-chain peptides were found to be able to inhibit insulin fibrillization.326 In another study, it was suggested that cross-seeding of hen lysozyme with other proteins was promoted by sequence similarity.327 The ThT fluorescence kinetics were enhanced for other forms of lysozyme compared to unrelated proteins (insulin and α-lactalbumin), even though, for example, hen lysozyme and α-lactalbumin share the same native state fold.327
Following this unexpected discovery, there began an immediate quest to understand its significance, which was initially unclear considering the rather high concentration of phenylalanine required for the self-assembly of amyloid-like fibrils. While the normal concentration of phenylalanine in healthy individuals is only a few tens of μM, there are affected individuals with blood and tissue concentration of phenylalanine in the mM range. The medical condition is known as phenylketonuria (PKU), which is the result of a mutation in the gene coding for the phenylalanine hydroxylase enzyme.330 Individuals affected by the mutation are unable to convert the phenylalanine amino acid into tyrosine. This results in the accumulation of phenylalanine as well as an insufficient amount of tyrosine. Intriguingly, unless treated by a very strict diet, PKU patients show severe neurological symptoms similar to neurodegenerative patients.331 After years of follow-up on PKU patients and close monitoring of the correlation between phenylalanine levels and neurological abnormalities, it is clear that concentrations of phenylalanine above 0.5 mM are strongly associated with severe neurological damage. To validate the relevance of the observed phenylalanine fibrils to the disease, their occurrence in mice model of PKU has been tested. Mice have the same biosynthetic pathway for the production of tyrosine as humans and a mutation in the same enzyme results in the accumulation of the amino acid.332 It was indeed found that antibodies which recognized the fibrillar assemblies, just as in the case of the immunized rabbits, emerged spontaneously in the model mice.7 Moreover, antibodies that recognize the phenylalanine assemblies could allow the detection of phenylalanine deposits in the brains of PKU patients post mortem. These deposits are very similar to the amyloid deposits found in those suffering from neurodegenerative diseases such as AD, and could also be co-stained with the antibodies and Congo red.7 Taken together, these results suggested a typical amyloid etiology in PKU and an extension of the list of amyloid-associated disorders.
Follow-up studies have been conducted to understand the organization of phenylalanine into ordered assemblies and the possible occurrence of oligomeric structures of phenylalanine. Using mass spectrometry, Bowers and co-workers discovered that under physiological pH phenylalanine could form oligomeric structures, in which the phenyl groups were being exposed to the solution.333 This interesting organization could be associated with a tendency to interact with membranes as a result of the exposed hydrophobic patches in the oligomeric form, as has also been predicted theoretically by MD simulations,334 thus explaining the high toxicity of the assemblies. An additional immediate follow-up study by Salmona and co-workers indicates the organization of phenylalanine into order assemblies using small angle and wide angle X-ray scattering as well as AFM.335 Moreover, the researchers show that doxycycline, a known protein amyloid formation inhibitor, hinders effectively the formation of fibrillar assemblies by phenylalanine.335
While phenylalanine accumulation in the case of PKU is well-known, there are dozens of additional medical conditions known as inborn error of metabolism disorders, in which other metabolites are being accumulated.336 In order to test whether the formation of metabolite amyloids in PKU represents a unique case, a larger collection of other metabolites has been screened for the ability to form amyloid-like structures. It was revealed that additional metabolites, including tyrosine, orotic acid, adenine, uracil and cysteine could form fibrillar assemblies with amyloid-like morphology, which bound ThT and Congo red and presented notable cytotoxicity.337 Furthermore, this broader study also demonstrates that the mode of toxicity of these metabolite assemblies, as well as that of phenylalanine assemblies, is by late apoptosis, exactly as in the case of protein and polypeptide amyloids.337 Later studies indicated that additional metabolites, including tryptophan, glycine, and quinolinic acid, could form amyloid-typical fibrillar assemblies in aqueous solutions.338–340
Another interesting property of classical protein amyloids that has recently been discovered is their intrinsic fluorescence in the visible range of the electromagnetic spectrum, as was shown by Kaminsky and co-workers.341 The researchers explain the fluorescence by proton delocalization over terminal hydrogen bonds, which results in the formation of an essentially supramolecular emissive electronic state. Intriguingly, similar visible-range fluorescence has been observed for diphenylalanine nanostructures.342 It has recently been established that metabolite amyloids also possess such fluorescence properties, which also allow their detection in live cells.343 The observed fluorescence of the fluorescence in the context of hydrogen bond networks is also in line with the crystal structure of phenylalanine in its zwitterionic form at neutral pH, as was determined by synchrotron X-ray crystallography in 2014.344 The structure shows a network of hydrogen bonds and π–π interactions, layered with remarkably similar morphology and zig-zag arrangement as compared to protein β-sheet structures. Hydrogen bonds occur between polar layers and the charged moieties of the amino acids, while edge-to-face π–π interactions exist between the charged parts of the layers and parallel displaced π–π interactions between the aromatic side chains.344
Yet another property that is shared by protein and polypeptide amyloids and amyloid-like metabolite assemblies is the generic inhibition by polyphenols and other aromatic compounds.345,346 This common feature is also consistent with the earlier observation concerning the inhibition of phenylalanine fibril formation by doxycycline.335 Additionally, acetylsalicylic acid, which does not affect the organization of protein amyloids, similarly has no inhibitory effect on metabolite fibril formation.346 The metabolite amyloids also exhibit the capacity to interact with membranes similarly to protein amyloids, as determined by a highly-characterized chromatic biomimetic membrane system containing phospholipids and polydiacetylene.347 Also in this sense, the similarity between metabolite and protein assemblies is remarkable.
The aggregation of metabolites appears to be directly linked with neurodegenerative disorders. An intriguing observation is related to the interplay between metabolite assemblies and the induction of protein amyloid formation in the context of seeding. It has been shown that phenylalanine fibrils could seed a large group of amyloidogenic proteins and polypeptides that are associated with amyloid diseases.348 It was suggested that the presence of metabolite seeds could have a triggering effect on the eruption of amyloid-associated neurodegenerative disorders.349 Indeed, it was shown that quinolinic acid, a well-established early marker of PD, could seed the aggregation of αS into amyloid fibrils.340 This may provide the missing link between the metabolite profile and the development of various degenerative processes and may clarify certain unexplained epidemiological associations.349
All of the experiments in metabolite amyloids until 2019 have been performed in vitro or in cell culture. It was recently demonstrated that a yeast model of metabolite aggregation could be constructed.350 By blockage of adenine salvage pathways, a yeast model in which adenine accumulates was obtained. Feeding these model yeast with adenine exerted a toxic effect in a non-linear sigmoidal-shaped dose-dependent manner.350 Moreover, the addition of polyphenol inhibitors of amyloid rescued the yeast cells without lowering the level of adenine, indicating that the formation of aggregates, rather than the presence of a high concentration of the metabolite, resulted in the cytotoxicity.350 As protein amyloid models in yeasts have been extensively explored by many groups flowing the pioneering work of the late Susan Lindquist,351 the current use of yeast models allows to compare the cellular mechanisms of protein homeostasis (proteostasis) and that of metabolite homeostasis (metabostasis). The yeast system will allow further analysis of the cellular machinery that is involved in the response to abnormal accumulation of metabolites with and without self-assembly into amyloid-like structures.
Finally, another interesting parallelism can be found between protein and metabolite amyloids in the sense that as with protein amyloids, functional assemblies that are composed of metabolites can be formed.352 One key example is the formation of tapetum lucidum, a retroreflector layer that facilitates night vision. These assemblies are formed by simple metabolites that are very similar to major human risk elements, including a nucleobase (guanine) in reptiles, an amino acid (cysteine coordinated with zinc) in dogs, and a vitamin (riboflavin) in cats and lemurs. Other studies indicated the materials-like properties of amino acid assemblies and the piezoelectric properties of amino acid crystals.353 The overall organization of the metabolites in those systems seems to be related to metabolite amyloids, highlighting once again the similarity between the protein and non-proteinaceous self-assembling systems.
Taken together, the spectrum of amyloid building blocks appears to include not only proteins, polypeptides and short peptides, but also a large number of metabolites (Fig. 13). The full molecular determinants that facilitate the ability to form amyloids are still unclear as very similar metabolites could either form or not form amyloid-like structures. For example, phenylalanine and tyrosine readily form amyloid-like structures, whereas the structurally similar 4-hydroxyphenylpyruvate does not. Additional work should be performed to fully understand the structural, functional, and pathological significance of these new type of non-proteinaceous amyloidal assemblies.
Several types of systemic amyloidosis can be distinguished depending on the fibril precursor protein. This dependence is reflected by the disease nomenclature.56 Systemic AA amyloidosis arises from the misfolding of SAA protein. Systemic AL amyloidosis involves fibrils from immunoglobulin LCs, while systemic ATTR amyloidosis originates from TTR fibrillization. There are several commonalities among the different types of systemic amyloidosis. The precursor proteins are either constitutively or transiently present at high concentrations.354,356 They are proteolytically truncated in the fibrils,354,356,357 and their deposits are associated with non-fibril components, such as glycosaminoglycans,358 lipids359 or non-fibril proteins,360 that are conserved across different diseases and possibly important for fibril formation358 or fibril stability in the tissue.355
Precisely 60 years after Cohen and Calkins revealed, with electron microscopy, the presence of fibrils in the tissue,53 cryoTEM structures became available for amyloid fibrils that were purified from the tissue of patients affected by systemic AA, AL or ATTR amyloidosis.120,361–363 These structures revealed that the fibril protein folds are, in all cases, substantially different from their conformation in the respective native proteins. The implication of this observation is that the conformation of the native state has to be almost entirely unfolded in order to enable the reorganization of the polypeptide chain into the fibril protein fold. This conclusion holds even in cases where the fibril precursor is β-sheet rich as in TTR.362 The fibrils that can be extracted from a patient or animal are typically polymorphic with one dominating fibril morphology.120,361–364 Consistent fibril morphologies are deposited at different sites/organs of the same patient or animal365 and even in different patients or animals, as long as they belong to the same subtype of systemic amyloidosis and possess the same sequence of the fibril precursor protein.119,362,365
Current ways of treatment of systemic amyloidosis are mostly focused on a reduction of the fibril precursor protein. Treatment in systemic AL amyloidosis typically involves a chemotherapy to remove the plasma cell that produces the amyloidogenic LC.356 Treatment in systemic AA amyloidosis involves anti-inflammatory treatments to reduce the serum levels of the acute phase protein SAA.354 Treatment of systemic ATTR amyloidosis can involve a liver transplantation to remove the main TTR-producing organ.366 In addition, there are recent advances to downregulate the expression of TTR protein by RNA interference therapy.366 These treatments, which target the precursor protein concentration, have to be combined, in some patients, with the removal of the compromised organ. An example hereof is the requirement of heart transplantation in many patients with cardiac amyloidosis.356 Finally, certain forms of systemic ATTR amyloidosis can be pharmacologically treated with the drug tafamidis, which binds into the surface pocket of TTR, stabilizing the native state of this protein.367 In summary, systemic amyloidosis represents an interesting and unique model system for studying the principles of protein misfolding diseases and for developing new medical approaches.
A critical component of bacterial biofilm is the extracellular matrix (ECM) that surrounds and protects cells. In many cases, the ECM is strengthened by bacterially produced amyloid.373,374 The best-studied example is curli,375–377 produced by Enterobacteriaceae, such as Salmonella and Escherichia coli (E. coli), as well as hundreds of diverse Gram-negative bacteria.378 Unlike pathological amyloids, bacterial amyloids are functional (i.e. biologically useful) and therefore produced as the result of highly coordinated biosynthetic processes. Much can be learned about the nature of amyloid formation by studying this fascinating group of proteins.
Curli are required for proper biofilm development376,379–381 and enhance biofilm strength, viscoelasticity, and resistance to strain.382 The main component of curli, CsgA, is a 120-residue IDP that is secreted into the extracellular milieu as an unfolded monomer and was the first ‘functional’ amyloid protein to be described.383,384 The minor curli subunit CsgB, also an IDP, nucleates CsgA fibrillization as an amyloid template.385 Additional CsgA subunits are added to the growing fibril end in a seeding process.386,387 CsgB also helps anchor the fibrils to the membrane surface via CsgF (in whose absence both proteins simply “escape” from the cell). Both CsgA and CsgB must be “chaperoned” to suppress their high aggregation propensity and promote their transport as monomers to and across the outer membrane prior to amyloid fibril formation. They are secreted to the cell surface through what is called the type VIII secretion system (Fig. 14), in which CsgE targets them to the outer membrane secretion channel CsgG388–391via the two proteins’ N-terminal 22 residues.392,393 Once polymerized, fibrils can interact with the dyes Congo red and ThT, leading to a spectral change that is often used to monitor amyloidogenesis. The resulting CsgA fibrils are unusually durable. Not only do they resist heat, proteases, and denaturing agents such as sodium dodecyl sulfate (SDS),394 they only dissociate to monomers at very high concentrations (>50%) of the potent denaturant and solvent formic acid.395
CsgG is comprised of nine identical subunits, each lipidated at the N-terminal cysteine residue to promote localization and assembly in the outer membrane (Fig. 14).389,391 Each CsgG subunit within the membrane-embedded CsgG homononamer contributes four β-strands to a 36-strand transmembrane β-barrel, which sits atop a ring of globular periplasmic domains. The structure contains two vestibules separated by a series of constriction loops that restrict the pore diameter to 9 Å.390,396 The first vestibule, formed by the periplasmic domains, encloses ∼24000 Å3, roughly the predicted volume occupied by a single unfolded CsgA monomer. The second vestibule formed by the β-strands spanning the outer membrane remains permanently open to the extracellular milieu, enclosing ∼41000 Å3. This is consistent with the need for subunits to be largely unfolded during secretion into the extracellular space.397 Besides binding and inhibiting the folding of CsgA, CsgE also forms a functional nonameric cap that binds to the CsgG pore and prevents unregulated transport across the outer membrane.388,390,398 CsgE contains a mix of three stacked β-strands, 2 α-helices, and a prominent IDR.398 Once CsgA is confined to the space formed by periplasmic vestibule of CsgG and the CsgE cap, it is thought that the resulting entropic free energy gradient favors CsgA expulsion into the extracellular space. CsgF and CsgB are also apparently secreted through CsgG. A partial NMR structure of CsgF suggests it to be conformationally dynamic with exposed hydrophobic regions. CsgF contains an N-terminal unstructured region, a 21-residue α-helix, and a C-terminal antiparallel 4 β-strands.399
According to “fishing expeditions” with amyloid-specific antibodies, many bacteria produce amyloid,400 and numerous operons coding for functional amyloid have been discovered since curli (see an overview in ref. 401). Despite differences in detail and independent evolutionary origins, there are common features. These include one main amyloid component and a secretion system to transport them to the outer membrane in a monomeric state that is ready for extracellular assembly. For example, the fap operon in Pseudomonas species directs the formation of fibrils mainly consisting of FapC,402 assisted by the (likely) nucleator FapB and transported through the outer membrane by FapF.403 The ensuing fibrils increase biofilm rigidity and resistance to drying out through increased hydrophobicity.404 However, given their independent origins, there will always be distinguishing features in each system. For example, the membrane-embedded part of FapF is a trimeric porin gated by a helical plug (much simpler than CsgG), while its periplasmic coiled-coil domain could potentially guide transport of FapC to the FapF channel as a dedicated “amyloid shunt”.405
The core of both CsgA and CsgB protein sequences is composed of 5 imperfect repeated sequences (R1–R5), each of around 20 residues.375 Peptides corresponding to R1, R5 and R3 assemble into amyloid fibrils that are morphologically indistinguishable from those produced by WT CsgA,406,407 whereas R2 and R4, which are largely dispensable for fibril formation in vivo,406 do not fibrillate on their own. This indicates that CsgA consists of multiple amyloid-forming units, and indeed a combination of NMR data and secondary structure predictions suggest that each repeat sequence in CsgA forms strand-loop-strand motifs.393,408,409 Subsequently co-evolutionary analysis combined with computational force field analysis led to an atomic-level structure prediction in which the 5 hairpins stack on top of each other to form an α-helix,410 although the handedness of this helix still is unresolved in the absence of experimental restraints. The repeats in CsgA are distinguished by the sequence Ser-X5-Gln-X4-Asn-X5-Gln;393,406 the Gln and Asn residues in R1 and R5 are critical for efficient amyloid formation393,406,411,412 while “gatekeeper” residues in R2, R3 and R4 slow CsgA amyloid formation both in vivo and in vitro.411 The structural convergence of CsgA and CsgB is rooted in amino acid sequence similarities, including regularly spaced Ser, Gln and Asn residues.413
A similar situation is found with FapC, although it differs from CsgA in that it contains 3 longer imperfect repeats (∼35 residues), separated by linkers of variable length. A computational-bioinformatic structure prediction, similar to that performed for CsgA, suggests that the FapC repeats form hairpin loops, while the linker regions constitute disordered regions that may lead to a “hydrophilic halo” around the fibril.414 The advantage of this repeat-based design, both for FapC and CsgA, is that each protein monomer constitutes an amyloid-forming unit, which will facilitate rapid initiation of fibrillization. No stable oligomers of either CsgA or FapC have been isolated (except when assisted by fibrillization inhibitors such as polyphenols415,416). This indicates that the repeat structure efficiently drives fibrillization from a small and stable nucleus and that there are no competing non-fibrillar structures, unlike e.g. the stable αS oligomer.417,418 Consistent with this, fibrillization of both CsgA and FapC follows the simplest possible model, namely primary nucleation followed by elongation.419 This mechanism is ideal for the nucleation-initiated elongation of bacterial amyloid fibrils in vivo, where long (i.e. not fragmented) fibrils are likely required to strengthen the ECM. Remarkably, step-wise deletion of the repeats of FapC gradually increases the tendency of the fibrils to fragment, and this means that elongation of the new growing ends generated by fragmentation (rather than elongation of the nuclei formed by primary nucleation) becomes the dominant contribution to fibril growth.420 The resulting fibrils are also more prone to dissociation in formic acid, a direct indication of their reduced stability, and complete removal of all repeats, while not preventing formation of β-sheet rich fibrillary aggregates, catastrophically destabilizes the fibrils in terms of sensitivity to denaturants such as formic acid and surfactants.421 A further twist on in vivo fibrillization is that biomolecules in the milieu close to the outer membrane of Pseudomonas, such as the biosurfactant rhamnolipid and the outer membrane component lipopolysaccharide, strongly accelerate FapC aggregation and suppress accumulation of a transient aggregation intermediate.416 Thus, conditions have truly been optimized to facilitate rapid and direct fibrillization of these proteins.
Amyloidogenic proteins present significant challenges to the cell because of their strong tendency to aggregate. Uncontrolled amyloid formation can upset proteostasis, and is associated with significant cytotoxicity.422 Therefore, a fundamentally important question is: “How can aggregation-prone proteins such as CsgA be guided from the inside of the cell, through the periplasmic space, and across the outer membrane so that amyloid fibrils only form outside the cell?” The answer is: accessory proteins such as CsgC, a small periplasmic protein made by curli-producing bacteria that helps discourage intracellular amyloid formation by CsgA and CsgB. Purified CsgC can inhibit amyloid formation at extremely substoichiometric 1:500 (CsgC:CsgA) molar ratio, both for WT CsgA, R1, R3 or R5 CsgA deletion mutants, along with synthetic peptides corresponding to R1 and R5. CsgC also inhibits amyloid assembly of E. coli CsgA and CsgB homologs, but not the AD-associated protein Aβ42, suggesting some specificity.423 However, CsgC does inhibit amyloid assembly by the PD-associated protein αS,423 possibly due to a 8–9 amino acid motif (D-Q-Φ-X0,1-G-K-N-ζ) shared by both CsgA and αS.423 Structural homologs of CsgC are found in almost all bacteria.424 The CsgC structure can even be seen in the human amyloid-forming protein TTR. Like CsgC, TTR can discourage CsgA and αS amyloid formation.425 Therefore, it is likely that nature has many anti-amyloid chaperone proteins at its disposal that can help modulate amyloid formation.
Functional amyloids have much to offer going forward. Amyloid-associated diseases affect an estimated 50 million people annually. We need better approaches for combating diseases such as AD and PD, which are twice as prevalent today as they were 20 years ago, a number that could quadruple by 2050. The rules governing amyloid fibril formation have been mostly defined through in vitro observations, because most disease-associated amyloids aggregate inefficiently or sporadically in vivo. It is not unreasonable to expect that next generation amyloid therapeutics will be inspired by the mechanisms that functional amyloid systems employ to temporally and spatially control amyloid formation. At the very least, the N22-based display system can be circumvented to target other amyloidogenic proteins to the CsgG secretion channel and provide a bacterial selection system for e.g. aggregation inhibitors.426 Furthermore, the robust fibrils produced by these bacteria may serve both as selectable display vehicles for e.g. peptides with different binding properties427 and even as components in underwater glue when fused with mineral-binding proteins such as mussel foot proteins.428
The extreme aspect ratio of amyloid fibrils provides unique opportunities for assisting the self-assembly of materials templated by liquid crystalline interactions, for example. This has allowed the design of 2D materials for optoelectronic and biosensing applications, among others. The general strategy followed is that the 1D excluded volume interactions of amyloid fibrils can be exploited to drive the self-assembly of other higher dimensionality anisotropic objects, such as 2D organic and inorganic materials, starting at concentrations of the 2D objects well below their intrinsic isotropic–nematic transition composition. In other words, 2D objects can be ordered in their dilute, isotropic state, when amyloid fibrils above their isotropic–nematic transitions are present. In one example, amyloid fibrils above the isotropic–nematic transition were used to drive the alignment and stacking of graphene nanoflakes into layered structures and finally 2D nanocomposites with unconventional sensing, shape-memory and biodegradable properties.431 This offered the first opportunity to design amyloid-based devices for enzymatic biosensing.431 Similarly, replacing graphene nanoflakes with gold 2D single crystals allowed generating hybrids materials with innovative optoelectronic properties, conductivity and plasmonic properties.432 In a further example, inorganic gold single crystals were replaced by ad hoc synthesized 2D hydroxyapatite platelets, which once aligned by excluded volume interactions into layered composites were able to mimic bone structure, with amyloids replacing collagen fibrils.433 The further growth of osteoblasts onto these 2D hybrid materials brought the templates one step closer to mimic real human bones.433 In the dense phase, liquid crystalline interactions of amyloid fibrils were further used to design solid films with anisotropic absorption and fluorescence properties;434 furthermore, although not directly demonstrated, the role of highly anisotropic interactions and structure in amyloid-based films may lead to improved quantum efficiency of semiconductive polymers when embedded in amyloid solid matrices,435 as extensively demonstrated by the Inganas group,436–438 among others.
In the liquid state, the liquid crystalline interactions of the amyloids combined with their chiral nature have been recently shown to be responsible for the formation of chiral nematic phases, or cholesteric liquid crystals, in both a continuum (bulk cholesteric phase) and dispersed state (i.e. cholesteric droplets dispersed in a continuum isotropic phase).439 In both the solid and liquid states, the concepts discussed above can open the path to optical devices where the anisotropic nature of the amyloid-based materials can be used to alter the orientation of an electromagnetic field, as in filters for polarized fluorescence and low-power liquid crystal displays, or the collective diffusion of excitons or photons, in photovoltaic and organic/polymer light-emitting diode (OLED/PLED) applications, respectively.
An alternative way to exploit the extreme aspect ratio of amyloid fibrils is to generate gels, rather than liquid crystals, where the high aspect ratio is now used to reach a gel phase at extremely small percolation thresholds. This can be done by simply altering the interactions among amyloids fibrils in the colloidal state, by for example favoring attractive interactions via changes in ionic strength (charge screening) or pH (alteration of linear charge density).440–442 Amyloid-based gels of this type have been shown to work as efficient scaffolds for cell culture and growth, due to a noteworthy low cytotoxicity in the greatest majority of cases investigated.443–446 Remarkably, this spans over a wide range of amyloid building blocks, ranging from the 10-residue 105–115 fragment from the protein TTR,444,445 modified and engineered to carry the RGD integrin binding sequence of fibronectin, to the much simpler amyloid gels based on food proteins, such as lysozyme.447,448 These gels have been demonstrated to be efficient means for cell proliferation, to mimic the ECM environment, to perform tissue engineering, and, when stem cell precursors are used, to promote cell differentiation, as in the case of neural progenitor cells differentiating into mature neurons during cell growth.449 Of particular significance in this case is the fact that small variations in pH, ionic strength and concentration can result in gels with significant changes in mechanical and rheological properties.450 This is understood to be the playground in which stem cell differentiation in vitro and in vivo occurs, and has been demonstrated to date in several types of amyloid-based gels of different amyloid sources, including peptides based on Aβ451 and αS.452
The amino acid chemistry decorating the surface of amyloid fibrils further enables unique applications, some of which are highly specific to these systems. Indeed, the presence of peptide bonds opens for both versatile covalent and supramolecular chemical approaches. Examples of covalent grafting of organic moieties to amyloid fibrils include PNIPAM brushes grown from β-lactoglobulin amyloid fibrils to produce injectable temperature-responsive hydrogels;453 photo-induced cross-linking of the lipase enzyme onto amyloid fibrils to produce biocatalysts with enhanced enzyme stability;454 and DNA–amyloid conjugates in which DNA provides the possibility of origami-driven colloidal assembly of amyloid fibrils.455 Supramolecular interactions between metal ions and amyloid fibrils have been shown to be particularly promising for applications. Metal–ligand supramolecular binding is at the origin of the strong adsorption of metal ions observed on the surface of amyloid fibrils, mediated by strong binding to a multitude of amino acids (see Fig. 15 for a schematic cartoon).25,456,457 By exploiting this effect, Bolisetty et al. were able to produce amyloid-based membranes for adsorption of heavy metals and radionuclides for efficient water purification.25 Due to the simultaneous presence of diverse amino acids and thus, a multitude of metal–ligand pair binding constants, these membranes act as “universal sponges”, challenging the highly specific exchange resins traditionally used in water purification, for example. This has led to a commercial application of membranes based on artificial amyloids capable to remove a wide range of heavy metal ions such as gold, platinum, silver, copper, lead, mercury, uranium, aluminium, and even metalloids such arsenic.25,456,458
Fig. 15 Schematic illustration of the binding process of gold ions with the β-sheets of amyloid fibrils. The β-sheet structure is based on the molecular dynamic simulations of the self-assembly process of the LACQCL sequence, a fragment found on the β-lactoglobulin amyloid fibrils used for water purification properties.25 Image rights: R. Mezzenga. |
The concept has further been extended to remove halogen contaminants from water, such as in the case of fluoride, by first capturing transition metals on the fibrils, then growing them in situ into fine transition metal nanoparticles, which finally, by adhering to the amyloid surface, are capable of binding targeted fluoride contaminants.459
The growth of metal ions on the surface of amyloids into metal nanoparticles conjugated to the amyloid fibrils can be further exploited for applications as diverse as heterogeneous catalysis460 and human nutrition. For example, by growing iron oxide nanoparticles from Fe2+ bound on the surface of β-lactoglobulin amyloid fibrils, Shen et al.24 showed that the resulting hybrids maintained iron at the right oxidation state (i.e., the II form, which is more bioavailable than the III form), which could then be used to target iron deficiency in vivo. The authors further demonstrated that the β-lactoglobulin amyloid fibril–iron oxide nanoparticle hybrids could be used as efficient iron fortificants for nutrition purposes, with outstanding bioavailability features. Alternatively, by growing noble metal ions such as gold and palladium into nanoparticles, and using β-lactoglobulin amyloid fibrils as a sole reducing agent, the ensued metal nanoparticle–amyloid hybrids could be used to design membranes for continuous heterogeneous flow catalysis.460
The scope of artificial amyloids can be expanded significantly by combining some of the unique features discussed above: amyloid gels can be combined with the reducing properties of the amyloid fibrils to template gold aerogels with ultra-light density;461 the reducing properties of amyloids can be used to generate other types of oxide nanoparticles, such as TiO2 for dye cell units for energy conversion;462 engineered amyloid fibrils with specific binding sites can be used to target analytes and specific molecules; for example, the Eisenberg group463,464 has shown how amyloid templates can be used for efficient CO2 adsorption, thereby addressing another pressing environmental issue. It is the multitude of features highly specific to amyloid fibrils, which, when exploited synergistically, can give rise to an extremely vast range of beneficial applications ranging from the biomedical field, to environment preservation, energy management and alike, serving modern society and shifting paradigms away from the adverse tinge that amyloids have been mostly known for.
Amyloidosis occurs across different organs and biological barriers in vivo, as exemplified by systemic ATTR, AL and AA amyloidoses. On the other hand, amyloid proteins such as Aβ, αS and hIAPP have been detected in systemic circulation, fuelling the hypotheses of cross-seeding, inflammation, the gut microbiota and metabolite amyloid as causative to amyloid diseases.5,477–479 Furthermore, Aβ oligomers have shown a negative effect on constricting human capillaries in AD via signalling to pericytes.480 Accordingly, the structure and toxicity profiles of amyloid proteins may be examined across different compartments, physiological conditions and pathologies.
A major challenge in the clinical treatment of amyloidosis is to ensure early diagnosis of the disease. In T2D, for example, beta-cell mass accounts for only 1–2% of the pancreas but is a key indicator of the onset and severity of the disease. Accordingly, the development of molecular imaging probes and biomarkers based on antibodies/nanobodies, RNA sequencing and multimodal detection is an identified area of clinical importance.481–483 Aside from technological development, and in addition to solubility and efficacy in delivery, the dose and timing of therapeutics administration are crucial parameters for the treatment of amyloid diseases. This is because amyloid proteins in vivo undergo conformational changes on timescales vastly different from in vitro test conditions, depending upon the age, mutation, anatomy and body mass of the animal models. Fundamentally, this challenge arises from the transient and highly heterogeneous nature of toxic oligomers and protofibrils, compounded by cell membranes, pH, ligands and chaperones to influence the protein aggregation energy landscape and toxicity. The evolution of amyloid fibrils in physical and toxicological properties484 adds another element of complexity for amyloidosis inhibition.
It should be noted that significant progress has been made in recent years with regard to the clinical diagnosis and effective management of systemic AL and ATTR amyloidoses. This is achieved by mass spectroscopy identification, chemotherapy, liver transplantation, gene sequencing, small-molecule (e.g., tafamidis and diflunisal) stabilization, antisense oligonucleotide therapy as well as RNAi to mitigate the production or misfolding pathways of amyloid proteins.485–487
Much remains to be understood concerning the structure–function–pathogenesis relationship of amyloid proteins and their structural derivatives, as well as the co-aggregation and cross-seeding of amyloid proteins with environmental proteins. A large body of experimental evidence, particularly from genetics, indicates that the proteins associated with amyloidosis are the central player in pathogeneses. The toxicities of the oligomers and protofibrils are indisputable facts of amyloid proteins. Amyloid plaques first observed by Alzheimer in 1907 and their cross-β structure first revealed by Eanes and Glenner a half century ago,1 are a major culprit for a wide range of debilitating human diseases which remain to be deciphered and, hopefully, eradicated in the coming decades.
Amyloids in current science and technology have evolved significantly beyond their original strictly pathological roles, taking up a novel original profile and illuminating new opportunities which were unthinkable only two decades ago. Two new classes of amyloids have emerged, the functional amyloids and the artificial amyloids, performing challenging and remarkably important roles in vivo as well as in modern nanotechnologies. The unique structural and physicochemical properties shared by pathological, functional and artificial amyloids, have evolved from the debilitating role of the former class in neurodegenerative diseases, to the beneficial tasks played by the two latter classes of amyloids, with a demonstrated and emerging role in modern technologies, and entailing a wealth of applications in environmental remediation, health, composite nanomaterials, energy devices, biosensors, soft matter and 3D printing.26,428–430,488
Footnote |
† This article is dedicated to the memory of our dear colleague Sir Christopher Dobson. Chris was an exceptional scientist, scholar, and a true pioneer of the field of amyloid studies as well as a wonderful mentor to his colleagues and students. He has inspired a whole generation of scientists and enriched the lives of so many people. He was widely admired and will always be remembered for his formidable contributions to the field of Chemistry, his sharp wit, and also his kindness and generosity. |
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