Efren
Andablo-Reyes
a,
Demetra
Yerani
a,
Ming
Fu
a,
Evangelos
Liamas
a,
Simon
Connell
b,
Ophelie
Torres
a and
Anwesha
Sarkar
*a
aFood Colloids and Bioprocessing Group, School of Food Science and Nutrition, University of Leeds, UK. E-mail: A.Sarkar@leeds.ac.uk
bMolecular and Nanoscale Physics Group, School of Physics and Astronomy, University of Leeds, UK
First published on 11th October 2019
Biocompatible microgels have been demonstrated to act as excellent lubricants, however, the influence of the continuum on their overall mechanical performance has been neglected so far. In this work, the mechanical performance of colloidal whey protein microgels (hydrodynamic diameter ∼100 nm measured using dynamic light scattering and atomic force microscopy) of different rigidity dispersed in Newtonian (buffer and corn syrup) or complex non-Newtonian fluids (xanthan gum) is investigated for the first time via rheology and soft tribology. Dispersions of both soft microgels (G′ ∼ 100.0 Pa) and hard microgels (G′ ∼ 10.0 kPa) were observed to act as thickeners in buffer as well as in low viscosity corn syrup and correspondingly reduced the friction, latter decreased as a function of the increased rigidity of the microgels. Differently, in high viscosity continuum, the microgels acted as thinning agents and increased the friction. In the lubrication limit, microgels in buffer or corn syrup behaved as Newtonian fluids with effective viscosity corresponding to their second Newtonian plateau value (η∞). However, the lubrication performance of the microgels dispersed in the complex fluid (xanthan gum) could not be described quantitatively by η∞. For the low viscosity xanthan gum, the microgels had no influence on friction. Nevertheless, for the high viscosity counterparts, the soft microgels acted as thinning agents whilst the hard microgels accelerated the onset of elastohydrodynamic regime. This study demonstrates that microgels act as viscosity modifiers directly influencing the tribological performance, depending upon a subtle interplay of rheological properties of the particles and continuum.
Aqueous lubrication has been recognized as one of the main mechanisms for bio-lubrication due to the abundance of water in complex living organisms.10 Aqueous lubrication is attributed to the capacity of biopolymers to electrostatically bind water molecules while being tethered to the bio-surfaces. Hydrated layers formed near the surfaces by this mechanism are capable of supporting high pressure such as those developed in synovial joints.11 The aqueous lubrication performance of colloidal microgels has recently attracted research attention, such as in the case of particles made of thermo-responsive hydrogels12 or biopolymers including starch,13 whey protein14,15 as well as non-starch polysaccharides (alginate,16 agarose,17 κ-carrageenan18). The capacity of these soft particles to reduce friction in soft mechanical contacts has been attributed to their action as surface separators in combination to a roll bearing mechanism, the latter phenomenon is still under debate.13
An important aspect which has been as-of-yet neglected in the literature, whilst possibly having an exceptional relevance in the mechanical performance of the colloidal dispersions, is the role of the continuous phase i.e., the continuum in which the microgels are dispersed.19 Farres et al. carried out an elegant study where the role of co-solutes, such as glucose and glycerol on the lubrication properties of agar fluid gels was investigated.20 The authors proposed that altering both the rate of ordering transition and the degree of solvation of the gel particles by the co-solutes present in the continuum could influence the lubricating behaviour of fluid gels. Nevertheless, many, if not most studies conducted on microgel or fluid gel lubrication have focused on simple aqueous dispersions. Complex fluids are, in general terms, a combination of a variety of microscopic components with different microstructures, such as colloidal particles and polymers. The self and collective dynamics and configuration of these components contribute in different manner to the macroscopic behaviour of the material.21 For instance, the response of complex fluids to mechanical deformation is dominated by a wide spectrum of relaxation times associated with different levels of component level reorganisation, such as chain segments decorrelation22 or colloidal mesostructures breakage under shear.23 The understanding of the mechanical behaviour of microgel dispersions in complex continuum might also be relevant as simple aqueous media are often altered with the addition of rheology modifiers in real-life applications. Particularly such fundamental knowledge will open up possibilities for templating new soft microgel + complex continuum combinations with optimized performance, such as shear-thinning with ultra-low friction coefficients, and will pave the way towards the development of novel functional biomedical, personal care and nutritional applications.
In this work, colloidal whey protein particles are investigated as models of soft microgels dispersed in continuums of different rheological behaviours. Whey protein microgels have been recently regarded as important biocompatible lubricants.14,15 Two different types of well-characterised fluids are used as continuum in this study. On the one hand, corn syrup solutions with different viscosities are used as Newtonian continuum to serve as a comparison with various literature. On the other hand, xanthan gum solutions are investigated as examples of complex non-Newtonian continuum. While the lubrication performance of corn syrup has been found to be controlled by the viscosity24 of the Newtonian fluid, the lubrication capacity of xanthan gum has also been largely attributed to aqueous lubrication.25,26 In addition to the changes in the continuum properties, the role of the particle softness was also investigated by synthesising microgels of different whey protein content to influence the corresponding protein-crosslinking densities. Variation of mechanical characteristics of microgels and continuum allowed establishing a relationship between the high shear rheology of the dispersions and their lubrication properties, which to the best of our knowledge has never been reported for this kind of complex continuum before.
The previously crosslinked whey protein gels (10.0 wt% or 15.0 wt%) were mixed with either phosphate buffer or corn syrup solutions at a 1:1 w/w ratio and subsequently sheared with a hand blender (HB711M, Kenwood, UK) for 1 minute. The whey protein gel dispersed in either the phosphate buffer or the corn syrup was then placed under vacuum for 30 minutes in order to remove the excess air introduced during blending. The system was finally homogenized via the Leeds Jet Homogenizer (University of Leeds, UK), with two passes at 300 ± 20 bars.14,27 The final concentration of microgels in either the buffer or the two corn syrup solutions was 50.0 vol%, although the protein content in the microgels was either 10.0 or 15.0 wt%. Volume fraction calculation was based on gravimetric measurement based on previous literature,14 however future work should consider calculating volume fraction of the particles based on theoretical models on soft-hairy colloidal spheres.28
In order to ease the discussion of results, labels are given to the different samples according to the content of protein in microgels and the type of continuum (i.e., buffer, corn syrup or xanthan gum solution) as described in Table 1.
Sample name | Microgel protein content (wt%) | Corn syrup content (wt%) | Xanthan gum content (wt%) |
---|---|---|---|
Buffer | No particles | 0.0 | 0.0 |
Buffer10 | 10.0 | 0.0 | 0.0 |
Buffer15 | 15.0 | 0.0 | 0.0 |
50CS | No particles | 50.0 | 0.0 |
50CS10 | 10.0 | 50.0 | 0.0 |
50CS15 | 15.0 | 50.0 | 0.0 |
75CS | No particles | 75.0 | 0.0 |
75CS10 | 10.0 | 75.0 | 0.0 |
75CS15 | 15.0 | 75.0 | 0.0 |
0p5XG | No particles | 0.0 | 0.5 |
0p5XG10 | 10.0 | 0.0 | 0.5 |
0p5XG15 | 15.0 | 0.0 | 0.5 |
1XG | No particles | 0.0 | 1.0 |
1XG10 | 10.0 | 0.0 | 1.0 |
1XG15 | 15.0 | 0.0 | 1.0 |
Topographic images were acquired using a Bruker Multimode 8 AFM equipped with a Bruker Nanoscope V controller. Silicon nitride AFM cantilevers (model MLCT-DC-BIO) with a nominal spring constant of 0.01 N m−1 were purchased from Bruker AFM probes (Camarillo, CA). These cantilevers are thermally stable despite the low spring constant, so it was possible to maintain the force set-point at extremely small values, typically <100 pN producing the most defined images, whilst at 150 pN sample deformation was clearly observed. Slow line rates of 0.5–0.8 Hz and gains at the very upper limits of stability were necessary to track the microgels with minimum disturbance. The measurements were performed at room temperature, using a fluid cell loaded with 20 mM phosphate buffer solution (pH = 7). Images were acquired at 768 pixel resolution and processed using Bruker Nanoscope Analysis v1.9. To our knowledge, this is the first study that has carried out AFM imaging of whey protein microgels.
Particle size distribution was also calculated by processing the topographic AFM image with Nanoscope Analysis v1.9 and represented as histograms for microgels containing 10.0 (Fig. 2a) or 15.0 wt% (Fig. 2b) protein. Size distributions obtained by AFM are in good agreement with DLS measurements regarding the hydrodynamic radii of the microgels. The particle size distributions of microgels with 10.0 wt% and 15.0 wt% protein content dispersed either in buffer or 75.0 wt% corn syrup solution are presented in the Fig. S1 (ESI†). The viscosity of the continuum that has been used during microgel formation did not influence the particle size significantly.
(1) |
Fig. 3 Shear viscosity of Newtonian and non-Newtonian fluids used as continuum in microgel dispersions. Buffer (dashed line), 50CS (filled square) and 75CS (filled triangle) are Newtonian fluids with shear viscosity independent to the shear rate . 0p5XG (open square) and 1XG (open triangle) are shear thinning non-Newtonian fluids. The continuous lines correspond to fittings using eqn (1). |
Sample | η ∞ (Pa) | μ (U = 0.005 m s−1, boundary*/mixed lubrication) | μ (U = 0.04 m s−1, mixed lubrication) |
---|---|---|---|
Buffer | 0.0007 | 0.67 ± 0.08* | 0.41 ± 0.06 |
Buffer10 | 0.004 | 0.48 ± 0.1* | 0.20 ± 0.05 |
Buffer15 | 0.010 | 0.23 ± 0.06 | 0.09 ± 0.01 |
50CS | 0.008 | 0.89 ± 0.18 | 0.18 ± 0.03 |
50CS10 | 0.010 | 0.36 ± 0.09* | 0.09 ± 0.02 |
50CS15 | 0.022 | 0.14 ± 0.07 | 0.023 ± 0.005 |
75CS | 0.070 | 0.07 ± 0.05 | 0.0049 ± 0.006 |
75CS10 | 0.022 | 0.26 ± 0.05 | 0.04 ± 0.02 |
75CS15 | 0.041 | 0.07 ± 0.02 | 0.009 ± 0.001 |
0p5XG | 0.008 | 0.22 ± 0.08* | 0.09 ± 0.03 |
0p5XG10 | No data | 0.31 ± 0.04 | 0.14 ± 0.03 |
0p5XG15 | 0.12 ± 0.05 | 0.05 ± 0.01 | |
1XG | 0.034 | 0.08 ± 0.03 | 0.020 ± 0.008 |
1XG10 | No data | 0.17 ± 0.03 | 0.080 ± 0.004 |
1XG15 | 0.06 ± 0.01 | 0.032 ± 0.004 |
In order to appreciate the impact of microgel inclusions on the overall properties of the dispersions, their relative viscosity (ηr) was plotted as function of the Peclet number (Pe) as defined in eqn (2) and (3), respectively.
(2) |
Pe = 6πηca3/KBT | (3) |
The role of the microgel properties is further explored by increasing their whey protein content to 15.0 wt%. Viscoelasticity of the parent whey protein gels was studied before shearing them into microgels in order to have an estimate of the mechanical properties of microgels. The Fig. S2 (ESI†) shows the linear elasticity of the parent whey protein gels. The elastic shear modulus of the whey protein gels at 10.0 wt% and 15.0 wt% protein content were about 0.1 and 10.0 kPa, respectively. Therefore, the microgels containing 10.0 and 15.0 wt% protein in this study have been referred to as soft and hard microgels, respectively. Dispersions of microgels with 15.0 wt% protein content show differences in the shear thinning region. Sample Buffer15 exhibits similar thinning in comparison to Buffer10 with second Newtonian plateau starting at similar value of Pe ∼ 0.5. Interestingly, relative viscosity of Buffer15 shows a twofold increase in comparison to the Buffer10 in the plateau region. The increase in viscosity can be associated to relatively higher elastic modulus of microgels with higher protein content. Here again microgels in Buffer15 appears to act as thickening agents increasing the viscosity relative to the continuum. Samples 50CS15 and 75CS15 show a similar thinning region between them extending towards Pe values higher than the experimental window. However, values of relative viscosity exhibited by these two fluids are considerably higher in comparison to the rest of the experimental fluids in the shear thinning region with Pe < 2.0. Thus, their response in the shear thinning region at low Pe numbers splits the microgel dispersions studied here into two groups depending on both, particle rigidity and continuum viscosity. This might be because the definition of Pe does not consider particle deformability during flow. Fitted curves to the experimental data are shown in the Fig. S3 (ESI†), where absolute viscosity is presented as function of shear rate.
Fig. 5 shows the relative shear viscosity of microgel dispersions in xanthan gum as a function of shear rate. Due to the non-Newtonian nature of the continuum, it is not possible to define a single Brownian diffusion time, thus the use of Pe was not suitable to describe the balance between the hydrodynamic and Brownian relaxation. Fig. 5 shows a detrimental effect of microgel with 10.0 wt% (0p5XG10 and 1XG10) protein content on the shear viscosity relative to the continuum in the whole range of shear rates studied here. The decrease in viscosity is higher in sample 1XG10 in comparison to 0p5XG10 due to the higher viscosity of the continuum in the former. Both samples started to show an onset of a plateau region at shear rates above 200.0 s−1 with ηr about 0.4 and 0.6 for 0p5XG10 and 1XG10, respectively. Differently, microgels with 15.0 wt% protein content exhibit either neutral or active role in the dispersion viscosity depending on the shear conditions and continuum properties.
Relative viscosity of 0p5XG15 is about 1.0 up to shear rates of 1.0 s−1, increasing afterwards to reach value of about 2.0 at 100.0 s−1. Interestingly, 1XG15 shows values of ηr as high as 10.0 for shear rates up to 100 s−1. After this point, ηr decreases monotonically reaching a value of about 4.0 at the shear rate of 1000.0 s−1. In comparison to the rest of the dispersions, the behaviour of 1XG15 is counterintuitive since the higher viscosity of the continuum should contribute to decrease ηr values. This behaviour is evidence of a synergistic effect between microgels with 15.0 wt% protein content and the 1.0 wt% xanthan gum mesh. Further studies are necessary to understand this inversion. In the case of dispersion in xanthan gum continuum, shear viscosity do not show the onset of the second Newtonian plateau making it not possible to fit eqn (1) to the experimental data.
It is important to mention that all rheological discussion has been carried out under the assumption of stick conditions where the macroscopic deformation (imposed at the sample-rheometer surface interface) is representative of the deformation at any point of the fluid bulk. However, wall slip is known to be an artefact present when studying highly concentrated dispersions of Brownian solid and soft particles.34 Noteworthy, that microgel dispersions studied here exhibit a fluid-like behaviour, where transmission of strain/stress from the rheological surfaces to the fluid bulk is mostly performed through the continuum making the chances of slippage to be highly unlikely. Furthermore, stress curves are shown in the Fig. S4 and S5 (ESI†) for microgel dispersions in Newtonian and non-Newtonian continuum, respectively. As it can be observed, shear stress increases monotonically showing no signs of discontinuities suggesting no obvious wall slippage. Overall, the rheological analyses are intended to provide an explanation to the tribological performance of the microgels presented in the next section.
Fig. 6 Friction coefficient as a function of U (a, c and e) or η∞U (b, d and f) for microgel dispersions in Newtonian continuum. (a and b) Buffer (squares), Buffer10 (circles) and Buffer15 (triangles). (c and d) 50CS (squares), 50CS10 (circles) and 50CS15 (triangles). (e and f) 75CS (squares), 75CS10 (circles) and 75CS15 (triangles). The particle volume fraction of microgels is fixed at 50.0 vol% in all systems. Continuous line in b, d and e represents an average Stribeck curve of Newtonian fluids (buffer and corn syrups) used in this study. Continuous lines in c and d are fittings to the hydrodynamic lubrication regime using eqn (4). Error bars represent standard deviations. |
Fig. 6c presents the Stribeck curves obtained for microgels in 50 wt% syrup solutions (50CS10 and 50CS15) as well as the continuum alone (50CS). Stribeck curves for 50CS and 50CS10 showed the boundary and mixed lubrication regimes. Sample 50CS15 showed the mixed lubrication regime and the onset of the elastohydrodynamic lubrication regime. The latter is observed as an upturn of the Stribeck curve with friction coefficients increasing with the entrainment speeds above 0.1 m s−1. At U = 0.005 m s−1, friction coefficient obtained for 50CS10 is about 0.36, twice as low in comparison to CS50 (plain continuum) at the same entrainment speed. While at U = 0.005 m s−1, 50CS is found to be working in the boundary regime (friction coefficient independent of speed), 50CS10 is working in the mixed lubrication regime showing a subtle decrease in friction on increasing entrainment speed. 50CS75 is also found to be working in the mixed lubrication regime with friction coefficient about 0.14, approximately four and two times lower in comparison to 50CS and 50CS10, respectively. This confirms the positive influence of increasing whey protein in microgels, decreasing the friction coefficient as observed also for microgels dispersed in buffer (Fig. 6a). In Fig. 6b, at entrainment speed U = 0.04 m s−1, 50CS, 50CS10 and 50CS15 performed in the mixed lubrication regime. While values of friction coefficients for 50CS and 50CS10 are not significantly different (p > 0.05), the friction coefficient measured for 50CS15 was significantly lower in comparison to both, 50CS and 50CS10. Moreover, at approximately U = 0.1 m s−1, the onset of the hydrodynamic lubrication can be clearly appreciated only in the curve corresponding to 50CS15.
In the case of simple Newtonian fluids, increasing lubricant viscosity decreases the speed at which the elastohydrodynamic lubrication regime starts.24 This can be clearly observed comparing 50CS (Fig. 6c) and 75CS (Fig. 6e), both Newtonian fluids having viscosities of 0.008 and 0.07 Pa s, respectively. Due to its larger viscosity, 75CS displays the elastohydrodynamic lubrication, while in the same range of speeds 50CS shows only the mixed regime. Applying this reasoning to dispersions 50CS10 and 50CS15 in Fig. 6c, the appearance of an elastohydrodynamic regime for the latter indicates larger viscosity in comparison to the former.
In Fig. 6e, Stribeck curves correspond to 75CS and microgels dispersions 75CS10 and 75CS15. Opposite to the observations in dispersion in lower viscosity continuum (i.e., Buffer10, 50CS10), microgels in 75CS10 increased friction in the mixed lubrication regime in comparison to the continuum alone (75CS). For instance, at U = 0.005 m s−1, friction coefficient found for 75CS10 is about 0.26, representing more than a three-fold increase with respect to 75CS. 75CS and 75CS15 show similar lubrication, however the onset of elastohydrodynamic lubrication reveals differences in viscosity. Onset of elastohydrodynamic lubrication of 75CS appears at the lowest speed at about 0.03 m s−1, followed by 75CS15 at 0.07 m s−1 and finally 75CS10 shows the end of mixed lubrication (and start of elastohydrodynamic regime) at a higher speed of about 0.2 m s−1. Thus, in agreement to discussion presented above, viscosity values of the lubricants follow the same order from highest to lowest, i.e., 75CS > 75CS15 > 75CS10. This is also in line with values of viscosity obtained by means of steady shear rheological experiments as shown in Table 2. The appearance of the hydrodynamic lubrication regime in friction curves for samples 50CS15, 75CS and 75CS15 makes it possible to have an estimation of effective viscosity from a tribological perspective. de Vicente et al.,35 provided an arithmetic expression for the soft-hydrodynamic lubrication regime of a rolling/sliding ball on plate contact as introduced in eqn (4):
μ = 1.46Ū0.65−0.70 + SRR(3.8Ū0.71−0.76 + 0.96Ū0.36−0.11) | (4) |
A common procedure to compare the tribological performance of different fluids is accomplished by using their rheological properties representing friction coefficient as function of the product of viscosity and entrainment speed. In the case of Newtonian fluids, this procedure is well known to deliver master curves (overlapping of curves representing different lubricants) that covers at least the mixed and hydrodynamic lubrication regimes, where fluid dynamics plays an important role in the lubrication.35 Although for most non-Newtonian lubricants the viscosity is a function of working conditions (i.e., shear rate), a similar representation is commonly found where the Newtonian viscosity is replaced by a value of viscosity at a high shear rate (above 1000.0 s−1).25 This approximation relies on the fact that the tribological limit imposes high shear rates on the lubricant due to the proximity between contact surfaces involving submicron separations. Here, the relation between lubrication performance of non-Newtonian microgel dispersions and their rheology is carried out by representing the friction coefficient as function of the product of entrainment speed and η∞. In fact, the successful fitting of eqn (4) in the elastohydrodynamic lubrication regime is a clear indication that this procedure is adequate at least for microgels in Newtonian continuum. In the case of lubricants 75CS10 and 75CS15, the plateau was not observed in the experimental window and values for η∞ were estimated by extrapolation using eqn (1). Fig. 6b, d and e show friction coefficient as function of the product U·η∞ for microgel dispersion with Buffer, 50CS and 75CS as continuum, respectively. A Stribeck curve representing the average lubrication performance of Newtonian lubricants is also presented for comparison purposes. There is a reasonable overlap of Stribeck curves for dispersions regardless of their continuum and protein content in the microgels. Hence, lubrication performance of colloidal microgel dispersions in media with different viscosity (covering three orders of magnitude) can be approximated by considering them as Newtonian fluids having viscosity η∞. In other words, tribological deformation corresponds to the rheological shear rates in the second Newtonian plateau of the microgel dispersions, which has been seldom reported in literature. It is worth noticing that lubricants 50CS15 in Fig. 6d, 75CS10 and 75CS15 in Fig. 6f, show a clear overlap in the onset of the transition between the mixed and hydrodynamic lubrication regime. This is an important indication that hydrodynamic forces acting on the microgel dispersions in the soft tribological limit are still dominated by the rheological response in the equivalent shear rate range, i.e., high shear rate limit.
Having established the influence of microgels in Newtonian continuum of different viscosities in the rheological and tribological limit, a similar study was also conducted for the non-Newtonian continuum, i.e., xanthan gum solutions. Fig. 7a shows the Stribeck curves obtained for microgels dispersions 0p5XG10 and 0p5XG15 in comparison to their continuum alone (0p5XG). Stribeck curves for all lubricants shown in Fig. 7a show only the mixed lubrication regime. In Fig. 7a, only 0p5XG15 shows significant differences. For instance, at entrainment speed 0.005 and 0.04 m s−1, friction coefficient values for 0p5XG15 are about half the values found for 0p5XG and 0p5XG10 at the same speeds. Fig. 7b shows the Stribeck curves for microgels dispersed in the highest viscosity non-Newtonian continuum (1XG). While Stribeck curves for 1XG and 1XG15 show no relative differences, friction coefficients obtained for 1XG10 are significantly higher in comparison to the other two curves up to U = 0.1 m s−1, where a minimum for the friction coefficient is reached. The appearance of a hydrodynamic lubrication regime is only evident in the case of 1XG15, where fitting of eqn 4 generates a value of viscosity of about 0.2 Pa s. Using this calculation, a comparison between rheological and tribological performance can be carried out for 1XG and 1XG15. Effective viscosity of 1XG15 in the elastohydrodynamic regime (eqn (4)) represents a six-fold increase in comparison to the high shear viscosity value extrapolated for 1XG (Table 2). However, in the mixed lubrication regime, no significant differences are found between friction coefficients of 1XG and 1XG15. Thus, it is clear that a single value of viscosity η∞ cannot be used to explain the lubrication performance of microgels in non-Newtonian continuum (xanthan gum). In addition, in the non-Newtonian continuum, microgels in the lowest viscosity continuum (0p5XG) can improve the lubrication with respect to the continuum and the same microgels can cause a detrimental effect when dispersed in the higher viscosity continuum (1XG). Regardless of the continuum properties, lubrication performance of the microgel dispersion is better for the higher protein content particles.
Fig. 7 Friction coefficient as a function of U for the microgel dispersions non-Newtonian continuum. (a) 0p5XG (squares), 0p5XG10 (circles) and 0p5XG15 (triangles). (b) 1XG (squares), 1XG10 (circles) and 1XG15 (triangles). The particle volume fraction of microgels is fixed at 50 vol% in all fluids. Continuous lines in b represent fitting to the hydrodynamic lubrication regime using eqn (4). Error bars represent standard deviations. |
Using the definition of relative viscosity, data presented in Fig. 8 is divided in two regions representing the influence of microgels as thickening or thinning agents. In the thinning region, relative high shear rate viscosity is lower than 1.0, with microgels decreasing the viscosity with respect to the continuum. Whether a dispersions falls in either regime depends on both, continuum viscosity and the mechanical properties of the microgel particles.
This is true for both, Newtonian and non-Newtonian continuum. Based on a balance between stored (in soft elastic particles) and dissipated (by Newtonian continuum) energy during shear flow, Avazmohammadi and Castaneda36 estimated the viscosity of soft particles dispersions in Newtonian continuum as function of the dimensionless parameter Ca defined in eqn (5), as shown below:
Ca = ηc/G | (5) |
Finally, a rough estimation of the shear rates in the elastohydrodynamic lubrication regime is possible by using the expression provided by de Vicente et al.35 for the central film thickness as shown in eqn (6).
hc = 3.3R′Ū0.6−0.14 | (6) |
Dispersion | Viscosity (Pa) | U (m s−1) | h c (m) | Shear rate (s−1) |
---|---|---|---|---|
50CS10 | 0.017 | 0.10 | 7.4 × 10−6 | 6764.0 |
75CS | 0.050 | 0.03 | 6.9 × 10−6 | 2187.0 |
75CS15 | 0.025 | 0.07 | 7.5 × 10−6 | 4653.0 |
1XG15 | 0.200 | 0.10 | 3.2 × 10−5 | 1541.0 |
Microgels enhanced the rheological and tribological performance when dispersed in continuum with relatively low viscosity, increasing the viscosity i.e., acting as thickeners, thus lowering the friction coefficient. However in high viscosity continuum, microgels caused a detrimental effect in mechanical performance, decreasing the viscosity and increasing the friction coefficients. Regardless of being dispersed in Newtonian or non-Newtonian continuum, increasing the elastic modulus of the microgels (higher protein content) showed benefits by increasing the high shear rate viscosity of the final dispersion. This consequently improved their tribological performance, i.e., decreased the friction coefficients. Thus, in order to obtain a benefit from microgels on the tribology performance of a dispersion, it is necessary to have harder particles when the continuum viscosity is increased. However, further studies are necessary to determine when microgels become too hard that they can increase the abrasiveness in biological contacts. Future work should look into a more thorough calculation of the effective volume fraction of the soft microgel particles in order to compare with theoretical models.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sm01802f |
This journal is © The Royal Society of Chemistry 2019 |