Jesús M.
Dones
ab,
I. Caglar
Tanrikulu
ac,
Jenu V.
Chacko
d,
Alexandra B.
Schroeder
e,
Trish T.
Hoang
c,
Angela L. F.
Gibson
*f,
Kevin W.
Eliceiri
*de and
Ronald T.
Raines
*abc
aDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. E-mail: rtraines@mit.edu
bDepartment of Chemistry, University of Wisconsin – Madison, 1101 University Avenue, Madison, WI 53706, USA
cDepartment of Biochemistry, University of Wisconsin – Madison, 433 Babcock Drive, Madison, WI 53706, USA
dDepartment of Biomedical Engineering, University of Wisconsin – Madison, 1550 Engineering Drive, Madison, WI 53706, USA
eDepartment of Medical Physics, University of Wisconsin School of Medicine and Public Health, 1111 Highland Avenue, Madison, WI 53705, USA. E-mail: eliceiri@wisc.edu
fDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, USA. E-mail: gibson@surgery.wisc.edu
First published on 30th October 2019
Collagen is an abundant component of the extracellular matrix and connective tissues. Some collagen-mimetic peptides (CMPs) that do not form homotrimers can anneal to damaged tissue. Here, through a computational screen, we identify (flpHypGly)7 as an optimal monomeric CMP for heterotrimer formation. We find that (flpHypGly)7 forms stable triple helices with (ProProGly)7 but not with itself. The nonnatural amino acid HflpOH, which is (2S,4S)-4-fluoroproline, is not toxic to human fibroblasts or keratinocytes. Conjugation of (flpHypGly)7 to a fluorescent dye enables the facile detection of burned collagenous tissue with high specificity. The ubiquity of collagen and the prevalence of injuries and diseases that disrupt endogenous collagen suggests widespread utility for this approach.
Natural collagen is comprised of three left-handed, polyproline II-type (PPII) helices aligned in a parallel manner and coiled with each other to form a right-handed triple helix.1,8 Each of the peptide strands is constituted by a continuous repeat of Xaa-Yaa-Gly units. Even though the Xaa and Yaa positions can accommodate any amino acid, (2S)-proline (Pro; 28% abundance) and (2S,4R)-4-hydroxyproline (Hyp; 38% abundance) are the most prevalent, respectively. As a result, ProHypGly is the most common triplet in collagen (10.5%).9 These natural preferences at the Xaa and Yaa positions promote the conformational stability of the collagen triple helix.1
Collagen stability is governed by stereoelectronic effects that preorganize its prevalent pyrrolidine rings.1 A Cγ-endo and Cγ-exo pyrrolidine-ring pucker is preferred at the Xaa and Yaa position, respectively, in stable triple-helical structures. Substituents at the 4-position of a proline residue can enforce the preferred pyrrolidine-ring pucker via a gauche10 or steric11 effect, lowering the entropic cost for forming a triple helix and increasing its conformational stability (Table 1).1 For example, the stereospecific installation of a fluoro group generates a gauche effect that leads to a Cγ-endo pucker in (2S,4S)-4-fluoroproline (flp) and supports the Cγ-exo pucker in (2S,4R)-4-fluoroproline (Flp) to a greater extent than does the 4R-hydroxy group of Hyp.12–14 The impact of such substitutions are apparent in the thermostability of collagen-mimetic peptides (CMPs). The value of Tm, which is the temperature at the midpoint of the thermal transition between a triple helix and its component strands, tracks the strength of the inductive effect induced by the substitutions.15 For example, replacing Hyp with Flp at the Yaa position in (ProYaaGly)7 leads to ΔTm = 9 °C (Table 1).
Xaa (R1,R2) | Yaa (R1,R2) | T mb (°C) | |||||||
---|---|---|---|---|---|---|---|---|---|
Flp (F,H) | Mop (OMe,H) | Hyp (OH,H) | Mep (H,CH3) | Clp (Cl,H) | Pro (H,H) | (XaaProGly)7 | (XaaProGly)10 | ||
a Values of IE were calculated with molecular mechanics. Values <2.8 kcal mol−1 are depicted in gray typeface (see note §). b Values of Tm for the denaturation of the indicated triple helices were determined previously by circular dichroism spectroscopy.1 ND, not determined; NH, no helix. | |||||||||
flp (H,F) | 2.8 | 3.4 | 3.0 | 1.1 | 5.6 | 1.3 | 33 | 58 | |
mep (CH3,H) | −0.4 | −0.3 | −0.1 | −0.6 | 2.4 | −0.4 | 13 | ND | |
Pro (H,H) | −0.1 | −0.6 | 0.3 | −0.2 | 1.2 | 0.0 | NH | 31–41 | |
T m b(°C) | |||||||||
(ProYaaGly)7 | 45 | ND | 36 | 29 | 23 | NH | |||
(ProYaaGly)10 | 91 | 70 | 61–69 | ND | 52 | 31–41 |
Not all Xaa and Yaa pairings are compatible. The register of the strands in a triple helix is staggered such that each cross-section contains an Xaa, Yaa, and Gly residue.1 Even though (flpProGly)7 and (ProFlpGly)7 form highly stable triple helices (Table 1),16 (flpFlpGly)7 is a monomer in solution due to unfavorable steric interactions between neighboring flp and Flp residues (Fig. 1).17 Still, mixtures of (flpFlpGly)7 and (ProProGly)7 produce stable triple helices because of the preorganization of (flpFlpGly)7 and the minimal steric clashes between flp/Flp and neighboring Pro residues.17
Fig. 1 Molecular models of neighboring Xaa and Yaa positions on strands of a collagen triple helix. (A) When Xaa and Yaa are Pro, there is not a steric clash between the neighboring side chains (cyan). (B) When flp and Flp are placed similarly, clashing fluoro groups (cyan spheres) deter strand-association.17 |
Because (flpFlpGly)7 and (ProProGly)7 form only heterotrimeric triple helices,‡ they can anneal readily to exposed strands on denatured collagen (Fig. 2). Such annealing could allow for the development of diagonistic or therapeutic agents conjugated to a monomeric CMP.19–22 Previously, we have used CMP-conjugates to deliver fluorescent dyes,23 a sunscreen,24 and growth factors25 to damaged collagen. Although (flpFlpGly)7 seemed to outperform (ProProGly)7 as an annealing strand,23 its exceptional propensity was hindered by its poor solubility, which can complicate formulation and dosing, and diminish bioavailability.26 The highly soluble (ProHypGly)7 peptide can be successful in this context, but its homotrimerization necessitates a denaturation step prior to application,27,28 complicating its use. Another approach invokes protecting groups to deter triple-helix formation,29,30 but their removal introduces operational complexity.
Fig. 2 Representation of a collagen-mimetic peptide with a pendant moiety (XCMP) annealing selectively to a damaged site within the collagen triple helix but not forming a homotrimeric triple helix. |
Here, we conduct a computational survey of proline analogs to identify (XaaYaaGly)7 variants that are monomeric in solution, but have high ability to anneal with damaged collagen. We confirm the association characteristics of our design in vitro and demonstrate its specificity for damaged collagen by identifying a burned region on a rat-tail tendon ex vivo.
Interaction energies (IE) revealed that four combinations—Xaa flp⋯Flp, flp⋯Mop, flp⋯Hyp, and flp⋯Clp—engender unfavorable interactions (Table 1).§ Among these four, a flp⋯Flp pair of Xaa⋯Yaa residues (IE = 2.8 kcal mol−1) merits special consideration. flp in the Xaa position and Flp in the Yaa position are individually the most stabilizing 4-substituted proline analogs. Yet, (flpFlpGly)7 does not form a stable triple helix, suggesting that Xaa⋯Yaa clashes of IE ≥ 2.8 kcal mol−1 are likely to produce monomeric peptides. Peptides that satisfied this criterion include (flpMopGly)7, (flpHypGly)7, and (flpClpGly)7 (IE = 3.4, 3.0, and 5.6 kcal mol−1, respectively). The higher solubility bestowed by Hyp (hydroxy group) compared to Mop (methoxy group) or Clp (chloro group) led to explore (flpHypGly)7 experimentally.¶ Notably, a stereospecific H→F substitution is the only difference between a flp-Hyp-Gly unit and the isologous Pro-Hyp-Gly unit of natural collagen. Moreover, deleterious O)(F steric clashes engendered by (flpHypGly)7 will not preclude annealing to strands of human collagen, which lack flp entirely and have Hyp in only 38% of its Xaa-Yaa-Gly units.9 Finally, the HflpOH monomer is readily accessible from natural HHypOH by an SN2 reaction.35 We note that CMPs containing flp-Hyp-Gly units have been examined previously,36,37 though in contexts distinct from those described herein. For example, (flpHypGly)9 was found to form a stable triple helix at ambient temperature, complicating its use in biological contexts.37 Hence, we examined a shorter peptide: (flpHypGly)7.
CD spectra for (flpHypGly)7 and (ProProGly)7 reveal shallow peaks near 225 nm (Fig. 3A). These samples exhibit no cooperative denaturation with increasing temperature, indicating the absence of triple helices in solution (Fig. 3B). This behavior is typical for PPII structures that do not associate into triple helices.36–38 In marked contrast, a 1:2 (flpHypGly)7/(ProProGly)7 solution exhibits a strong collagen signature in its CD spectrum (Fig. 3A), as well as cooperative denaturation with Tm = 28 °C (Fig. 3B and S2A†). A 2:1 (flpHypGly)7/(ProProGly)7 solution exhibits similar thermostability (Fig. S1A, S1B, and S2B†). The results of these mixing experiments indicate that (flpHypGly)7 can form a stable triple helix with (ProProGly)7 strands, but not with itself.
To mimic the attributes of the (flpHypGly)7 peptide, we synthesized (HypflpGly)7, which is a compositional isomer (CI). Swapping the flp and Hyp residues generates a sequence that violates the conformational restrictions of the Xaa and Yaa positions (Table 1), and should preclude triple-helix formation.12,39 Indeed, the CD spectrum of (HypflpGly)7 mimics closely that of (flpHypGly)7 (Fig. 3C), and does not exhibit the cooperative transition of triple-helix denaturation (Fig. 3D). Further, the CD spectrum of a 1:2 or 2:1 (HypflpGly)7/(ProProGly)7 mixture is a linear combination of the individual (ProProGly)7 and (HypflpGly)7 spectra and exhibits no evidence of a triple helix (Fig. 3C and S1C†). These spectra, together with the absence of a cooperative transition (Fig. 3D and S1D†), provide strong evidence that (HypflpGly)7 does not interact with (ProProGly)7. Because (HypflpGly)7 has the same composition as does (flpHypGly)7 but lacks its annealing properties, (HypflpGly)7 serves as an ideal negative control for our physiological experiments.
Fig. 4 Graph showing the effect of HflpOH on the viability of human fibroblasts (A) and primary human epidermal keratinocytes (B). Data were obtained with a tetrazolium dye-based assay for metabolic activity.44 Values are the mean ± SEM (n = 3, biological replicates). |
Fig. 5 Multiphoton microscopy images of the annealing of fluorescently labeled CMP and its compositional isomer to collagen fibers from rat tails. (A) Amino acid sequences of the Cy5CMP and Cy5CI peptides. (B) Method for probing for damaged collagen in a rat-tail tendon. (C) Superimposed images of a damaged rat-tail tendon using second harmonic generation (SHG) in the green channel and the fluorescence from Cy5CMP in the red channel. (D) Superimposed images of the SHG channel of a damaged rat-tail tendon and the fluorescence from the compositional isomer, Cy5CI. (E) Bar graph of the mean fluorescence intensity in the indicated regions of interest (ROIs) as determined with ImageJ software.48 |
Second harmonic generation (SHG) microscopy is a nonlinear optical method that is used extensively to identify and visualize fibrillar collagens in tissues.45–47 Using a multiphoton laser-scanning microscope, data can be acquired from both SHG and Cy5 channels, revealing the distinction between damaged and healthy collagen (SHG) and its detection with our probe (Cy5). The fibrils exposed to 350 °C show little or no signal through the SHG channel, confirming the absence of intact collagen structure in the burn area (Fig. S3†). Upon treatment with Cy5CMP, the regions that fail to show a strong SHG signal exhibit a strong Cy5 fluorescence (Fig. 5C), indicating the association Cy5CMP with damaged collagen. Importantly, the same intensity is not observed in intact regions. In contrast, the distinction between damaged and healthy collagen is not apparent with the negative control, Cy5CI (Fig. 5D).
Quantification of Cy5 fluorescence reveals that the localization of Cy5CMP on burned collagen is greater by 25-fold (i.e., 148/6) than its fluorescence on intact collagen (Fig. 5E). Similarly, the labeling of these regions by Cy5CMP is greater by 5.3-fold (i.e., 148/28) that is that by the Cy5CI peptide. These results confirm annealing by (flpHypGly)7 to regions of collagen damage. Finally, we note that Cy5CMP is highly soluble in water, showing no evidence of precipitation despite its pendant hydrophobic dye.
Footnotes |
† Electronic supplementary information (ESI) available: Experimental procedures and additional analytical data. See DOI: 10.1039/c9ob01839e |
‡ Neither (flpFlpGly)7 nor (ProProGly)7 forms a stable homotrimeric triple helix, though for different reasons. (flpFlpGly)7 strands are highly preorganized for triple-helix formation, but experience strong interstrand F)(F clashes in a triple helix.17 (ProProGly)7 strands do not engender steric clashes upon association, but are not especially well-organized for triple-helix formation.18 |
§ We also tested clp (R1 = H, R2 = Cl) as a candidate at the Xaa position. Its use, however, produces extremely unfavorable interactions, even when its neighbor is Pro (IE = 13.8 kcal mol−1). This incompatibility is consistent with the low thermostability of triple helices with clpProGly units,34 and disqualifies the use of clp at the Xaa position of an annealing strand. |
¶ LogP values are HHypOH, −2.64; HMopOH, −2.02; HProOH, −1.72; HFlpOH, −1.71; and HClpOH, −1.40; as calculated with software from Molinspiration (Slovenský Grob, Slovak Republic). |
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