Feng
Yu
ab,
Xiaodong
Cao
*abc,
Yuli
Li
abc,
Lei
Zeng
ac,
Bo
Yuan
ab and
Xiaofeng
Chen
*abc
aSchool of Materials Science and Engendering, South China University of Technology, Guangzhou, 510641, PR China. E-mail: caoxd@scut.edu.cn; Fax: +86-20-87111752; Tel: +86-20-22236066
bNational Engineering Research Centre for Tissue Restoration and Reconstruction, Guangzhou, 510006, PR China. E-mail: chenxf@scut.edu.cn; Fax: +86-20-22236083; Tel: +86-20-22236283
cGuangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510640, PR China
First published on 6th July 2018
Correction for ‘An injectable hyaluronic acid/PEG hydrogel for cartilage tissue engineering formed by integrating enzymatic crosslinking and Diels–Alder “click chemistry”’ by Feng Yu et al., Polym. Chem., 2014, 5, 1082–1090.
In order to further prove the authenticity of the experimental data, additional supplementary information is also provided here.
Fig. S1(A–C) are the original images generated using fluorescence microscopy. Because of the high cell culture density encapsulated in the hydrogel (3 × 106 cells per ml), the phenomenon of cell proliferation after 1 d, 3 d and 7 d culture cannot be distinguished and observed especially in low brightness. Therefore, the image brightness was increased to 20% and 40% to make sure that more cells (marked with green fluorescence) could appear in the image and so that the cell proliferation after different culture days could be observed by the naked eye (Fig. S1(D–I)).
In addition, a CCK-8 assay is an efficient and quantitative way to prove the cell proliferation. Here, the detailed data are also provided in Table S1 to further supplement the data shown in Fig. 8D.
OD value | 1 day | 3 days | 5 days | 7 days |
---|---|---|---|---|
Parallel group 1 | 0.68284 | 0.981505 | 1.39048 | 1.848 |
Parallel group 2 | 0.723215 | 1.02393 | 1.431505 | 1.94505 |
Parallel group 3 | 0.731945 | 0.921341 | 1.429301 | 1.79301 |
Parallel group 4 | 0.703852 | 0.843448 | 1.321341 | 1.96406 |
Average value | 0.710463 | 0.942556 | 1.393157 | 1.88753 |
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