Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

Yanli Ma; Zhehao Lv; Tingyu Li; Tian Tian; Lianyu Lu; Wenli Liu; Zhi Zhu; Chaoyong Yang

doi:10.1039/C8CC05037F

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions†

Yanli Ma,^a Zhehao Lv,^a Tingyu Li,^a Tian Tian,^a Lianyu Lu,^a Wenli Liu,^a Zhi Zhu^a and Chaoyong Yang

*^ab

Author affiliations

* Corresponding authors

^a State Key Laboratory of Physical Chemistry of Solid Surfaces, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
E-mail: cyyang@xmu.edu.cn

^b Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China

Abstract

ortho-Phthalaldehyde (OPA) phosphoramidite with high reaction activity was designed and synthesized for labelling oligodeoxynucleotides (DNA). The DNA modified with OPA (OPA-DNA) can covalently couple with native proteins rapidly and efficiently via a condensation reaction with the formation of phthalimidines, which provides a highly efficient method for bioconjugation of DNA and native proteins under physiological conditions.

Download options Please wait...

Supplementary files

Supplementary information PDF (765K)

Article information

DOI: https://doi.org/10.1039/C8CC05037F
Article type: Communication
Submitted: 26 Jun 2018
Accepted: 30 Jul 2018
First published: 30 Jul 2018

Download Citation

Chem. Commun., 2018,54, 9434-9437

Permissions

Request permissions

Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

Y. Ma, Z. Lv, T. Li, T. Tian, L. Lu, W. Liu, Z. Zhu and C. Yang, Chem. Commun., 2018, 54, 9434 DOI: 10.1039/C8CC05037F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions

Social activity

Search articles by author

Spotlight

Advertisements