Issue 58, 2018
From the journal:

Chemical Communications

Transpeptidation-directed intramolecular bipartite tetracysteine display for sortase activity assay†

Ai-jun Xu,ab   Yong Yang*b  and  Chun-Yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 0531-82615258
Tel: +86 0531-86186033

b Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
E-mail: yong.yang1@siat.ac.cn
Fax: +86 0755-86392229
Tel: +86 0755-86585240

Abstract

We developed a label-free method to measure sortase activity by using transpeptidation-directed intramolecular bipartite tetracysteine display. This assay is homogenous with a detection limit of 10.7 ng mL−1, and it can be applied to characterize Sortase A (SrtA)-targeted inhibitors, providing a new approach for anti-virulence drug discovery.

Graphical abstract: Transpeptidation-directed intramolecular bipartite tetracysteine display for sortase activity assay

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Article information

DOI
https://doi.org/10.1039/C8CC04495C
Article type
Communication
Submitted
06 Jun 2018
Accepted
26 Jun 2018
First published
28 Jun 2018

Chem. Commun., 2018,54, 8116-8119

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Transpeptidation-directed intramolecular bipartite tetracysteine display for sortase activity assay

A. Xu, Y. Yang and C. Zhang, Chem. Commun., 2018, 54, 8116 DOI: 10.1039/C8CC04495C

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