A highly sensitive and selective fluorescent probe for fast sensing of endogenous HClO in living cells

A new fluorescence probe was developed for HClO detection under physiological conditions, which displayed fast response (t_1/2 < 30 s) and large off–on fluorescence increase (>1000-fold) toward HClO with high sensitivity and selectivity. The probe was successfully applied for the detection of both exogenous and endogenous HClO in living cells. Using the probe-based tool, we could observe the H₂S-induced HClO biogenesis in living cells for the first time.