Issue 30, 2018

A novel near-infrared probe for the imaging of superoxide anion fluctuations and hydrogen ion enhancement in vivo

Abstract

Superoxide anions (O2˙) and hydrogen ions (H+) have attracted considerable attention in studies on cellular dysfunction. A newly synthesized reaction-based near-infrared imaging probe (IR-747-SAPH), which is composed of a superoxide anion responsive group and a H+ responsive group, exhibits high selectivity to O2˙ and H+ fluxes in vivo. The fluorescence intensity of IR-747-SAPH changes as O2˙ and H+ concentrations vary. IR-747-SAPH is characterized by sensitivity to changes in H+, high photostability, and strong near-infrared properties, including high temporal resolution, deep penetration, and low background interference. The probe also provides reliable near-infrared fluorescence signals that alter O2˙and H+ levels in vivo. Therefore, IR-747-SAPH fluorescence imaging is a promising detection method for monitoring the changes in O2˙ and H+ in various biological environments.

Graphical abstract: A novel near-infrared probe for the imaging of superoxide anion fluctuations and hydrogen ion enhancement in vivo

Supplementary files

Article information

Article type
Paper
Submitted
25 May 2018
Accepted
29 Jun 2018
First published
03 Jul 2018

Anal. Methods, 2018,10, 3727-3736

A novel near-infrared probe for the imaging of superoxide anion fluctuations and hydrogen ion enhancement in vivo

C. Sun, W. Du, W. Zhang, Y. Wu, Z. Yao, B. Wang, T. Wu, H. Yang, Y. Wang and L. Ren, Anal. Methods, 2018, 10, 3727 DOI: 10.1039/C8AY01190G

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