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We have rationally constructed a new two-photon fluorescent hypochlorous acid probe (TP-HA). The probe TP-HA displayed a fluorescence enhancement (∼16-fold) in response to HOCl with high sensitivity and selectivity. The new probe was membrane-permeable and suitable for visualization of the changes of HOCl in living cells and tissues.

Graphical abstract: Preparation of a two-photon fluorescent probe with a large turn-on signal for imaging hypochlorous acid in living tissues

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