A simple fluorescent aptamer based assay coupled with fluorescence scanning capillary array for aflatoxin B1

Abstract

We developed a simple aptamer fluorescence assay for aflatoxin B1 (AFB1) detection by using an array of capillaries. The 34-nt aptamer having a single fluorescein (FAM) label on the 24th T nucleotide generated a remarkable fluorescence increase upon AFB1 binding. The use of fluorescence scanning capillary array allowed for the analysis of multiple samples with low sample consumption, showing advantages of simplicity, rapidity and high throughput analysis. The detection limit of AFB1 reached 0.5 nM. This assay has great potential for analysis in food safety and environmental monitoring.