Issue 19, 2017

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

Abstract

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.

Graphical abstract: Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

Supplementary files

Article information

Article type
Communication
Submitted
14 Dec 2016
Accepted
13 Feb 2017
First published
13 Feb 2017

Chem. Commun., 2017,53, 2874-2877

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

H. Soleimaninejad, M. Z. Chen, X. Lou, T. A. Smith and Y. Hong, Chem. Commun., 2017, 53, 2874 DOI: 10.1039/C6CC09916E

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