Ratiometric fluorescence monitoring of cerebral Cu2+ based on coumarin-labeled DNA coupled with the Cu2+-induced oxidation of o-phenylenediamine

Shishi Ma; Qiao-Yu Zhou; Fang-Ya Mu; Zi-Han Chen; Xu-Yin Ding; Min Zhang; Guoyue Shi

doi:10.1039/C7AN01099K

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Ratiometric fluorescence monitoring of cerebral Cu²⁺ based on coumarin-labeled DNA coupled with the Cu²⁺-induced oxidation of o-phenylenediamine†

Shishi Ma,^a Qiao-Yu Zhou,^a Fang-Ya Mu,^a Zi-Han Chen,

^a Xu-Yin Ding,^a Min Zhang

*^a and Guoyue Shi*^a

Author affiliations

* Corresponding authors

^a School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China
E-mail: mzhang@chem.ecnu.edu.cn, gyshi@chem.ecnu.edu.cn

Abstract

A novel and facile ratiometric fluorescence method for evaluating Cu²⁺ has been developed based on coumarin-labeled single-stranded DNA (C-ssDNA) coupled with the Cu²⁺-induced oxidation of o-phenylenediamine (OPD). By combining the microdialysis technique, the ratiometric fluorescence method has also been successfully exploited to monitor the cerebral Cu²⁺ in the rat brain, promising new opportunities for studying the cerebral Cu²⁺-associated physiological and pathological events.

This article is part of the themed collection: Recent analytical chemistry science from China

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Article information

DOI: https://doi.org/10.1039/C7AN01099K
Article type: Communication
Submitted: 01 Jul 2017
Accepted: 02 Aug 2017
First published: 04 Aug 2017

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Analyst, 2017,142, 3341-3345

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Ratiometric fluorescence monitoring of cerebral Cu²⁺ based on coumarin-labeled DNA coupled with the Cu²⁺-induced oxidation of o-phenylenediamine

S. Ma, Q. Zhou, F. Mu, Z. Chen, X. Ding, M. Zhang and G. Shi, Analyst, 2017, 142, 3341 DOI: 10.1039/C7AN01099K

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