Hannes Orelma‡
*ab,
Maija Vuoriluotoa,
Leena-Sisko Johanssona,
Joseph M. Campbella,
Ilari Filpponena,
Markus Biesalskib and
Orlando J. Rojasa
aBiobased Colloids and Materials (BiCMat), Aalto University, School of Chemical Technology, Department of Forest Products Technology, 00076 Aalto, PL 11000, Espoo, Finland. E-mail: hannes.orelma@vtt.fi
bLaboratory of Macromolecular Chemistry and Paper Chemistry, Department of Chemistry, Technische Universitat Darmstadt, 64287 Darmstadt, Germany
First published on 2nd September 2016
A method for preparing photo-crosslinkable cellulose nanofibrils (CNF) was investigated. Benzophenone (BP), a UV-radical crosslinker, was chemically grafted to TEMPO-oxidized wood fibers in water/DMSO medium. This resulted in a reduction of carboxyl group content together with an increase of the number density of amide linkages. The BP-functionalized fibres were then microfluidized into TEMPO-oxidized CNF (TOCNF). As evidence of the crosslinking performance, the films of BP-activated TOCNF displayed improved water resistance upon UV curing (even under high energy sonication). In addition to improving wet strength the method is suitable for further modification of CNF, either by utilizing the remaining free carboxyl groups or via photochemical grafting of other substances onto the CNF structure.
When cellulosic materials are fibrillated into CNF, carboxyl groups play an important role in easing the disintegration process of fibrils by increasing swelling and inter fibrillar electrostatic repulsions, thus lowering demands in mechanical energy.8 Moreover, carboxyl groups are useful in gel formation and in grafting or conjugating functionalities onto CNF. However, high carboxyl content in CNF may compromise the wet strength of the material.
Wet strength of CNF may be improved by addition of cationic or non-anionic polymers.9,10 However, other routes may be desirable when bi-functional CNF-materials are required, for example if carboxyl (or other charged) groups are needed for activation in addition to e.g. water resistance. Light-induced crosslinking is a relatively new approach for improving the wet strength of cellulosic materials, especially if achieved without addition of chemical additives. For example benzophenone (BP) can provide radical-based crosslinking under UV irradiation.11 Benzophenone grafted polymers have been found to work effective with polymeric thin films in grafting polymeric materials and internally cross-linked polymeric layers12,13 and BP is widely utilized for UV protection of plastics, soaps, and perfumes.
Under UV-exposure, benzophenone photolyse to a highly reactive triplet state, a ketone intermediate via n–π* or π–π* transition, depending on the UV-light wave length.14 Benzophenone radicals can react with hydrogens from aliphatic CH groups via H-abstraction/recombination mechanisms. Moreover, unreacted BP molecules can undergo multiple photolytic activation cycles improving the conjugation efficiency. The conjugation of BP onto cellulose has been recently introduced for UV-crosslinking cellulose nanocrystal (CNC) films, by covalently grafting benzophenone on surfaces of CNC.15 Moreover, benzophenone has been used with functional paper devices as an UV-reactive crosslinker by incorporating benzophenone into methyl methacrylate polymer chain.16,17 When a benzophenone molecule is grafted onto cellulose, under UV-exposure the radicalized benzophenone substitute may abstract hydrogen from both C1-carbon and C6-ketone (CO) of a cellulose glucopyranose ring located to the neighbouring cellulose chain leading to a covalent bond formation. When benzophenone is located onto CNF fibril, fibrils carrying benzophenone substituents may covalently attach to neighbouring fibrils unsubstituted spots.
In this work, BP was covalently grafted onto CNF using a tailored sequential pre-functionalization strategy. First the precursor wood fibers were TEMPO-oxidized to open their structure. Then BP was grafted onto the carboxylic groups via aqueous EDC/NHS coupling (Scheme 1) and only after functionalisation the fibers were disintegrated into cellulosic nanofibrils (TEMPO-oxidized CNF or TOCNF) using high pressure fluidization. Prepared TOCNF (BP-TOCNF) was cast into films and crosslinked using UV light. The method presented herein is not only suitable for improving the wet strength of CNF but can also potentially be utilized for the photochemical grafting of various monomeric or polymeric additives onto the CNF backbone. This developed strategy is proposed for greener nanolignocellulosic materials.
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Scheme 1 Schematic illustration on the manufacture of benzophenone functionalized nanocellulose (BP-TOCNF) materials. |
The surface composition of self-supporting CNF films was analysed by a Kratos Analytical AXIS Ultra X-ray photoelectron spectrometer with a monochromatic Al Kα X-ray source at 100 W and a neutralizer. The XPS experiments were performed with dry TOCNF films (pre-evacuated overnight). At least three spots of each sample were analyzed. Spectra were collected at an electron take-off angle of 90° from sample areas less than 1 mm in diameter. Elemental surface compositions were determined from low-resolution measurements (160 eV pass energy and 1 eV step) while the surface chemistry was probed with high resolution measurements (20 eV pass energy and 0.1 eV step). The carbon C 1s high-resolution spectra were curve fitted using parameters defined for cellulosic materials.19 All binding energies were referenced to the aliphatic carbon component of the C 1s signal at 285.0 eV. The UHV conditions remained satisfactory during the XPS experiments as per a reference used in situ (100% cellulose ash-free filter paper) that was measured along with each sample batch.
The effect of BF content on material properties was evaluated using solvent cast films prepared from mixtures of neat and BP treated NCF. Mixed TOCNF/BP-TOCNF films with and without UV-activation were placed in water for 2 days and then the films were analysed visually while wet.
Benzophenone was grafted onto the carboxylated fibers via EDC/NHS activation from DMSO/water mixture. Since benzophenone is not water soluble, the reaction was carried out in water with the addition of a small amount of DMSO in which the BP was dissolved prior to the reaction. Note, DMSO has also been reported to swell wood fibers, possibly increasing the accessibility of the carboxylic groups on the cellulosic fibers to which the BP was linked through an amidation reaction.1 During the amidation reaction the fibers turned slightly yellow (Fig. 1a and b). After reaction, the BP-modified wood-fibers (Fig. 1c) were disintegrated with a fluidizer, and then the disintegrated BP-TOCNF was casted into the films by using pressurized filtration as described.3
The AFM height image of BP-TOCNF shows a typical fibrillar structure (Fig. 1d), which is rather similar to that of unmodified TOCNF (Fig. S1†).21 The BP-TOCNF were approximately 3–4 nm thick, as determined by the Nanoscope analysis software. Interestingly BP-TOCNF fibrils were slightly straighter than unmodified TOCNF fibrils. This could be explained if BP grafting altering TOCNF hydrophobicity which would make them more rigid when being in contact with water.
Carboxyl contents of TEMPO-oxidized fibers before and after conjugation of BP are shown in Fig. 2a. Due to the EDC/NHS coupling of the aminobenzophenone to the surface carboxyl groups the amount of charges decreased by ca. 70% during the reaction, indicating large degree of conversion of BP-linkage. However, significant amount of free carboxyl groups remained on the fiber surface. These would be beneficial in the following disintegration step, as they would improve colloidal stability and swelling.8 The amide bonds formed during the conjugation reaction were further investigated with ATR-FTIR, see Fig. 2b. Typical peaks for amide bonds were observed: 1645 cm−1 (amide I band, CO stretching) and 1549 cm−1 (amide II band, combined N–H deformation and C–N stretching), further confirming the linking of benzophenone. Moreover, the characteristic C
O peak (1603 cm−1) of sodium carboxylate22 decreased remarkably when BP was grafted. We did not consider aldehydes produced during the alkaline TEMPO-oxidation that can raise the conjugation level of BP due to the nucleophile addition reaction between aldehydes and amines.23
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Fig. 2 Carboxyl content of TEMPO-oxidized fibers before and after conjugation of BP by conductometric titration (a). FTIR absorbance spectra of TOCNF and BP-TOCNF (b). |
XPS was utilized to investigate the surface compositions of the TONCF films with and without BP, see Fig. 3. TEMPO oxidation showed up as marked increase in elemental sodium and in aliphatic/aromatic carbon C 1s component at 285 eV even though carboxylic groups cannot be reliably detected in cellulose derivatives by XPS.24,25 In the film with BP nitrogen (N 1s, 2 at%) was also detected, while BP treated samples otherwise exhibited characteristics typical to cellulose.
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Fig. 3 XPS spectra of TOCNF with and without benzophenone substituents. Included are also the respective spectra of a cellulose standard. |
Water contact angle (WCA) measurements were carried out on TOCNF films with and without grafted BP (Fig. 4). As expected, the contact angle of BP-TOCNF was remarkably higher than that of TOCNF (WCA after 30 s for TOCNF and BP-TOCNF were approximately 15 and 50 degrees, respectively). In the literature, CNF films before and after TEMPO-oxidation have been reported to be 10–35 and 40 degrees, respectively.3,26,27 The observed static CA for BP-TOCNF is, nevertheless, lower than that of hydrophobic octadecylamine substituents that raised the CA of TOCNF to 108 degrees.26 So, the FTIR, XPS, and WCA observations together yield evidence that BP was grafted onto the carboxylated fibrils.
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Fig. 4 Water contact angles as a function of time of TOCNF (bottom curve) and BP-TOCNF (upper profile). |
The physical properties of pressure filtered TOCNF films with and without BP substituents are summarized in Table 1. The observed density for the non-modified TOCNF (1.005 g cm−3) was lower than the reported value for films made from mechanical disintegrated CNF, 1.080–1.200 g cm−3 and TEMPO-oxidized CNF, 1.450 g cm−3.28,29 This is probably due to the film manufacturing method: in this study films were prepared with the pressure filtration and while in the above mentioned studies solvent casting was typically utilised, and the latter leads to higher CNF film densities. However, the density of BP-TOCNF films was still much lower (only 0.664 g cm−3), and in addition to this, the BP grafted TOCNF films were much thicker (more than 50%) than the unmodified TOCNF films. Both observations suggest that hydrophobicity induced by BP grafting made the nanofibrillated fibres more rigid in water.
Density (g cm−3) | Thickness (μm) | Specific tensile strength (MPa cm3 g−1) | Elongation (%) | |
---|---|---|---|---|
TEMPO-CNF | 1.0 ± 0.38 | 54 ± 2 | 109 ± 10 | 3.3 ± 0.6 |
BP-CNF without UV | 0.66 ± 0.32 | 84 ± 4 | 114 ± 23 | 2.7 ± 1.2 |
BP-CNF with UV | 0.70 ± 0.44 | 80 ± 5 | 138 ± 10 | 2.0 ± 0.5 |
The density variations were considered in the tensile strength measurements by normalizing the tensile strength by density (specific tensile strength). The strength values for the TOCNF observed are in the same range as reported in literature, 80–230 MPa for unmodified CNF,3,30 although direct comparison is difficult, due to variation in the experimental setup. Without UV-activation, no significant strengthening or changes in strain of the BP-TOCNF film were observed. However, after the UV-activation (UV-curing) the tensile strength of the BP-TOCNF films increased from 114 to 138 MPa cm3 g−1 while the elongation decreased slightly (from 2.7 to 2.0%). This observation strongly suggests the UV light catalysed radical crosslinking between cellulose nanofibrils and BP substituents.15
Preliminary tests on the effect of the UV-induced crosslinking on the BP-TOCNF film swelling in water was carried out, using films with different amounts of BP. The TOCNF/BP-TOCNF films with and without UV-activation were placed in water for two days and then the films were analysed visually while still wet (Fig. S2, ESI†). The thicknesses of the films without UV activation decreased slightly as a function of BP-TOCNF added (the thickness values were not measured since the films with low BP-content were too soft and brittle for accurate thickness measurements). This was due to higher hydrophobicity induced by BP. The effect of UV-induced crosslinking in the swelling of mixed films was remarkable: all films with added BP-TOCNF stayed thin compared to those without UV-activation. Furthermore, the thickness of a film made from 100% BP-TOCNF did not change during swelling in water, further supporting the hypothesis that UV-light induced covalent bond formation in BP-TOCNF films. In this study were did not consider the effect of wet pressing while the BP-TOCNF films were UV-activated. This could lead to a better crosslinking efficiency since fibrils are expected to be in closer proximity at the same time that radical-induced coupling reactions occur.
The effect of BP on CNF film stability in water was also investigated, using sonification tests. The sonication was carried out in the absence of mechanical contact with the tip, see images in Fig. S3.† As expected, films of TOCNF and BP-TOCNF without and with UV-activation disintegrated fully after immersion in water for 24 h and followed by 2 min sonication. Only the UV-activated BP-TOCNF film remained intact. Both, films of TOCNF and BP-TOCNF without UV-activation became dispersed within the first seconds of sonication. The observation that UV-induces BP-TOCNF crosslinking in the film correlated with the mechanical energy supplied to the film, which is roughly equal with results from BP-functionalized CNC.15 In this study BP was linked onto CNC through hydroxyl groups with 4-benzoylphenyl (6-isocyanatohexyl) carbamate (Bp-NCO), and no charged groups were introduced onto the CNC prior to the grafting of the BP groups. Further, in this latter study, the density of grafted hydroxyl groups was 157 μmol g−1, which is six fold lower compared to 957 μmol g−1, which we achieved in the presence of installed carboxyl groups. On the other hand, the large density of BP grafted on CNF reduces the hydrophilicity of the material (note the recorded contact angles). By adjusting the grafting conditions, it is possible to tune the interactions with water as required in different applications.
Footnotes |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c6ra15015b |
‡ Present address: VTT, Technical Research Centre of Finland, Biologinkuja 7, P.O. Box 1000, FIN-02044 VTT, Finland. |
This journal is © The Royal Society of Chemistry 2016 |