Study on an improved bio-electrode made with glucose oxidase immobilized mesoporous carbon in biofuel cells

Response surface methodology (RSM) was used for process optimization to immobilize glucose oxidase (GOx) on ordered mesoporous carbon (OMC). Results showed that the maximum GOx adsorption on OMC was 37.38 mg g⁻¹ OMC with the activity of 8531.59 U g⁻¹ GOx-immobilized OMC under the optimized conditions. The interaction effect between GOx, OMC concentration and NaCl and pH were observed. By immobilizing GOx on OMC, we can improve the retained catalyst activity at high temperature (37.3 °C) and shift the optimum reaction pH towards a high value (pH 7.2). The electrochemical characteristics of immobilized GOx can also be enhanced with higher current peak (85.12 μA cm⁻²) and lower reduction potential.