Characterization of the [3Fe–4S]0/1+ cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analyses

Lars Lauterbach; Leland B. Gee; Vladimir Pelmenschikov; Francis E. Jenney; Saeed Kamali; Yoshitaka Yoda; Michael W. W. Adams; Stephen P. Cramer

doi:10.1039/C5DT04760A

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Characterization of the [3Fe–4S]^0/1+ cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analyses†

Lars Lauterbach,*^ab Leland B. Gee,^a Vladimir Pelmenschikov,*^b Francis E. Jenney, Jr,^c Saeed Kamali,^ad Yoshitaka Yoda,^e Michael W. W. Adams^f and Stephen P. Cramer^ag

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of California, Davis, CA 95616, USA

^b Institut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany
E-mail: lars.lauterbach@tu-berlin.de, pelmentschikov@tu-berlin.de

^c Georgia Campus, Philadelphia College of Osteopathic Medicine, Suwanee, GA 30024, USA

^d Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee Space Institute, Tullahoma, TN 37388, USA

^e JASRI, SPring-8, Sayo-gun, Hyogo 679-5198, Japan

^f Department of Biochemistry & Molecular Biology, Life Sciences Building, University of Georgia, Athens, GA 30602, USA

^g Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA

Abstract

The D14C variant of Pyrococcus furiosus ferredoxin provides an extraordinary framework to investigate a [3Fe–4S] cluster at two oxidation levels and compare the results to its physiologic [4Fe–4S] counterpart in the very same protein. Our spectroscopic and computational study reveals vibrational property changes related to the electronic and structural aspects of both Fe–S clusters.

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Article information

DOI: https://doi.org/10.1039/C5DT04760A
Article type: Communication
Submitted: 06 Dec 2015
Accepted: 20 Mar 2016
First published: 23 Mar 2016

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Dalton Trans., 2016,45, 7215-7219

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Characterization of the [3Fe–4S]^0/1+ cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analyses

L. Lauterbach, L. B. Gee, V. Pelmenschikov, F. E. Jenney, S. Kamali, Y. Yoda, M. W. W. Adams and S. P. Cramer, Dalton Trans., 2016, 45, 7215 DOI: 10.1039/C5DT04760A

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