Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure†
Abstract
The activity of lysozyme over a Micrococcus lysodeikticus cell suspension increased to 13% of the initial value in the presence of 1% v/v ammonium-based ionic liquids after deactivation with 4.0 M urea. This increase in activity reflects the refolding ability of the ionic liquids against the denaturation effects of urea on lysozyme.