Issue 24, 2016

Label/quencher-free detection of single-nucleotide changes in DNA using isothermal amplification and G-quadruplexes

Abstract

We report an analytical method that exploits the interaction between G-quadruplexes and thioflavin T (ThT), for detecting mutant DNA species containing single-base changes. This system is a label/quencher-free fluorescence enhancement system based on rolling circle amplification (RCA)-responsive G-quadruplex formation, which enables a highly selective detection of T790M SNPs in the gene encoding the epidermal growth factor receptor, EGFR.

Graphical abstract: Label/quencher-free detection of single-nucleotide changes in DNA using isothermal amplification and G-quadruplexes

Supplementary files

Article information

Article type
Communication
Submitted
15 Jul 2016
Accepted
27 Oct 2016
First published
28 Oct 2016

Analyst, 2016,141, 6503-6506

Label/quencher-free detection of single-nucleotide changes in DNA using isothermal amplification and G-quadruplexes

I. J. Lee, N. Goo and D. Kim, Analyst, 2016, 141, 6503 DOI: 10.1039/C6AN01600F

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