Issue 18, 2016

Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

Abstract

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) is a technique well suited for analysis of biological specimens. This tutorial review focuses on recent advancements and applications of IR-MALDESI MSI to better understand key biological questions. Through optimization of user-defined source parameters, comprehensive and quantitative MSI data can be obtained for a variety of analytes. The effect of an ice matrix layer is well defined in the context of desorption dynamics and resulting ion abundance. Optimized parameters and careful control of conditions affords quantitative MSI data which provides valuable information for targeted, label-free drug distribution studies and untargeted metabolomic datasets. Challenges and limitations of MSI using IR-MALDESI are addressed in the context of the bioimaging field.

Graphical abstract: Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

Article information

Article type
Tutorial Review
Submitted
23 May 2016
Accepted
18 Jul 2016
First published
19 Jul 2016

Analyst, 2016,141, 5236-5245

Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

M. T. Bokhart and D. C. Muddiman, Analyst, 2016, 141, 5236 DOI: 10.1039/C6AN01189F

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